查询词典 mut

The single-chip and high integration, processing capability, the system of simple structure, low cost, small size, etc., so a single-chip micro-printer control technology has been applied in many fields, this paper, the application of EL-MUT - 111 single-chip microcomputer / microprocessor systems and experiment with the printer 8086CPU parallel communication methods to achieve control over their design and printing method.

而单片机又具有集成度高、处理能力强、系统结构简单、价格低廉、体积小等优点，所以以单片机控制微型打印机的技术已经在许多领域得以应用，本文研究应用EL-MUT-111单片机/微机实验系统及8086CPU通过与打印机并行通信方法来控制其实现打印功能的设计。

To find out the difference between Mut+ and Muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions.

在相同的培养条件下比较了Mut~+和Mut~s重组菌株表达PAP的异同。

SDS-PAGE results showed that as to Mut+ recombinant highest yield was obtained after 4 days inducing and with the culturetime prolonged it reduced. Pokeweed antiviral protein gene expressed well when methanol concentration reached 10g/L. Pokeweed antiviral protein obtained high yield in thin acidic culture medium (pH6.0-6.4) and its quantity in total mass of secrete protein exceeded 30%.

SDS-PAGE分析结果表明，Mut~+组菌株在甲醇诱导第四天后PAP在培养液中积累量达到最高水平，延长培养时间会导致产量下降；在10g/L的甲醇浓度诱导下，PAP的表达量达到最高；培养基pH值在偏酸性条件下(6.0-6.4)PAP的表达量都维持在较高的水平。

MMAA, MMAB, and MUT are the three genes known to be associated with isolated methylmalonic acidemia.

MMAA，、MMAB和MUT是已知的三个与独立的甲基丙二酸血症相关的基因。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

Methods Amplify IL-6 (23)-PE40 gene from the previously constructed recombinant plasmid pKK-IL-6(23)-PE40 by PCR, and insert to the SnaB Ⅰand NotⅠ sites of vector pPIC9. Transform the constructed recombinant plasmid pPIC0-SN to P. pastoris GS115 by electroporation, screen Mut-recombinants for expression under induction of methanol.

采用PCR技术，将目的基因IL-6(23)-PE40插入到pPIC9载体中SnaBⅠ与NotⅠ位点，电转化至巴斯德毕赤酵母GS115中，筛选Mut-型重组酵母；甲醇诱导表达，体外细胞试验检测其细胞毒性。

All Mut+and Muts colones were induced respectively with methanol to secrete interesting peptide in shake-flask cultures for four days. After dialysis and lyophilization, the supernate of cultures were identified by SDS-PAGE and Western blotting to find the genetically engineered Pichia pastoris which expressed interesting peptide.

在摇瓶中分别用甲醇诱导Mut~+和Mut~s型转化子表达蛋白4d，取培养产物冻干浓缩，进行SDS-PAGE和Western blotting，分析蛋白的含量及纯度，筛选出能特异表达目的蛋白的基因工程菌。

The mutation detection rate in individuals known to have mutations in MUT through complementation studies is not yet known.

利用互补分析获得的已知带有MUT基因突变的个体的突变检测率未知。

PCR was used to confirm the insertion of HDLR gene into the genome of Mut+ transformants.

应用PCR方法确定目的基因的整合和Mut+表型。

In one embodiment, a MUT on a substrate includes an acoustic cavity formed within the substrate at a location below the MUT.

在一种实施形式中，基片上的MUT包括形成于基片内而在MUT之下一个位置的声腔。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。