查询词典 mononuclear cells

Cytokine-induced killercells are a cluster of alloplasm cells,which are prepared from the mononuclear cells of human peripheral blood that might be stimulated by some kinds of cytokines.The proliferation and cytotoxicity of CIK cells are high,and the side effect is low.CIK cell is a more effective anti-tumor effector cell in adoptive immunotherapy.

细胞因子诱导的杀伤细胞是人外周血单个核细胞在体外经多种细胞因子刺激后获得的一群异质细胞，它具有增殖能力强、杀瘤活性高和杀瘤谱广、临床应用不良反应小的特点，是肿瘤过继免疫治疗中更为有效的杀瘤效应细胞。

Methods Peripheral blood mononuclear cells from healthy donors were induced to become dendric cells and CIK, using tumor antigen extracted from gastric cancer SGC7901 cells to sensitize DC, antigenpulsed dendritic cells were cocultured with CIK to generate CIK induced by gastric cancer antigenpulsed DC.

从正常人外周血单个核细胞诱导DC、CIK细胞，用胃癌细胞SGC7901抗原致敏DC。致敏DC与CIK共培养，制备经胃癌抗原致敏的DC诱导CIK细胞。

Extracellular particles, small mononuclear cells, red blood cells, and myoepithelial cells were markedly decreased in number.

胞外成分，小单核细胞，红细胞，肌上皮细胞数量上显著减少。

The optimal yield of MAPCs was obtained when mononuclear cells were cultured at a initial plating density of 1 × 10~6 cells / cm~2 and the medium was exchanged to remove the nonadherent cells after 72 hours of inoculation.

结果表明，在低血清条件下，DMEM／F12培养基更适合于脐带血MAPC生长；1×10~6／cm~2是原代培养的适宜接种密度，原代第72小时首次换液为最佳换液时问。

Methods: Nonadherent cells from bone marrow or peripheral blood mononuclear cells of 8 patients with chronic myeloid leukemia were cultured with rhGM- CSF and rhIL- 4. The cytokines were supplemented every three days. RhTNF-α was used at the ninth day. All the cells were harvested at the twelfth day.

从8例慢性髓性白血病患者的骨髓或外周血中获取非贴壁细胞，利用细胞因子(rhGM蛳CSF、rhIL蛳4、rhTNF蛳α)联合培养，每3 d补充新的细胞因子，其中rhTNF蛳α为第9天添加，第12天收获细胞。

Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient centrifugation. EGM-2 culture fluid was added, and then cells were plated on dishes coated with human fibronectin. After 48 h, the nonadherent cells were collected and replated on fibronectin-coated dishes.

采用Ficoll密度梯度离心法从人脐血中分离单个核细胞，加入EGM-2培养液，接种于包被人纤连蛋白的培养皿中贴壁培养，收集48h后的悬浮细胞重新贴壁培养至第7天，利用免疫荧光和流式细胞术鉴定。

METHODS: mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

METHODS: The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

On basis of mention , we investigate the ability of isolation, purification and expansion of adult rat mesenchymal stem cells to nerve cells in vitro.MethodsBone marrow mononuclear cells from adult rat femur and tibia were obtained sterilely by Percoll ( 1. 073 103 g/L ) gradient centrifugation .

根据MSCs与其它细胞的密度不同而采用Percoll(1.073×10~3g／L)分离液将其分离，此方法分离MSCs所获得的细胞种类较单一，Percoll分离液将骨髓细胞分为三层，取含MSCs的低密度层进行培养。

Result Mononuclear cells from C57B/6J mouse bone marrow was stimulated with rmGM-CSF and rmIL-4, with the typical growing pattern of DCs. At the third day, the cells became round or roundlike, semi-adherent clustering just like clusters of grapes. And little mixed cells with long apophyses can been seen.

结果 C57BL/6J小鼠骨髓单个核细胞在rmIL-4和rmGM-CSF刺激下，符合DCs生长的一般规律，即从第3天开始，细胞形态变为圆形或者类圆形，半贴壁，呈&葡萄串&或者呈簇生长；其中还可见到少量长突起的杂细胞。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。