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Proliferations of cells were observed by MTT colorimetric assay and determination of total protein level in cells.

用体外制备的外源性糖基化终末产物和平滑肌细胞共孵育24h，加入不同浓度的cariporide（0.1、1.0和10μmol/L）处理。

A hydroponical experiment was carried out to study the toxicity of copper, cadmium and their combined pollution on ultrastructure of Commelina bengalensis Linn. Transmission Electron Microscope was used to investigate the effect of heavy metal on ultrastructure of different parts of plants.

为了解铜、镉对泡泡草根、茎、叶细胞亚显微结构的毒害及作用位点，以期为进一步研究鸭跖草属植物的耐重金属机理提供理论基础，本文通过透射电子显微技术研究了75 μmol·L-1铜和50μml·L-1镉及其交互污染对泡泡草根、茎、叶细胞超微结构的影响和铜、镉在细胞中的分布。

In this investigation,the composition of copolymer P(AM- co -NaAMPS) of acrylamide and 2-acrylamido-2-methyl-propanesulfonate sodium has been determined with the aqueous solution of cetyl trimethyl ammonium bromide as titrant at a concentration of 0.001 mol·L-1 by conductometric titration method.

与元素分析进行比较的结果表明，上述复合作用电导滴定法可作为测定水溶性大分子链中磺酸盐单体含量的方便而又准确的方法，而且预计还可用作为测定水溶性大分子链中其它离子性基团含量的简捷方法。

The factors affect on the degree of substitution of product, yield and apparent viscosity, such as the mol rates of EPTAC with -NH2, reaction temperature, reaction time and the content of water in system have been discussed in detail. FTIR spectrometer and UV spectrometer are used to characterize the structure of the derivative and it is proved that substitution reaction mainly occurs on the -N element .The particle size and morphology of chitosan and quantemary ammonium salt of chitosan are analysised by comparing photos of their scanning electron microscope. The degree of substitution of HACC is determined by acid -base conductometric titration. An error in current formula of DS has pointed out and a correct formula has presented.On the base of experience of above experiment, the reaction conditions of HACC were systematically investigated through orthogonal design, and then the best conditions are found. Water-solubility and dependence of pH are studied.

壳聚糖和环氧丙基三甲基氯化铵在异相条件下发生反应，通过单因素实验，制备了一系列不同性能的壳聚糖季铵盐，本文首次详细讨论了反应条件(反应摩尔比、反应时间、反应温度、水含量)对产率及产物性能的影响；用红外光谱和核磁共振对结构进行表征分析时，发现季铵盐阳离子基以N位上取代为主；通过电镜图片对壳聚糖和季铵盐衍生物外观形貌进行对比，采用酸碱电导滴定法测定产物的取代度，并对常用取代度公式做了修正。

Mol/L and calcinations temperature at 500?℃. The solid superacid can be used as catalyst two times in the alkylation of naphthalene, and the total composition of MIPN,DIPN, and TIPN in product is over 98%. When the consumpt...

在催化剂用量为 10 %、反应温度为 160℃、反应时间为 2 5h的条件下连续两次使用SO2 - 4 TiO2 超酸催化萘对丙烯的烷基化反应，产品中一异丙基萘、二异丙基萘、三异丙基萘的总含量大于 98%。

Grafting degree increased with increasing monomer concentration in acrylic acid and crotonic acid systems. In undecylenic acid and oleic acid systems, grafting degree increased with monomer concentration initially but decreased after monomer concentration reaching at 0.30 mol/L.

丙烯酸、反式丁烯酸体系的接枝率随单体浓度的增加而增大。10-十一烯酸、油酸体系的接枝率初始时随单体浓度的增加而增大，单体浓度超过0.30mol／L后，接枝率降低。

Results: The effects of occlusion of dentinal tubules occurred after treatment with 0.6 mol/L solution,the most tubules could be obs...

1.2mol/L浓度的溶液组效果与前一组无明显差异。结果：1mol/L浓度的碳酸氢钠溶液与牙体组织发生反应的生成物碳酸磷灰石能有效地堵塞牙本质小管

After collecting the bacterial cells and breaking the cell by means of ultrasonic, the inclusion bodieswere extracted. The extracted products were denatured with 8 mol/L urea and purified through Ni-NTAcolumn. The purified products were renatured by dialyzation, and then were analyzed by SDS-PAGE.

在优化条件下诱导重组菌表达，收集菌体经超声波破碎和裂解后提取不溶性包涵体，以8mol／L尿素处理包涵体提取物并进行Ni-NTA亲和层析纯化，纯化表达产物经透析复性后以SDS-PAGE分析其复性效果。

The powder of cement was composed of calcium carbonate, tricalcium phosphate and calcium phosphate dibasic.

再以磷酸氢二钠和磷酸二氢钠合成浓度为0.2 mol/L的磷酸钠缓冲液，作为固化液。

Factors of esterfication of mixed dibasic acid were studied: alcohol acid mol ratios, the content of the catalysts, the reaction time and the content of water carrier.

实验考察了混合二元酸酯化的影响因素：醇酸摩尔比、催化剂用量、反应时间以及带水剂的种类和用量对酯化率的影响。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。