查询词典 monocytes

A subpopulation of monocytes differentiates into DCs within 48 h in a model simulating transendothelial migration into lymphatic vessels.

一子单核细胞的区别纳入DCS在48小时内的模式模拟跨迁移到淋巴管。

The results showed that the peripheral blood consists of the following cell types:erythrocytes, Europhile, eosinophil, monocytes, lymphocyte and thrombocyte. The hemolysis time were 60s, 50s and 40s,respectively.

结果表明：在外周血细胞中可区分出红细胞，嗜中性粒细胞，嗜酸性粒细胞，单核细胞，淋巴细胞，血栓细胞等。

In the patient group, when comparing patients subject to active infection of any virus with those under no active infection, no difference of statistic significance between them is found in terms of age, course,frequency, admitted temperature, differential leukocyte count (neutrophilic granulocyte,achroacyte and monocytes),applying glucocorticoids or not, and EDSS point value.

病例组中任一病毒近期活动性感染者和无活动性感染者相比，其年龄、病程、发作次数、入院体温、血白细胞分类计数（中性粒细胞、淋巴细胞和单核细胞）、糖皮质激素应用与否及EDSS分值间的差异均无统计学意义。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

OBJECTIVE: Arteriogenesis, the growth of preexisting arterioles into functional arteries, is dependent on the proper function of monocytes.

目的：动脉的发生和生长均依赖于单核细胞的正常功能。

OBJECTIVE: Arteriogenesis, the growth of preexisting arterioles into functional arteries, is dependent on the proper function of monocytes.

摘要翻译：目的：动脉的发生和生长均依赖于单核细胞的正常功能。

The disease caused lesions primarily in the lungs, with histopathological changes manifesting interstitial hyperplasia, fibrin exudation, caseous necrosis, and lymphocytes, neutrophils and monocytes infiltration.

该病的病变主要集中于肺脏，其组织学变化主要是间质增生，纤维素渗出，干酪样坏死，淋巴细胞、嗜中性粒细胞和单核细胞浸润。

Methods Monocytes in peripheral blood were obtained from 12 patients with acute myocardial infarction, 16 unstable angina pectoris, 12 stable angina pectoris and 8 normals by ficoll density gradient centrifugalization. The cells were incubated for 48 hours to induce them to differentiate into macrophages by adding phorbolester 12-myristate 13-acetate. The levels of MMP-9 and TF in the supernatant were measured by ELISA.

密度梯度离心法提取12名急性心肌梗死、16名不稳定心绞痛、12名稳定型心绞痛患者和8名冠脉正常者的单核细胞，用佛波酯诱导其分化为巨噬细胞，ELISA法测定单核源巨噬细胞上清液MMP-9、TF含量。

Objective To investigate the variance of dendritic cells from monocytes of peripheral blood in patients with prime nephrotic syndrome.

目的：探讨树突状细胞的数量及功能在原发性肾病综合征时的变化及意义。

Blood samples were obtained on 0,2,4,6 and 8 days after admission. Dynamic changes of immunological parameters including serum IgA,IgG,IgM,complements (C3,C4) levels were determined in each group at various intervals following trauma. Phagocytosis of monocytes was also measured by use of phagotest kits after blood cells were incubated with fluorescin isothiocyanate-labeled E.

分别在入院时及入院后2、4、6和8 d留取外周静脉血，检测血清免疫球蛋白(IgA、IgG、IgM)与补体(C3、C4)水平的动态变化，全血细胞与用异硫氰酸荧光素标记的大肠杆菌共孵育后检测单核细胞噬菌能力。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。