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Results After seven days of culture,some BMSCs in grupe A appeared typical morphological neuronal traits.Cell bodies appeared refractile,conelike or spherical with long processes,NSE was expressed in group A.

结果A组诱导7d后有部分细胞具备神经元样细胞形态，胞体呈锥形或圆形，有较长单极或多极的突起，有NSE阳性细胞表达。

When cells undergo programmed cell death, cell corpses adopt a refractile and disc like structure. Using this morphological change as a cell death marker, we analyzed the death of the first 13 cells in the AB cell lineage of wild type and cnx-1; crt-1 mutant embryos.

进一步以4D摄影分析线虫AB世系细胞中最早死亡的13颗细胞之相对死亡时间和细胞尸体持续的时间长短的结果则显示，此死亡细胞数量提高的现象应是源於吞噬步骤发生问题而非细胞死亡执行上的问题。

Results After seven days of culture, some BMSCs appeared typical morphological neuronal traits. Cell bodies appeared refractile, cone-like or spherical with long processes, NSE was expressed. Animals in group A and group B recovered their motor functions in a varing degree, the difference of BBB score was significant (P.05). Hematoxylin and eosin staining demonstrated that the lesion areas of the spinal cord in group A recovered better.

结果 诱导7d后有部分细胞具备神经元样细胞形态，胞体呈锥形或圆形，有较长单极或多极的突起，有NSE阳性细胞表达；细胞移植后1~5周，A、B组动物运动功能均有不同程度恢复，但B组恢复较慢，BBB评分差异有统计学意义(P.05)；组织形态学观察示A组损伤脊髓恢复较好。

Results After seven days of culture,some BMSCs appeared typical morphological neuronal traits.Cell bodies appeared refractile,conelike or spherical with long processes,NSE was expressed.Animals in group A and group B recovered their motor functions in a varing degree,the difference of BBB score was significant(P.05).Hematoxylin and eosin staining demonstrated that the lesion areas of the spinal cord in group A recovered better.

结果 诱导7 d后有部分细胞具备神经元样细胞形态，胞体呈锥形或圆形，有较长单极或多极的突起，有NSE阳性细胞表达；细胞移植后1～5周，A、B组动物运动功能均有不同程度恢复，但B组恢复较慢，BBB评分差异有统计学意义(P.05)；组织形态学观察示A组损伤脊髓恢复较好。

ResultsAfter seven days of culture, some BMSCs appeared typical morphological neuronal traits: the refractile and cone like or spherical cell bodies and long processes, expressed Nestin or NSE or GFAP.

结果 实验组诱导7 d后有部分细胞具备神经元样细胞形态，胞体锥形或圆形，有较长单极或多极的突起，均有Nestin、NSE、GFAP阳性细胞表达，且3种细胞数量差别有显著性（t=3.266~6.099，P.05）。

ResultsAfter seven days of culture, some BMSCs appeared typical morphological neuronal traits: the refractile and conelike or spherical cell bodies and long processes, expressed Nestin or NSE or GFAP.

结果 实验组诱导7 d后有部分细胞具备神经元样细胞形态，胞体锥形或圆形，有较长单极或多极的突起，均有Nestin、NSE、GFAP阳性细胞表达，且3种细胞数量差别有显著性（t=3.266~6.099，P.05）。

And whereas species were originally defined in terms of morphological traits, today biologists generally use the criterion of a reproductively

来自于生物化学和比较解剖学等亚生物学领域的证据有助于划分物种和更高级的分类单位，然而物种最初依据形态学特征进行分类的，今天生物学家大体上使用孤立多产的群体作为标准。

In general, species are regarded as distinct if they remain reproductively isolated for most of their geographical range and have recognizably distinct morphological characteristics.

总而言之，物种被认为是在其大部分生态位内独立进行繁育并具有显著形态学特征。

Different types of LIDH were not only exhibit morphological differences,they were also different histologically.The higher capillary infiltration rate of the extruded LIDH might be responsible fot its resorptive process and severe clinical findings.

腰椎间盘突出的不同病理类型不仅是形态学上的差异，也具有各自不同的组织学基础，破裂突出组织的较高血管浸润率很可能与其较严重的临床表现以及较明显的组织吸收过程相关联。

Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.

目的：探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率，分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响；方法：69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究；并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变；分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响；结果：15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生，所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁；12例PV、4例ET、4例IMF中检测到JAK2V617F；有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L，比较不同纤维增生程度的PV、ET患者的白细胞数均值，P值为0.367。

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