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AIM: To examine the role of matrix metalloproteinases expressed by the tartrate-resistant acid phosphatase-positive mononuclear and multinucleated cells in articular cartilage damage.

目的：观察在胶原诱导的C57BL/6小鼠类风湿性关节炎模型中，基质金属蛋白酶-2、9在耐酒石酸盐酸性磷酸酶阳性的单个核及多核细胞中的表达及其在关节软骨损伤中的作用。

Extracellular particles, small mononuclear cells, red blood cells, and myoepithelial cells were markedly decreased in number.

胞外成分，小单核细胞，红细胞，肌上皮细胞数量上显著减少。

METHODS: Peripheral blood mononuclear cells from 33 AMI patients, 22 unstable angina patients and splenocytes from 35 AMI rats were collected. Cytokine-producing Th cells were monitored by 3-color flow cytometry after stimulated with phorbol myristate acetate and ionomycin.

采用流式细胞分析法对33例AMI病人，22例不稳定心绞痛病人和35例AMI大鼠进行Th细胞胞内干扰素γ和白介素4(IL-4)的动态监测，同时应用RT-PCR方法检测AMI大鼠心肌组织IFN-γ和IL-4以及T细胞表面趋化因子受体CCR3、CCR5和CXCR3 mRNA的表达。

The optimal yield of MAPCs was obtained when mononuclear cells were cultured at a initial plating density of 1 × 10~6 cells / cm~2 and the medium was exchanged to remove the nonadherent cells after 72 hours of inoculation.

结果表明，在低血清条件下，DMEM／F12培养基更适合于脐带血MAPC生长；1×10~6／cm~2是原代培养的适宜接种密度，原代第72小时首次换液为最佳换液时问。

Methods: Nonadherent cells from bone marrow or peripheral blood mononuclear cells of 8 patients with chronic myeloid leukemia were cultured with rhGM- CSF and rhIL- 4. The cytokines were supplemented every three days. RhTNF-α was used at the ninth day. All the cells were harvested at the twelfth day.

从8例慢性髓性白血病患者的骨髓或外周血中获取非贴壁细胞，利用细胞因子(rhGM蛳CSF、rhIL蛳4、rhTNF蛳α)联合培养，每3 d补充新的细胞因子，其中rhTNF蛳α为第9天添加，第12天收获细胞。

Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10％ heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法，分离32例乳腺癌患者外周血中的单个核细胞，在6孔培养板上(106/ml，2ml/孔)，用含10％热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient centrifugation. EGM-2 culture fluid was added, and then cells were plated on dishes coated with human fibronectin. After 48 h, the nonadherent cells were collected and replated on fibronectin-coated dishes.

采用Ficoll密度梯度离心法从人脐血中分离单个核细胞，加入EGM-2培养液，接种于包被人纤连蛋白的培养皿中贴壁培养，收集48h后的悬浮细胞重新贴壁培养至第7天，利用免疫荧光和流式细胞术鉴定。

METHODS: mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

METHODS: The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

METHODS: Bone marrow mononuclear cells were isolated from 5M4/M5 leukemia patients with high CD14 expression, and then divided into 3groups: adherent leukemia cells, nonadherent blasts, and total unfractioned blasts.

取5例初诊CD14高表达的M4或M5型白血病患者的骨髓标本，分离单个核细胞(bone marrow mononuclearcells，BMMNCs)，将白血病细胞分为3组：贴壁白血病细胞组、非贴壁白血病细胞组及总白血病细胞组。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。