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Mouse fetal tissues could be cut into uniform small pieces by scissors cutting and needle grinding, which grew well in tissue culture and great amounts of cells grew from the attached small tissue clump to form primary mouse embryonic fibroblasts monolayer.

将这些组织块培养48h,大量的梭形细胞从贴壁小组织块的边缘游离出的,细胞密度大,细胞生长旺盛,很快形成原代小鼠胎儿成纤维细胞单层。

It is to use a high degree of specificity of the antibody immune response and chromatography analysis technology, marked by colloidal gold β-HCG and mouse monoclonal antibody IgG and fixed in the nitro-cellulose membrane of the anti-α-HCG and sheep anti-mouse antibody IgG Antibody, the specificity of detection in urine HCG.

是利用高度特异性的抗原抗体反应及免疫层析分析技术,通过胶体金标记的β-HCG单克隆抗体和鼠IgG以及固定于硝基纤维素膜上的抗α-HCG抗体及羊抗鼠IgG抗体,特异性的检测尿液中HCG。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞,CM联合20%FBS诱导骨髓间充质干细胞,同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组,分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化,并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化,用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

In appearance, the appearance of buildings using wireless mouse SG938 paint surface treatment process, smooth and delicate, making it more than an ordinary mouse easy to clean and smooth feel.

在外观上,国宇SG938无线鼠标外表采用烤漆工艺表面处理,光滑细腻,这使它比普通的鼠标更容易擦干净而且手感顺滑。

In this study, in utero transfection plasmid DNA method was set up to embryonic mouse. The GFP expression vector pCAGGS-IRES-EGFP was microinjected into the lateral cerebral ventricle of E 14.5d mouse embryos through the uterine wall. Then, the 30 V square-wave pulse was delivered across the head for 5 times and interval 50m sec by electrodes in parallel with the median raphes.

本研究旨在通过子宫内电穿孔法,建立鼠胚大脑皮层体内基因转移体系:将带有绿色荧光蛋白的表达载体pCAGGS-IRES-EGFP显微注入胚胎期14.5 d的鼠胚侧脑室中,电极沿与脑中缝平行的方向夹持鼠脑,在电压30 V,脉冲时间50 ms,脉冲数5的条件下方波电击,将外源基因转移到室管膜层细胞。

In 3 of 14 mice surviving long-term transplanted with bone marrow cells transduced with high-titer virus, bone marrow, spleen and thymus from two mice and bone marrow and spleen from another mouse contained the intact proviral genome. Long-term expression of the transferred gene was seen in one mouse at level of 7%of endogenous murine α-globin gene.

5%。在2只长期造血重建小鼠的骨髓、脾、胸腺及另一只小鼠的骨髓及脾中整合有人β-珠蛋白基因,在一只重建小鼠的骨髓和脾中有人β-珠蛋白基因表达,约为鼠α-珠蛋白基因的7 %。

This study was conducted to clone the sheepβ-lactoglobulin gene and the human myelomonocytic surface differentiation antigen CD14 gene (hCD14) by PCR, and this two gene were fused to construct oBLG-hCD14 fuse gene, this fuse gene were microinjected into pronuclei of mouse eggs to produce transgenic mouse.

本研究拟利用PCR和分子克隆技术,首先获得绵羊β-乳球蛋白基因调控序列片段和人CD14基因(hCD14)全程编码序列,并构建oBLG-hCD14融合基因,最后利用微量注射法将融合基因导入小鼠受精卵以产生转基因小鼠。

Finally, we'll also want to get the logical coordinates of the mouse pointer, and we do this with the ElementToLogicalPoint API call, passing the current mouse position (which can be de-rived from the MouseEventArgs object that is passed to this function) and then loading the re-sults into the pLast variable.

最后,我们还需要获取到当前鼠标所在的逻辑坐标值,我们可以通过ElementToLogicalPoint方法来获取,传递当前鼠标的位置到该方法(鼠标的当前位置可以通过MouseEventArgs对象参数来获取),然后将返回结果保存到PLast变量中。

I also thought that the beginning of the phenomenon of the failure was due to display card driver caused the destruction, so I right-click the desktop system, the "My Computer" icon on the shortcut menu from the pop-up the implementation of "property "command, open the System Properties settings window, click the window of the" hardware "tab, and in the corresponding tab page, click" Device Manager "button, open the device list window, use the mouse to start the window "Show card" option, and then double-click the mouse "standard VGA graphics adapter" project, open the corresponding project page the driver tab, click the tab page in the "Uninstall" button to display the current card driver procedure removed, and then restart the computer system, then let the system automatically re-installed the graphics card driver.

开始阶段笔者还以为该故障现象是由于显示卡驱动程序遭到破坏引起的,于是笔者用鼠标右键单击系统桌面中的&我的电脑&图标,从弹出的快捷菜单中执行&属性&命令,打开系统的属性设置窗口,单击该窗口中的&硬件&标签,并在对应的标签页面中单击&设备管理器&按钮,打开系统的设备列表窗口,用鼠标展开该窗口中的&显示卡&选项,再用鼠标双击&标准 VGA 图形适配器&项目,打开对应项目的驱动程序标签页面,单击该标签页面中的&卸载&按钮,来将当前的显示卡驱动程序删除掉,之后将计算机系统重新启动了一下,同时让系统自动重新安装了显示卡驱动程序。

With Mouse Wheel Control, you can use the mouse wheel even where it is not supported, change its functionality and extend its use.

与鼠标滚轮的控制,您可以使用鼠标滚轮,即使不支持它,改变它的功能和扩大其使用。

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