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Jiangsu Province, the past couple of years through auction, tender average price of land for more than 600 yuan per square meter, more than an agreement to sell more than 2 times higher in some places even more than 10 times.

江苏省，在过去几年里，通过拍卖，招标的土地平均价格超过600元每平方米，超过了一项协议，销售超过2倍，在一些地方甚至超过10倍。

Those who smuggle, traffic, transport or manufacture opium with a quantity less than 1,000 grams but more than 200 grams, those who smuggle, traffic, transport or manufacture heroin or methylaniline with a quantity less than 50 grams but more than 10 grams, and those who deal with other drugs in large quantity are to be sentenced to more than seven years of fixed-term imprisonment and, in addition, be sentenced to a fine.

走私、贩卖、运输、制造鸦片二百克以上不满一千克、海洛因或者甲基苯丙胺十克以上不满五十克或者其他毒品数量较大的，处七年以上有期徒刑，并处罚金。

Now it preserves more than 1750 domestic and international bacterial microorganism, including more than 380 bacteria, more than 750 saccharomycete, more than 620 chitomycetes, roughly cover all kinds of bacterial microorganism in this field, among them some good microorganism's production reach the international high level.

目前该中心保藏国内外各类工业微生物菌种1750余株，包括细菌380余株，酵母菌750余株，丝状真菌620余株，基本覆盖了食品与发酵行业各类生产和科研用微生物，其中许多优良菌株的生产性能具有较高水平。

Hebei Sheng million Wire Mesh Fence Factory is a wire drawing, welding net, welding, cutting, press brake forming, IMPREGNATION finished installation, the production of service capabilities, large-scale specialized metal guardrail, Fence manufacturer Founded in May 2005, the Company specializes in highways and railways, fencing wire mesh, separation, isolation network, fencing, protective nets, iron railing, guardrail and other sports, the company currently employs more than 50, including engineers, a , design, accounting, five, more than 30 types of electric welder, a dozen of their own intellectual property rights, is the production of 15 major series, more than 90 models, blocking more than 500 kinds of care products, by polishing, rust, Straightening , welding, galvanizing, IMPREGNATION made with corrosion-resistant, anti-aging, acid, non-fading, surface smooth and bright, comfortable feel and so on.

护栏网厂是一家集拔丝、焊网、焊接、裁剪、折弯成型、浸塑成品安装，生产服务能力、规模较大的专业化金属护栏、隔离栅生产厂家，成立于2005年5月，本公司专业生产公路、铁路的护栏网、隔离栅、隔离网，防护栏，防护网、铁艺护栏、体育场护栏等，公司现有员工50多名，其中工程师一名、设计、会计五名，各类电气焊工30多名，拥有自主知识产权十余项，现生产15大系列、90多个型号、500多种护拦网产品，产品经抛光、除锈、调直、焊接、镀锌、浸塑而成，具有耐腐蚀、抗老化、耐酸碱、不褪色、表面平整、光亮、手感舒适等优点。

The company has fixed assets: the factory area of more than 12,000 square meters, building area of more than 8000 square meters; computer flat seam 300 cars, double needle car, five-line beat edge equipment such as vehicles, more than 60 Units; outstanding more than 350 employees; the annual output reaches 200 10000; annual sales of about 5,000 million.

公司拥有固定资产：厂区面积12000多平米，建筑面积8000多平米；电脑平缝车300台，双针车，五线拷边车等配套设备60余台；优秀员工350多人；年产量达200万件；年销售额约5000万左右。

The company's existing more than 300employees, more than 50 technicians, collect development, design, manufacture and sales in one of the modern enterprise, the company currently has two production bases, which are located in Lu-qiao-Huang-lang and Jiao-jiang-tai-he Industrial Zone ,covers area about 40,000 square meters, To achieve a die-casting, CNC machining, heat treatment processing, painting and assembling the five automatic production processOur company own CNC etc advanced production equipment;more than 99% self-made products; a full range of testing equipment and perfect modern management system so that the quality of the product to be a strong guarantee, the company has developed pneumatic wrench, pneumatic-grease pump and air hose reel etc more than 100 varieties products series, the product passed the CE certification, selling all over the world, our company enjoy a higher reputation at home and abroad

公司现有300多名员工，其中工程技术人员50多人，是集开发设计、制造销售于一体的现代化企业；目前公司拥有两个生产基地，分别位于椒江太和工业区和路桥黄琅工业区，占地面积4万平方米，实现了产品压铸、CNC加工、热处理加工、涂装、装配五大工艺自动化生产；拥有进口CNC数控等先进生产设备，产品自制率高达99%；齐全的检测设备和完善的现代化管理机制使产品的质量得到了有力的保证；公司已形成风扳、注油器、绕线器等系列100多个品种，产品通过了CE认证，畅销世界各地，在国内外同行中享有较高的声誉。

PUNCHING Shanghai Second Machine Co., Ltd., covers an area of more than 46,000 square meters, plant area of more than 18,000 square meters, more than 500 employees, more than 50 engineers, strong technical force, advanced processing equipment and scientific quality management, strong development capacity for the continuous development of our company has laid a good foundation, Shanghai Forging Machine Tool Co., Ltd. folding scissors purposes are: to use modern business management methods, in favor of the spirit of customer service on the principles of social production, sales, service .

上海冲剪二机有限公司，占地面积46000多平方米，厂房面积18000多平米，500多名员工，工程技术人员50多名，技术力量雄厚，加工设备先进，科学的质量管理，较强的开发能力为我公司的不断发展奠定了良好的基础，上海博锻剪折机床有限公司的宗旨是：用现代企业的管理方法，本着有利于客户，服务于社会的原则进行生产、销售、服务。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

The company occupies 60000 square meters fixed assets 42770000 yuan is mainly used in the production of large and medium-sized more than 100 sets of equipment for product quality testing equipment advanced more than 60 sets of Silver City has a trademark of boilers pressure vessels products 37 series 167 specifications models annual production capacity of more than 1000 up to the boiler steam tons a second-class pressure vessel more than 500 sets.

公司占地60000平方米，固定资产4277万元，用于生产性主要大、中型设备100多台套，用于产品质量检测的先进设备60多台套，拥有银城商标的锅炉、压力容器产品37个系列，167个规格型号，年生产能力可达锅炉1000多蒸吨，一、二类压力容器500多台套。

Company personnel, technical strength, more than 600 the number of existing staff, including various types of professional and technical personnel more than 150 people, has production facilities more than 150 Taiwan, which caused Germany thanks to joint blessing automatic air spinning machine 23 Taiwan. In 2005, the annual production of over 5,500 tons of cotton yarn OE net sales revenue of more than 7,000 million, payment of taxes over 200 million profit over 200 million.

公司人才、技术实力雄厚，现有员工人数600多人，其中各类专业技术人才150多人，拥有生产设备150多台，其中引起德国赐来福全自动接头气流纺纱机23台。2005年，全年生产OE纯棉纱5500多吨，销售收入达7000多万元，上缴税金200多万元，利润200多万元。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。