查询词典 monotonically decreasing sequence

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

Methods The yield of combinant human IL-18 expression in E.coli remains quite low because the sequence of mature hIL-18 has 37aa rare condons for E.coli in a total of 157aa.

它们的分子中没有N-糖基化位点，不存在二硫键，很可能巯基就是分子的活性中心。

Methods The yield of combinant human IL-18 expression in E.coli remains quite low because the sequence of mature hIL-18 has 37aa rare condons for E.coli in a total of 157aa.

设计特异性引物，以RT-PCR从外周血单个核细胞中扩增出人白细胞介素18成熟肽片段，克隆进入PBV220载体。

The data mapping modulation, by which the information data are mapped as the combinatorial sequence, and the security protocol method of the parallel combinatory SS communication system are presented in this paper.

本文提出了并行组合扩频通信的数据调制映射法，并以此为基础，给出了一种安全通信协议方式，既可作身份认证又有好的保密能力。

Obvious genetic divergence was observed between Nepal panda and those from Yunnan and Sichuan. Additionally, DNA sequence variations were also found in concolor gibbons.

此外，对来自云南和海南的黑冠长臂猿的线粒体DNA的序列分析证实了形态和地理分布上的亚种划分，并提示海南黑长臂猿可能是一个独立的种。

The floral morphogenesis of Coptis was observed under scanning electron microscopy. The initiation of all floral organs of the genus is spiral, the petal primordia are slightly retarded, the developmental sequence of the stamens is centripetal,the carpel primordia are conduplicate (i.e., horse-shoe shaped), the ovaries are half closed and the ovary stalk is formed in the developmental process.

本文运用扫锚电子显微镜观察了黄连属植物花的形态发生和发育过程，结果表明，该属植物所有的花部器官均为螺旋状发生，雄蕊为向心式发育，花瓣原基有微弱的延迟发育，心皮原基为对折型，子房为半封闭类型，子房柄是在发育过程中形成的。

The sequence of coned NGF gene was confirmed with the dideoxychain-termination method .

以双脱氧末端终止法对克隆的 NGFcDNA 进行测序。5。

Chapter Ⅲ is devoted to a comprehensive comparison and analysis of possessory lien with other confusable rights, namely, right of retention in respect of vessel, maritime lien, and also deals with the exertion sequence of these rights.

第三章对易与船舶留置权混淆的几种权利，如船舶扣押权、船舶滞留权、海上留置权等，进行了分析比较，并就船舶上可能同时存在的几种担保债权的受偿顺序进行了研究。

To identify whether our previous founding were exist in the series of our newly established cell cultures with the same genetic background and different metastatic potentials, and to make clear what are congenerous and various traits in the chromosomal or DMA sequence levels between these cells, in this study, a combination of conventional G-banding, comparative genomic hybridization, multiplex fluorescence in situ hybridization and arm or locus-specific fluorescence in situ hybridization were used to comprehensively characterize molecular cytogenetics aberrations of the above cell cultures.

为查明我所新建立的这些具有同一遗传背景而又在肝癌的转移潜能上存在明显差异的细胞是否也具有8p的缺失，并弄清它们在其它染色体及其区段的DNA水平上有着怎样的相似和不同，这些相似与不同又有着怎样的意义，本课题联合应用G显带技术、比较基因组杂交技术、多重荧光原位杂交技术和位点或臂特异荧光原位杂交技术，对上述细胞进行细胞及分子遗传学研究，以期了解其分子细胞遗传学异常的特征、找出转移相关的遗传学标记，为探讨肝癌转移机制提供材料。

The thesis brings forward Random Number Sequence Shuffling Technology in a creative way,it can reduces the Linear Congruential Generator pertinency and discrete grid phenomena prominently.

创造性地提出在遗传算法中随机数序列处理的新方法——随机数序列洗牌技术，有效克服了线性同余发生器产生的随机数序列相关与离散网格现象。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。