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RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

It was verified that the particle size of more than 90% of the acidic phosphorite powder was less than 4.227 μm, and more than 90% of activated phosphorite powder was less than 220 nm.

采用扫描电镜观察、X－射线衍射分析、激光粒度测试，证明了90%的酸化磷矿粉粒径为4.227 μm，90%的活化磷矿粉粒径达到220 nm。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中，地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长；实验组创面愈合时间较对照组提前了4天；第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多，上皮细胞分裂象也高于对照组，第4天的间皮细胞分裂象也高于对照组；自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

Results:(1) The mean BMI of patients except for women aged from 20 to 29 years, was much higher than that of the general population (P.01).(2) The overweight (BMI≥24) ratio of the patients was much higher than that of the general population (P.01).(3) Patients with hypernormal BMI were more than those with normal BMI (BMI4) except for patients aged from 20 to 29 years.(4) Patients with hypernormal BMI were significantly more than those with normal body weight in all other occupations except for young students.

结果：①除了女性20岁～29岁年龄段外，其余各组患者体重指数的均数均明显大于中国普通人群体重指数的均数（P均＜0.01）；②本组患者中男、女性体重指数超常者(BMI≥24)所占的比重，均明显大于中国普通人群中体重指数超常者所占的比重（P均＜0.01）；③除了男、女20岁～29岁年龄段以外，其余各年龄段的患者中体重指数超常者所占的比重，明显大于体重指数正常者(BMI4)所占的比重；④除青年学生外，其他职业中体重指数超常者所占的比重，明显超过体重指数正常者所占的比重。

Article 88 Where a fund manager or a fund custodian, in violation of the provisions of this Law, fails to place the assets of a fund under separate management or custody in separate accounts or misappropriates the fund assets, it shall be instructed to rectify and shall be fined not less than 50,000 yuan but not more than 500,000 yuan Where losses are caused to the fund assets or to the holders of fund units, it shall, in accordance with law, bear the responsibility to pay compensation The persons who are directly in charge and other persons who are directly responsible shall be given a discipline warning, or be suspended or disqualified from engaging in the fund business and, in addition, be fined not less than 30,000 yuan but not more than 300,000 yuan If the violation constitutes a crime, it shall be investigated for criminal responsibility in accordance with law

第八十八条基金管理人、基金托管人违反本法规定，未对基金财产实行分别管理或者分账保管，或者将基金财产挪作他用的，责令改正，处五万元以上五十万元以下罚款；给基金财产或者基金份额持有人造成损害的，依法承担赔偿责任；对直接负责的主管人员和其他直接责任人员给予警告，暂停或者取消基金从业资格，并处三万元以上三十万元以下罚款；构成犯罪的，依法追究刑事责任

It is concluded that bone marrow inhibition of hal group is more severe than that of nhal group. in aml,monocytic leukemia is easier to become into hal than other leukemias. in all,t-lineage antigens of hal group are more easily expressed than those of nhal group; the leukemia cells of hal group are naiver than those of nhal group,meanwhile the prognosis of hal is poor.

hal比nhal骨髓受抑程度更重，aml中单核细胞性白血病发生高白细胞性白血病的可能性高，all中hal比nhal更易于表达t系抗原，hal的白血病细胞较nhal处于更早分化阶段，同时hal预后不佳。

My Book prices rose more than a decade more than 20 times, and now each of the average price of the book than 30 yuan, much higher than the income level of residents.

我国图书价格上涨了超过10年的20多倍，而现在每个的平均价格预订超过30亿元，远远高于收入水平的居民。

Mean daily consumption of alcohol per day was greater in men than in women (28.2 25.0 g vs 13.9 15.0 g), as reflected in both in the lowest (mean, 4.1 g vs 1.1 g) and highest (mean, 68.7 g vs 38.8 g) quintiles of daily consumption. More than 50% of women consumed less than 10 g a day of alcohol, and more women were abstainers than men (3.0% vs 2.1%).

男性的平均酒精日摄取量要比女性高28.2 ( 25.0 g vs 13.9 ( 15.0 g)；最低(平均， 4.1g vs 1.1g)及最高(平均， 68.7g vs 38.8g)的日摄取量也是男性高於女性，超过10%的女性，日摄取量低於10 g；对酒精节制的女性人数也高於男性(3.0% vs 2.1%)。

Mean daily consumption of alcohol per day was greater in men than in women (28.2 25.0 g vs 13.9 15.0 g), as reflected in both in the lowest (mean, 4.1 g vs 1.1 g) and highest (mean, 68.7 g vs 38.8 g) quintiles of daily consumption. More than 50% of women consumed less than 10 g a day of alcohol, and more women were abstainers than men (3.0% vs 2.1%).

男性的平均酒精日摄取量要比女性高28.2 ( 25.0 g vs 13.9 ( 15.0 g)；最低(平均， 4.1g vs 1.1g)及最高(平均， 68.7g vs 38.8g)的日摄取量也是男性高于女性，超过10%的女性，日摄取量低于10 g；对酒精节制的女性人数也高于男性(3.0% vs 2.1%)。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。