查询词典 muscle cell

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

BACKGROUND: Studies concerning muscle-derived stem cell transplantation mainly focused on treatment of muscular dystrophy. There are no studies on muscle-derived stem cells for treatment of denervated amyotrophy combined therapy.

背景：目前，国内外有关肌源性干细胞移植的研究主要集中在治疗肌营养不良性萎缩症方面，尚未见涉及将肌源性干细胞应用于失神经肌萎缩综合治疗的报道。

Part three: An experiment study on the repair of peripheral nerve injury by NT-3 gene modified neural stem cells objective To investigate the effective treatment of peripheral nerve injury with NT-3 gene modified neural stem cells, to explore the feasibility of the treatment by means of gene transfection Methods 54 SD rats were divided evenly into 3 groups. The sciatic nerve transected model were made and sutured end to end with epineurium. The neural stem cell without NT-3 gene or with NT-3 gene modified were injected into triceps muscle one time a week, saline injected as control group. The nerve trunks of stomas and triceps muscle were acted as specimen three weeks、six weeks and nine weeks after operation.

最后，为了探讨神经营养素-3基因修饰的神经干细胞对周围神经再生修复的影响，从转基因角度探讨治疗周围神经损伤的有效方法，我们选用54只SD大鼠随机分为3组，造成坐骨神经切断损伤模型，神经外膜端端对位缝合后，于小腿三头肌每周分别注射生理盐水、未被神经营养素-3基因修饰的神经干细胞、及神经营养素-3基因修饰的神经干细胞。

Result: At two weeks after OVA challenge, in model group airway wall thickness and positive cell nucleus of airway smooth muscle begin to grow up. At four weeks after challenge, airway smooth muscle content begins to elevate. At two and four weeks, expression of phenotype mark protein begins to low down, except for sm α-actin at two weeks.

结果发现，模型组大鼠激发二周后出现管壁增厚，四周后出现平滑肌层增厚，二周时即开始出现气道平滑肌Brdu掺入增多；smα-actin于二周时表达增强，而于四周时减弱，sm-MHC、calponin在二周、四周时表达均出现减少；电镜发现平滑肌细胞肌丝稍减少，而高尔基体、粗面内质网等细胞器增多。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分，减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常，使多种脏器组织的细胞浆内核糖体丰富，线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

The first, many tuberculosises in glandular stomach were found besides liver cell degeneration, pneumoncongestion, epithelium cell degeneration of tubuli tenales, necrosis and kidney hemorrhage, cardiac muscle celldegeneration, heart hemorrhage, cerebrum hemorrhage, satellitosis and neuronophagia, cerebellum purkinje cell necrosis.

发现病例1，肝细胞变性，肺淤血，肾小管上皮细胞变性、坏死，间质出血，心肌纤维变性，间质出血，大脑出血，&卫星化&与&噬神经&现象，小脑浦肯野氏细胞坏死，还可见腺胃有大量的结核结节。

To study the effects of skeletal muscle satellite cell on muscle atrophy so as to provide the experimental data for treating denervated muscle atrophy.

研究肌卫星细胞对萎缩肌肉的作用，为临床提高治疗失神经肌萎缩的疗效提供实验依据。

Plenty and purified satellite cells are obtained with these methods.IGF-1 could promote the proliferation and differentiation of mouse skeletal muscle satellite cell. Adequate single satellite cell could be obtained by frequent passaging method and the preparation are ready for for satellite cell transplanting.

结果：此方法可以获得足量、纯度较高的肌卫星细胞，适宜浓度的IGF对肌卫星细胞有较强的促增殖及促分化作用，频繁传代可以获取足量的单个肌卫星细胞，为肌卫星细胞移植做好了前期准备。

We employed a cell line of mouse podocytes immortalized with the simian virus 40 large tumor antigen gene, and examined the cell proliferation using colony-forming and MTT assays following treatment with various concentrations of bFGF. BMP-7, alpha-smooth muscle actin e podocytes were detected by western blot. Methyl blue staining was used for the observation of cell morphology.

利用SV40肿瘤抗原转染所获得的永生性小鼠足细胞系培养，以集落形成法和噻唑蓝MTT还原实验测定不同浓度bFGF刺激下的足细胞增殖，采用SDS-聚丙烯酰胺凝胶电泳和Western blotting 检测BMP-7、alpha-平滑肌肌动蛋白、以及波形蛋白的蛋白质水平表达，同时以甲基兰染色观察细胞形态。

To study the effects on the body of animals after incorporation of 3H-TdR and its mechanism, the laws of distribution A retention and absorbed dose in blood , thymus, spleen, femur, heart, lung,kidney, liver, brain , spermary , small intestine and muscle were detected by liquid scintillation counter after 3H-TdR was injected into the tail vein of mouse; the process of dynamic incorporation of in the lymphocytic cell nucleus of blood , thymus , spleen and the nucleus of femur was monitored; the absorbed dose in the cell nucleus was estimated; the influence to RNA"s synthesizing and cell"s survival was observed using the technology of CLSM; A segment of Egr-1 gene was cloned with reverse transcriptase- polymerase chained reaction, the influence to the expression of Egr-1 gene in the spleen cells was observed.

为了探讨有机结合氚内污染对生物机体的影响及其作用机理，本课题应用均相液体闪烁测量技术研究了氚标记胸腺嘧啶核苷(~3H-TdR)经尾静脉注入小鼠机体后，在血液、脑、胸腺、心脏、肺脏、肝脏、脾脏、肾脏、小肠、睾丸、肌肉和股骨共12种组织器官的生物动力学分布规律及其吸收剂量；应用纸片法液体闪烁测量技术研究了~3H-TdR内污染在外周血、胸腺和脾脏淋巴细胞及骨髓细胞的动态滞留规律，估算了细胞核的吸收剂量；应用共聚焦显微镜观察了~3H-TdR内污染对脾细胞核酸合成及其存活率的作用，应用反转录PCR技术研究了~3H-TdR内污染对脾细胞早期生长反应基因-1(Egr-1)转录表达的影响。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。