查询词典 mutagenesis

The results show that mutation with the method of the ultraviolet and acridine orange can get a better result during the forementioned several ways of mutagenesis.

结果表明在几个不同的诱变方法中，经紫外线和吖啶橙联合诱变后爱德华氏菌的弱毒效果较好。

For sodium azide and dexon,the minimum measurable mutagenesis dose were 0050 and 0080μg/bottle,respectively.

用本方法测得NaN3和敌克松的最低诱变剂量分别为0.050μg／瓶和0.080μg／瓶，灵敏度显著高于Ames试验。

Error catastrophe theory has led to a strategy of mutagenesis to control disease viruses.

错误突变理论引导了通过突变来控制致病病毒的策略。

In this study, the 8 cysteine residues in AtKu70 were mutated by means of serine-substituted mutagenesis.

另有研究指出，Ku70可以在没有Ku80的存在下，单独与DNA结合，但其结合能力比较微弱。

By using of transgenesis, cell mutagenesis, analysis of signal pathways and genes interaction, we developed novel methods of system biotechnology for drug discovery, transgenic bioreactor and isolation of differential expression genes involved in cytogenesis .

采用转基因细胞系、突变细胞系研究细胞发生的信号传导路径、多基因相互作用与生物系统的结构–图式形成，发展了系统生物学新技术进行基因克隆、药物筛选和生物反应器的方法。

Bamboo mosaic virus encodes a 155-kDa replicase that the middle region contains a helicase-like domain according to the existence of several conserved featured motifs of helicase. Previous studies showed that the helicase-like domain exhibits both ATPase and RNA 5'-triphosphotase activities, and Mg2+ is essential for both activities. Mutagenesis of conserved motifs showed these amino acids play important roles for enzymatic activities. These results suggested that the properties of this denatured and renatured protein are coincident with other SF1 helicases.

从胺基酸序列分析显示，竹嵌纹病毒复制酵素中间区域的类解螺旋酵素具有解螺旋酵素的保留性胺基酸区域，经实验显示此蛋白具有ATPase和RNA 5'-triphosphatase活性，而且是依赖镁离子进行反应的，其中保留性胺基酸的突变会破坏其催化能力，说明这在大肠杆菌表达的蛋白质经过变性及重新摺叠的过程，具有和其他SF 1解螺旋酵素相似的特性。

The signal peptides of chicken Igλ light chain and murine plasmingen were fused to the GFP N terminus using site-directed mutagenesis to construct the vectors with signal sequence, pEGFP-SPc and pEGFP-SPm. The chicken Igλ gene was inserted into the two vectors with signal sequence after double enzyme cutting.

分别将鸡Igλ轻链信号肽、小鼠纤溶酶原信号肽与pEGFP-C1载体的绿色荧光蛋白N端融合，产生带有信号肽的中间载体pEGFP-SPc和pEGFP-SPm。

An astaxanthin overproducing strain was obtained by mutagenesis. in 200l common stirring fomenter, 60mg/l - 70mg/l astaxanthin were harvested by fed-batch cultivation of phaffia rhodozyma. results of optimization of extract condition indicated astaxanthin productivity was 1.14mg/g by acid methods under optimal condition. this achievement reached to the leading level of the field in china, and also advanced method internationally. in the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.

分离选育出虾青素高产的海洋红酵母，在200l中试罐中发酵虾青素，虾青素的产率达60-75mg/l发酵液；对天然虾青素的无毒提取技术进行研究，虾青素的提取得率为1.14 mg/g干菌体；虾青素发酵水平和提取技术水平为国际先进，国内领先，为用发酵法生产天然虾青素打下坚实的基础。

The strategies of large-scale mutagenesis and gene screen include chemical mutagenesis, insertional mutagenesis and gene trap.

用于大规模基因突变与筛选的主要策略有化学诱变、插入突变、基因诱捕。

The strategis of large-scale mutagenesis and gene screening include chemical mutagenesis,insertional mutagenesis and gene trap.

用于大规模基因突变与筛选的主要策略有化学诱变、插入突变、基因诱捕。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。