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Methods M. penetrans was cultured and its LAMPs were extracted. Mouse macrophages were stimulated with M. penetrans LAMPs to analyze the production of nitric oxide. Cell prolification was detected in M. penetrans LAMPs-stimulated mouse macrophages by MTT assay.

用SP-4培养基培养穿透支原体并提取其脂质相关膜蛋白,用提取的脂质相关膜蛋白刺激小鼠巨噬细胞,以ELISA法检测NO的产生,并用MTT法检测其对小鼠巨噬细胞增殖的影响。

The main objective of the dissertation was to improve and modify the experimental procedures of mouse nuclear transfer and pronuclear transplantation, respectively, and try to establish new possible methods for mouse cloning and pronuclear transplantation.

本论文的主要目的是改进和修改小鼠核移植和原核置换的实验程序,试图建立新的小鼠核移植和原核置换的可行方法。

To optimize the in vitro culture system of mouse pronuclear zygote and to investigate the effect of FBS, PVA, hLIF on mouse zygote development in vitro, we did four experiments separately.

筛选最佳的小鼠胚胎体外发育培养体系,并进一步探讨不同浓度的FBS、PVA、hLIF对其体外发育的影响,从而为小鼠卵母细胞核移植后重构胚的体外培养提供支持。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本实验主要利用卵母细胞的乙醇激活、胚胎体外培养和免疫荧光细胞化学方法对小鼠卵母细胞乙醇激活效率、影响因素、孤雌胚类型、发育、原核形成、迁移及早期胚胎卵裂过程中细胞骨架的组装、作用等进行了系统的研究和分析,结果显示:(1)小鼠卵母细胞的乙醇激活结果表明,随着乙醇浓度的升高和作用时间的延长,卵母细胞激活率和发育率都显著提高,但乙醇浓度过高和作用时间过长会导致卵母细胞碎裂率的显著增加,7%乙醇作用卵母细胞7min 为最佳激活条件。

DNA was microinjected into male pronuclei of fertilized mouse eggs,the injected mouse eggs were then transplanted into the oviduct of pseudopregnant female mice.

摘 要:目的建立人纤溶酶原激活物抑制物1(PAI-1)转基因小鼠。方法利用构建的高效真核表达载体pCAGGS-PAI-1,制备注射用DNA。

Senocular developed a virtual mouse procedure, you can control the number of column control the mouse controls the movement and action.

Senocular 开发了一个虚拟鼠标的程序,你可以通过控制栏中的一些控件控制鼠标的移动和动作。

there are infection on some seroenzyme activity of the p21(superscript HBsAg/HbsAg) and p21(superscript HBX/HBX) was introduced into p21 locus of the mouse. The activities of the seroenzymes have been changed due to the p21(superscript HBsAg/HbsAg) and p21(superscript HBX/HBX) was introduced into p21 locus of the mouse.

p21(上标 HBsAg/HBsAg)和p21(上标 HBsAg/HBsAg)转基因小鼠的血清酶在不同的年龄具有一定的变化规律,说明p21(上标 HBsAg/HBsAg)和p21(上标 HBsAg/HBsAg)转基因小鼠的血清酶具有一定的特征,为其功能性研究及临床检验具有重要参考价值。

The study was performed in a somatically activatable mutant KRAS -driven model of mouse lung cancer, which the researchers note is the first mouse model of squamous-cell carcinoma."All other murine genetic models of lung cancer of which I am aware are adenocarcinomas, yet squamous is the most common histology worldwide," Dr. Sharpless commented.

Sharpless医师建议,本研究以老鼠肺癌之身体活化突变导引模式进行,研究者指出这是第一个鳞状细胞癌的老鼠模式,其它所有的老鼠基因肺癌模式大多是腺癌,但是鳞状细胞癌是全球最常见的病理。

The study was performed in a somatically activatable mutant KRAS -driven model of mouse lung cancer, which the researchers note is the first mouse model of squamous-cell carcinoma."All other murine genetic models of lung cancer of which I am aware are adenocarcinomas, yet squamous is the most common histology worldwide," Dr. Sharpless commented.

Sharpless医师建议,本研究以老鼠肺癌之身体活化突变导引模式进行,研究者指出这是第一个鳞状细胞癌的老鼠模式,其他所有的老鼠基因肺癌模式大多是腺癌,但是鳞状细胞癌是全球最常见的病理。

To establish a mouse microinsemination model, Intracytoplasmic sperm injection and subzonal sperm injection were studied use mouse gamets.

我们用小鼠卵细胞胞质内精子注射和透明带下精子注射的方法,对显微授精过程进行了初步探讨,对配子的采集和准备、精子的优化及显微注射的操作等作了阐述。

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