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Cloning and sequence analysis of germin gene from wheatGermin in wheat have an higher oxalate oxidase activity.

小麦germin基因的克隆与序列分析小麦germin具有较强的草酸氧化酶活性。

A pair of primers were designed according to the germin gf-2.8 gene sequence reported in GenBank.Germin gene was isolated from the genomic DNA of wheat "Xiangmail3" by PCR amplification.

根据GenBank中的germin gf-2.8同源序列设计引物,通过PCR扩增,从湘麦13基因组DNA中得到了长度为812bp的目的片段,将PCR产物克隆到pDive载体上并测序。

Full-length cDNA of Gibel carp pou2 gene was cloned from gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 hp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product.

用RACE-PCR方法从原肠期SMART文库中扩增到银鲫pou2基因的全长cDNA,其全长为2421bp,开放阅读框为1416bp,编码471个氨基酸,与斑马鱼pou2基因的氨基酸序列一致性高达91.0%。

These results suggested that L.esculentum and L.peruvinanum share the high degree of homoeology sequence.Identification of the authenticity of hybrids by GISH was very difficult in present.

说明了栽培番茄与秘鲁番茄之间存在着高度的同源性,应用该技术对栽培番茄与秘鲁番茄的杂交种进行真实性分析时存在很大的困难。

The 16S rDNA sequence of the 4 strains were aligned with the 16S rDNA sequences of other species in genus Burkholderia,and the polygenetic tree was produced by using a Clustal V and treeview software.The results showed that P.cocovenenans subsp.farinofermentans was in a high homology with Burkholderia gladioli and Burkholderia cocovenenans, and they formed an independent phylogenetic clustal of genus Burkholderia.Key words: P.cocovenenans subsp.farinofermentans,16S rDNA sequcence,phylogeny

树状图及不同菌种之间的同源性比值:椰酵假单胞菌(P.cocovenenans subsp.farinofermentans)与唐菖蒲伯克霍尔德氏菌、椰毒伯克霍尔德氏菌的亲缘关系非常近,与其它菌种的亲缘关系相对较远,椰酵假单胞菌、唐菖蒲伯克霍尔德氏菌和椰毒伯克霍尔德氏菌可归属于一个独立的系统发育系。

Taking for example the salt rhythms of the Qianjiang Formation in the Qianjiang Sag of the Jianghan Basin (which is a typical Paleogene saline lacustrine basin in eastern China), through a detailed study on cores, the authors have determined for the first time the Ⅳ-order salt rhythm based on Ⅰ-,Ⅱ-and Ⅲ-order salt rhythms which were determined previously and got to know that the sedimentary process following the sequence of desalinizing→salinizing and crystallization of salt minerals from halite rock→(mud-bearing) glauberite rock→dolomite-bearing mudstone (mud-bearing doloston)→mudstone→doloston→glauberite rock→halite rock. The authors also analyzed the Ⅳ-order salt rhythm and the correspondence between its sedimentary records and the fluctuation of the palaeosalinity of waters and the short-scale (0.05~1.0 ka duration) change of dry-moist palaeoclimate.

本文以我国东部独具特色的古近纪古盐湖盆地-江汉盆地的潜江凹陷潜江组盐韵律为例,通过对王平1、王云10-6、王80-2等3口井连续取心段的精细研究,在前人划分Ⅰ、Ⅱ、Ⅲ级盐韵律的基础上,首次划分出组成含盐层系基础韵律单元-Ⅳ级盐韵律,弄清了它的沉积过程基本遵循从石盐岩→钙芒硝岩→含白云石泥岩→泥岩→白云岩→钙芒硝岩→石盐岩的淡化-咸化序列和盐类矿物的析出顺序;解析了Ⅳ级盐韵律及其沉积组合记录与水体古盐度波动和短尺度(0.05~1.0 ka)古气候干湿变化之间的对应关系。

Under the fluoroscopic guidance, the glenohumeral joint was punctured, and 15-20ml of a mixed contrast solution (22%Omnipaque and 3mmol/L Magnevist) was injected inside the joint. Then SE T1-weighted sequence (repetition time/echo time=500 ms/20 ms) was performed in oblique coronal, oblique sagittal and axial plane within 45 minutes.

在透视下穿刺肩关节腔,注入15~20ml 22% Omnipaque和3mmol/L Magnevist的混合性造影剂,然后在45分钟内完成SE序列T1加权(TR/TE=500/20ms)的斜冠状面、斜矢状面和横断面MR成像。

Based on DNA sequence and the molecular mass of E.coR I and Pst Ifragments,the physical map of the gene encoding the A1aB1b-subunit of glycinin wasconstructed.

根据E.coR I、Pst I酶切片段及DNA序列分析结果,构建了大豆球蛋白A1aB1b亚基基因的物理图谱。

The result indicates the length of the clone sequence is 963bp and shares a similarity of 45.24 % with the published Glycine max glycinin subunit G7 (Gy7) gene promoter.but have more similar to quantity and distance of promoter elements, they all contained typical TATA-box, CAAT-box and necessary regulatory motifs of seed-specific expression.

测序结果表明所克隆的片段长为963bp,与预期设计的大小一致,该片段与已发表的11S球蛋白基因启动子序列的相似性为45.2%,但所含的启动子序列元件的数量和距离上很相似,均具有有典型的TATA盒和CAAT盒,以及种子特异表达所必须的调控元件。

The upstream regulatory region of the storage protein was separated from Glycine max variety "Ji Nong T7018" genomic DNA, according to published glycinin 11S G7 subunit (Gy7) gene(GenBank AF319776) sequence, a pair of primers was desgeined, in order to amplify the array before Gy7 gene initiation codon.

我们以大豆品种&吉农T7018&的基因组DNA为模板,根据已发表11S球蛋白G7亚基(Gy7)基因(GenBankAF319776)序列,设计一对引物,通过PCR扩增得到Gy7基因的启动子序列。

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