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To ealuate the engraftment potential of SSCALDH cells in io, we transplanted them into mouse brain.

为了评估体内SSCALDH细胞的移植物移入潜能,我们将这种细胞移植入小鼠脑中。

10% chloral hydrate anesthesia and glass cannula enswathement are availability for mouse tail skin transplatation. The failure rate of this method is low. It is suitable for students experiment.

10%水合氯醛麻醉结合玻璃套管法包扎进行小鼠尾部皮肤移植是一种有效、简便、经济的小鼠尾部皮肤移植手术实验方法;此方法技术失败率低,适合学生实验课采用。

The recombinant mouse enterokinase light chain could be well expressed in Ecoli, but most of them were inclusion.

利用pET32a/BL21(DE3)表达系统成功地在大肠杆菌中表达了重组鼠源性肠激酶轻链,表达量约占大肠杆菌BL21(DE3)总蛋白的30%,但多以包涵体的形式存在。

Objective To establish a prokaryotic expression system for expressing mouse enterokinase which could recognize and cut the sequence of4-Lys in order to provide a technique platform for application of recombinant enterokinase.

目的:制备能在原核表达系统中表达并特异性识别4-Lys序列的鼠源性肠激酶,为推广应用重组肠激酶提供技术平台。

Recombinant mouse enterokinase can be expressed in Ecoli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达,天然构象重组肠激酶的获得还需对表达系统进行优化。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA,将其克隆入pET32a原核表达载体中,并在大肠杆菌BL21(DE3)中进行表达,然后以镍亲和层析法对表达产物进行纯化。

In mouse, the position of first polar body (PB1) and MⅡ nucleus or spindle is moveable and thus it is difficult to enucleate beneath the PB1. In this study, the 3% sucrose treatment was prepared for enucleation.

在小鼠的卵母细胞中,第一极体(PB1)与MⅡ期核或纺缍体的位置是不固定的,因此导致了去核的困难。

The stereocilia of OHCs are extremely short. We can observe thephenomena of rupture and inosculation of stereocilia, which is alike senile deafness ofICR mouse. The OHCs bodies of bats have two leading characteristics. Firstly, theOHCs bodies are obviously short. The Deiters cell phalanges are of exaggerated, andthe cup formation of Deiters cell body enwrap the bottom of the OHCs.

蝙蝠耳蜗外毛细胞体,主要特征有两点:其一是细胞体明显短,三排Deiters细胞的指突明显与顶表面相连,而且毛细胞体基本被Deiters细胞的杯状膜包裹;其二是外毛细胞体在基底部呈烧瓶状,并不是一般哺乳类动物的试管样形态。

Here we show reprogramming of advanced epiblast cells from embryonic day 5.5–7.5 mouse embryos with uniform expression of N-cadherin and inactive X chromosome to ES-cell-like cells in response to LIF–STAT3 signalling.

李喜和与剑桥大学合作开展的这项研究,首先是对着床后5.5—7.5天小鼠外胚层细胞诱导培养过程中关于LIF因子作用的重新认识。

Objective To evaluate the significance of mouse anti-human monoclonal antibody LH 7∶2 in the diagnosis of distrophic epidermolysis bullosa.

目的 探讨鼠抗人单克隆抗体LH 7∶2诊断营养不良型大疱性表皮松解症的意义。

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