查询词典 mononuclear cells

To isolate mononuclear cells from foetus umbilic cord blood, After MNCs were incubated in the presence of APS group (APS 200 mg·L-1) and control group for 24 h,the cells were stimulated with Epo（5 IU/mL） for 0,2,5,30 min; STAT5 was measured by ICC and laser confocal scanning microscope, JAK2、STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL in each group at different groups.

分离纯化胎儿脐带血单个核细胞，在常规体外培养体系加入APS（200 mg·L-1）作用细胞24 h，促红细胞生成素分别刺激0，2，5，30 min，免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达；Western-blotting-增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。

Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells.

对北京协和医院根据细菌学或／和组织学诊断的肺外结核患者31例，应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞。

MethodsThe frequency of CD4+CD25+T cells in the peripheral blood mononuclear cells from 30 cases of severe hepatitis B, 20 cases of chronic hepatitis B, 20 cases of chronic asymptomatic HBV carriers and 10 healthy controls were determined by flow cytometry and their serum HBV DNA levels were detected by Quantitative Fluorogenic PCR.

方法采用流式细胞仪检测30例重型乙型肝炎患者、20例慢性乙型肝炎患者、20例无症状乙肝病毒携带者和10例健康的外周血CD4+CD25+调节性T细胞（CD4+CD25+Treg）的水平，并应用荧光定量PCR方法测定上述研究对象血中HBV DNA滴度。

Methods The murine bone marrow mononuclear cells were induced into immatured dendritic cells with granulocyte-macrophage colony-stimulating factor and interleukin-4（IL-4）. The cell morphology was observed under an inverted phase contrast microscope, and the surface markers were detected by flow cytometry.

经粒-巨噬细胞集落刺激因子、白细胞介素-4（IL-4）诱导小鼠骨髓单个核细胞分化为未成熟树突状细胞，倒置相差显微镜下观察细胞形态，流式细胞仪检测细胞表面标志。

BACKGROUND: CD4+CD25+ T cells have low proliferation ability, and are about 4% of mononuclear cells in the peripheral blood.

背景：CD4+CD25+ T细胞增殖能力低，且在人外周血中仅占单个核细胞的4%左右。

Objective: To compare the functions of endothelial progenitor cells differentiated from cryopreserved and fresh bone marrow-derived mononuclear cells.

目的：对新鲜和超低温保存的骨髓来源单个核细胞体外扩增的内皮祖细胞进行功能比较。

An HIV-1 strain, SH01, isolated by coculture of peripheral blood mononuclear cells was easily passaged in MT4 cell line and multinuclear giant syncytia could be induced in MT2 cells.

用外周血单个核细胞注意混合培养法分离到的HIV-1 SH01株，具有典型的HIV颗粒的形态学特征，核心颗粒呈锥形，可见芽生释放的全过程。

Results: The TRAP positive multinuclear cells were induced from rat marrow mononuclear cells after inoculating for 5 clays. RT-PCR analysis showed that ASIC1, ASIC2 and ASIC3 mRNA were present in the osteoclasts, and the corresponding proteins expressions were detected.

结果：诱导5d后可见抗酒石酸盐酸性磷酸酶阳性多核细胞出现，大鼠破骨细胞在mRNA和蛋白水平均可检测到ASIC1、ASIC2和ASIC3基因的表达产物。

METHODS: Peripheral blood mononuclear cells from 33 AMI patients, 22 unstable angina patients and splenocytes from 35 AMI rats were collected. Cytokine-producing Th cells were monitored by 3-color flow cytometry after stimulated with phorbol myristate acetate and ionomycin.

采用流式细胞分析法对33例AMI病人，22例不稳定心绞痛病人和35例AMI大鼠进行Th细胞胞内干扰素γ和白介素4(IL-4)的动态监测，同时应用RT-PCR方法检测AMI大鼠心肌组织IFN-γ和IL-4以及T细胞表面趋化因子受体CCR3、CCR5和CXCR3 mRNA的表达。

Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10％ heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法，分离32例乳腺癌患者外周血中的单个核细胞，在6孔培养板上(106/ml，2ml/孔)，用含10％热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。