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It was seen that strain 6055 generated three kinds of dextransucrases whose molecular weights were 151 kDa,142kDa and 117kDa,respectively.Among them,the former two ones were found both in supematants and cell wall,while the later was only found in the fermentation liquor.Strain DM 1-2 synthesized two kinds of dextransucrases,and their molecular weights were 183kDa and 142kDa,respectively.The former existed both in cell wall and out of cell,but the later only was located on cell surface.Strain PC 13 produced three kinds of dextransucrases,and their molecular weights were 148kDa, 138kDa and 115kDa,respectively.The former two ones were ectoenzyme while the later was linked to cell.Resembled to strain 6055,strain L4 produced two types of dextransucrases coexisting in supernatants and cell wall with molecular weights of 145 kDa and 136kDa,respectively,but the dextransucrase of 115kDa was only found in free-cells supernatants.

结果显示,菌株6055可产生分子量分别为151kDa、142kDa和117kDa的三种葡聚糖蔗糖酶,其中前两者同时存在于上清液中和细胞表面,而后者只存在于上清液当中;菌株DM1-2可以产生两种葡聚糖蔗糖酶,分子量分别为183kDa和142kDa,前者同时存在于上清液中和细胞表面,而后者仅能存在于细胞表面;菌株PC13可以产生分子量分别为148 kDa、138 kDa和115kDa的三种葡聚糖蔗糖酶,前两者属于胞外酶,而后者与细胞相连;与菌株6055相似,菌株L4可以产生两种同时存在于上清液和细胞表面的葡聚糖蔗糖酶,分子量分别为145 kDa和136kDa,而分子量为115kDa的葡聚糖蔗糖酶只存在于上清液中。

It is included that 4-5 types of active sites exist in the catalytic system and the active site produced polymer with low molecular weight is easy to be activiated while TEA is used as the cocatalyst. While TIBA, the active site produced polymer with high molecular weight and seldom active site produced polymer with low one is prone to be activiated. MAO tends to activiate the active site produced polymer with middle-level molecular weight. In TEA-TIBA catalytic system, TEA and TIBA activiate the corresponding active site and the weight of every active site gradationally varied with the change of the ratio of the two cocatalysts. And the activity of the active site produced polymer with high molecular weight is proportion to the concentration of TIBA.

发现:TEA作为助催化剂时产生低分子量产物的活性中心易于活化,且体系中具有活性的活性中心有4~5种;TIBA易于活化产生高分子量的活性中心,基本上不能活化产生低分子量产物的活性中心;MAO对产生中等分子量产物的活性中心活化能力较强;在TEA-TIBA混合物体系中,TEA和TIBA各自对活性中心的作用表现为明显的互补现象,各活性中心的相对强度随着两种助催化剂之间的比例改变呈现渐次变化,其中产生高分子量产物的活性中心的相对活性(来源:c5AB3fC论文网www.abclunwen.com)与TIBA的浓度有明显的正相关性。

Molecular biology molecular genetics and cell biology to promote the development of molecular immunology immunogenetics and immune biology the formation of new branches so that people at the molecular level on the structure and function of the immune system have a more understanding.

分子生物学、分子遗传学以及细胞生物学的发展促进了分子免疫学、免疫遗传学以及免疫生物学等新的分支学科的形成使人们在分子水平上对免疫系统的结构与功能有了更加深刻的熟悉。

The conclusion was drawn by the synthesis of molecular sieve: after mixed soluble glass and aluminium sulfate with proper proportion under room temperature, it was stirred stormily to react. And then we mixed with potassium hydroxide solution after filtrating. At last, the molecular sieves of potassium type was synthesized after the mixture reacted in the oven or autoclave for 24 hours. The molecular sieve was remodeled more than twice with ammonium chloride solution that the concentration was 3mol/L and then remodeled twice with the saturated sodium chloride solution. Finally the molecular sieve was changed into the sodium type. It was named W zeolite.

通过对分子筛的合成得到:以泡花碱、硫酸铝为原料,以硅铝比按一定比例于室温下将二者混合,激烈搅拌,抽滤后与氢氧化钾溶液混合放于烘箱或高压釜中高温晶化24小时,晶化后的分子筛为钾型,先后用3mol/L氯化铵溶液和饱和食盐水各对其改型两次以上,最后改为钠型,可制得W型沸石分子筛。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrin's (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明,R-PE的分子量为194kD,与目前报道的多数R-藻红蛋白240kD的分子量不同;其α、β亚基分子量均为20kD左右,γ亚基分子量约为31kD,理论推测的R-PE亚基组成为_4γ,与前人报道的_6γ不同,而且其是否存在第四亚基还有待进一步证明;同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrins (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明,R-PE的分子量为194kD,与目前报道的多数R-藻红蛋白240kD的分子量不同;其α、β亚基分子量均为20kD左右,γ亚基分子量约为31kD,理论推测的R-PE亚基组成为_4γ,与前人报道的_6γ不同,而且其是否存在第四亚基还有待进一步证明;同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001~0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点,远多于S群体(14)和G(21)群体,而且在53个特有位点中有9个出现频率为100%的位点,这些位点可以作为区分其它2个群体的特征性标记;S– G群体特有的位点有112个,其中有4个位点出现频率为100%,可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585,遗传距离只有0.0424,在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起,说明二者的亲缘关系很近,应属于种内群体间的关系;而W与S和G的遗传相似性系数均较小(0.7939和0.7941),相对遗传距离很大而且十分相近(0.2308和0.2305),在亲缘关系树状图上单独分出一支,也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现,W的ITS序列长度在1266-1269 bp,而S和与G的ITS序列总长度分别为1520 bp和1614 bp;从ITS1和ITS2长度来看,W分别为739-741 bp和316-317 bp,S为895 bp和414 bp,G为987 bp和416 bp;而从ITS碱基组成来看,W的GC含量在62.32-62.62%之间,而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003,S与G群体间的遗传距离是0.010,说明W群体内变异很小,而S与G群体间已出现明显的遗传分化,但还均属于种内群体间的遗传变异;而W与G和S的遗传距离分别达到0.110、0.147,两个类群差异显著,已远超出种内群体间的遗传变异。

Keywords molecular simulation;heavy components;polymethylene bridges;molecular conformation;molecular mechanism;molecular dynamics

分子模拟;重组分;桥链;分子结构;分子力学;分子动力学

Domestic and overseas entomologist has showed that different molecular marker methods for molecular identification of Trichogramma species. However, each of molecular marker methods previously has own deficiency, and molecular marker genes used in Trichogramma species identification almost is ribosomal DNA, study about mitochomadrial DNA in Trichogramma spp. is infrequent.

近年来,随着分子生物技术的发展,国内外研究者曾用不同分子标记方法来进行赤眼蜂蜂种的分子鉴别,然而每种分子标记都有自己的不足,且赤眼蜂分子鉴定中用于序列分析的标记基因基本上为核糖体核糖核酸基因,而有关线粒体DNA的研究却鲜有报道。

RESULTS Degra-dation of PCL was the slowest one.Mass-loss was not obvi-ous:molecular mass of pancreatic suspension lost by19.8%and no outstanding physical change was observed.PCL2/1loses mass by0.5%in24wk,and molecular mass loses by61.7%with etches on cuttings sections.PCLA1/2loses mass by40.4in24wk,molecular mass loses by6.6%,and cracks and holes were observed as the main physical charac-ter.PLA lost mass by79.2%in24wk,molecular mass lost by15.1%and this material was totally degraded.

结果 PCL在24wk内各种性质变化最慢:质量损失不明显,分子质量在胰腺悬液中下降19.8%,形态无明显变化;PCLA2/1在24wk时质量损失0.5%,分子质量下降61.7%,材料断面出现蚀刻形态;PCLA1/2在24wk质量损失40.4%,分子质量下降6.6%,形态出现空洞、碎裂;PLA在24wk质量损失79.2%,分子质量下降15.1%,形态完全降解。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

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