查询词典 monotonically decreasing sequence

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

A mutation type and a wideness type were established by clone and gene sequence detection.

基因测序技术对无缺失片段进行克隆、测序，筛选出突变型及野生型。

Sequencing and homology analysis revealed that the gene fragment was 395 bp in length and shared up to 89% similarity with NB gene from winter squash. The deduced amino acid sequence for the fragment was composed of 131 amino acid residues.

经过测序和同源性分析表明：该基因片段长度为395bp，与GenBank上所登录的笋瓜NR基因同源性达89%，根据所测序列推导该片段由131个氨基酸残基组成。

In the process of sequence matching the errors are accumulated. In order to eliminate them, the global error distributing method is proposed based on the wire cloth station foundation. In addition, in order to delete the redundant data and simplify model, the paper proposes the data integration method based on the deleting redundant data on the spatial division in the overlap region.

在序列拼接过程中会产生累积误差，为了消除累积误差，本文提出了基于闭合导线条件的整体误差分配计算方法；此外，为了减少冗余数据、精简建模数据，本文对于拼接后的满足精度的点云数据，提出了在重叠区域先进行空间划分再删除冗余点的数据融合方法并加以验证。

The chapters were then arranged according to their length and without regard to historical sequence.

章节安排，然后根据他们的长度和没有考虑到历史序列。

How's that for an un in tended con sequence of the women's liberation movement?

这难道就是妇女解放运动预期不到的结果吗？

Are there unique compositional techniques employed such as word painting, ostinato, melodic sequence, or sprechstimme, etc.?

有什么独特的作曲技巧被使用吗？诸如：生动描述、固定音型、旋律次序、诵唱声部等等？

A transaction is a logic unit of work, which is a sequence of SQL statement against database.

交易是一个逻辑单元的工作，这是一个序列的SQL语句对数据库。

Conventionally, computing the discrete Fourier transform that is equivalent to sampling of the z transform of the input sequence at equally spaced angles around the unit circle.

在以往，我们计算离算傅立叶转换相当於在z平面的单位圆上均匀的取样，然而，在某些应用上，非均匀的取样会更有效率。

The deduced amino-acid sequence of the TcGH had 210aa,including a putative signal peptide (22aa) located at its N-terminal. The homology of the TcGH amino-acid with relative species grass carp,black carp,bighead carp,carp,zebra fish,etc.

结果序列分析表明：唐鱼GH cDNA的5 非编码区为64 bp，3 非编码区为448 bp，开放阅读框633 bp，共编码210个氨基酸，包括22个氨基酸的信号肽和188个氨基酸的成熟肽。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。