查询词典 mutagenesis

Methods Seventy-four strains of Salmonella enterica serovar enteritidis (S. enteritidis), S. pullorum and S, gallinarum isolated from chickens were determined for biofilm using crystal violet staining, and the strain C050041 with well biofilm formation was chosen to construct a mutant library using transposon mutagenesis.

利用结晶紫染色定量法对74株鸡源的肠炎、鸡白痢和鸡伤寒沙门氏菌进行生物膜测定，选择生物膜生长较好的肠炎沙门氏菌C050041，采用转座子随机插入法构建突变株库。

Results: The results showed that both the wild type of recombinant MLCK and the ATP binding site mutagenesis of MLCK expressed in E.coli quantifiably with a large amount and exist as a soluble form.

结果：实验结果显示重组MLCK和MLCK/△ATP在大肠杆菌中以可溶的形式大量表达；经CaM-Sepharose 4B和Superose 6 HR纯化，SDS-PAGE鉴定得到单一的表达条带。

Because of high reproductivity andtransparent embryos, zebrafish becomes the best vertebrate for mutagenesis.

由于具有产卵量大，胚胎透明易于观察等优点，斑马鱼成为脊椎动物中最好的诱变材料。

Finally, the saccharomycetes which had muted in the outward shape and ability of the ethyling alcohol had enhanced the heavy ion mutagenesis was a method for selective breeding fine mold mushroom spawn in the experiment.

实验证明，经过诱变后的菌种在外观形态和耐酒精性能以及产酒精能力上有提高，重离子诱变作为选育菌种的方法具有很好的效果。

Methods Concanavalin A, wheat germ agglutinin, Sambucus nigrn agglutinin, Ricinus communis agglutinin-I and phytohemagglutinin were labeled with horseradish peroxidase by an improved sodium periodate method, and the synthetic HRP-agglutinins were used for determination of the expression levels of polysaccharide in common α-hemolyic streptococcus and in the α-hemolyic streptococcus after space mutagenesis.

采用改良的过碘酸钠法将辣根过氧化物酶分别标记在刀豆凝集素、麦胚凝集素、接骨木凝集素、蓖麻凝集素I及植物血凝素上，合成HRP-凝集素，检测普通型α-溶血性链球菌及太空型链球菌菌体内多糖分子的表达水平。

The results were as followed: 1 Mutagenesis Breeding of High Tannase Activity Strains and preparing of Tannase As a original strain, Asp. niger CY was mutated by UV treatment. One strain was obtained from the mutants.

全文实验结果如下： 1 单宁酶菌种的选育和单宁酶的制备以一株产单宁酶的黑曲霉为出发菌株，进行了菌株的诱变选育，得到了一株单宁酶高产菌株。

Site-directed mutagenesis was used to change the gene encoding tryptic bovine liver microsomal hepatic Cyt 〓.

本论文采用寡聚核苷酸定点突变技术对人工合成的编码牛肝细胞色素〓的基因进行改变，得到了目标突变基因。

In order to investigate whether the lysine 188 residue in the XRCC4 is chemically modified by the enzyme SIRT2 or not, I apply site-directed mutagenesis to alter this lysine to either arginine or glutamine, which mimicking the non-acetylating or fully acetylating of lysine residue.

为探讨具酵素功能之SIRT2是否对於XRCC4 188位置之离胺酸进行化学修饰作用，利用定位突变方式分别将该离胺酸置换成麸醯胺或精胺酸，试图模仿XRCC4离胺酸188乙醯化与去乙醯化，是否造成其对SIRT2之亲和力之改变。

Screening a Aspergilli with lipid production of 13.52% from soil, a mutant of superior Aspergilli was obtained with high lipid production of 18.75% through ultraviolet mutagenesis in order to empolder microbial oils resource.

为开发微生物油脂资源，从土壤中筛选出1株油脂含量为13.52%的曲霉，经紫外线诱变选育出1株油脂含量为18.75%的高产油脂曲霉。

Now, Chemicals are the capital therapeutics to cure malignancy, but the exploitation cost of chemicals is expensive, it has big adverse reaction and most of it have mutagenesis noxious property.

目前恶性肿瘤的药物治疗中是以化学药品为主，但化学药品的开发费用昂贵，毒副作用大，多有不同程度的致突变毒性。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。