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On the normotensive rat, bilateral microinjection of L-arginine (16 n mol/0.1 ul/site), a precursor of nitric oxide into the rVLM, caused a decrease in BP, HR and the pressor response induced by the dPAG stimulation, while microinjection of NG-methyl-Larginine (L-NMMA, 16 n mol/0.1 ul/site), a blockade for NO to form, into the rVLM bilaterally, caused an increase in BP, HR and the pressor response induced by the dPAG stimulation. There was no effect of both drugs mentioned above on the tachycardiac response induced by the dPAG stimulation.

五、在正常血压大鼠,于双侧rVLM微量注射一氧化氮前体(16nmol/0.1μl/site)可引起BP降低、HR减慢、以及电刺激dPAG诱发的防御性升压反应减弱;而于同区微量注射生成一氧化氮的拮抗剂N〓-甲基-L-精氨酸(16nmol/0.1μl/site)则可引起BP升高、HR加快、以及电刺激dPAG诱发的防御性升压反应增强;而上述两药对电刺激dPAG诱发的防御性加快心率反应均无明显作用。

The effects of solution\'s pH value, mol ratio between phenyl aldehyde and L-Arginine, concentrations of sodium chloride, ammonia chloride andL-Lystine, initial concentration of L-Arginine on precipitation rate of a -tolylene arginine were investigated, the results indicated that phenyl aldehyde precipitation method\'s appropriate pH value was higher than 11, suitable mol ratio between phenyl aldehyde and L-Arginine was 1.25, initial concentration of L-Arginine was must higher than 15g/L, sodium chloride had little effect on precipitation rate, on the contrary, ammonia chloride and L-Lystine had great effect on it, both of them must be eliminated; At the same time, adsorption isotherm at 25 C of L-Lystine on anion exchange resin A was measured, the result showed that the maximal equilibrium adsorbance was 30mg/g, the influences of solution\'s pH value, temperature, concentration of chloride ion on adsorption of L-Lystine by anion exchange resin A were also studied, the results indicated that appropriate operational pH value was 0.5 0.2, the effect of temperature on adsorption ratio was little, process could be operated at room temperature, the concentration of chloride ion must be eliminated.

考察了溶液pH值、苯甲醛与L-精氨酸摩尔比、氯化钠与氯化铵浓度、L-赖氨酸浓度、L-精氨酸初始浓度对苯甲醛沉淀L-精氨酸的影响,结果表明,沉淀反应的适宜pH值大于11,苯甲醛与L-精氨酸的适宜摩尔比为1.25,(来源:Aa6BC论文网www.abclunwen.com)L-精氨酸适宜的初始浓度在15g/L以上,氯化钠的存在对沉淀率基本上没有影响,而氯化铵和L-赖氨酸的存在使沉淀率下降;同时测定了25℃时阴离子交换树脂A吸附L-赖氨酸的吸附等温线,表明其最大平衡吸附量为30mg/g,考察了溶液pH值、温度、氯离子浓度对阴离子交换树脂A吸附脱除L-赖氨酸的影响,结果显示从L-精氨酸和L-赖氨酸混合液中吸附分离出L-赖氨酸的适宜pH值为10.5±0.2,温度对L-赖氨酸吸附率影响不大,吸附过程可在室温下进行,氯离子的存在使L-赖氨酸的吸附率降低。

In this study, the critical point data of ternary systems which is composed of supercritical carbon dioxide, trisodium salt of tri-(m-sulfonphenyl)phosphine and cosolvent were measured the first time. With the increase of mol% of the cosolvent, critical temperature of ternary system increased, and the experimental data show a linear dependence of critical temperature with mol% of the ternary system over the concentration ranges studied too.

北京化工大学硕士研究生论文本文还首次测试了在超临界CO:中加入三磺化三苯基麟以及共溶剂组成的三元体系的临界点数据,发现三元体系中临界温度也随共溶剂的浓度增加逐渐增加,呈一种线性关系。

No considerable effect was observed on the COX activity of P.falciparum treated with daphnetin-Fe complex. The tyrosyl free radical was tested to reflect the RNR activity of P.falciparum at various times by ESR.

结果 体外同步培养的恶性疟原虫经瑞香素(100 μmol/L)作用2、4、8和12 h后,COX活性分别被抑制了0、6%、73%和80%;在瑞香素浓度为0.1、1、100和1 000 μmol/L,作用6 h后,COX活性分别被抑制3%、31%、53%和84%;而瑞香素-Fe复合物对COX的影响几乎消失。

And probable mechanism for the solubility differences was illuminated by the analysis of unreduced SDS-PAGE. Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响;rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍;添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

Results There were favourable dependability between vanadic acid oxidation method, improved J - G method and Bilirubin Oxidase method.

结果:钒酸氧化法与改良J—G法、胆红素氧化酶法有良好的相关性;其回收率均在95.0%~105.0%;其线形范围分别是:0~678μmol/L,0~634μmol/L,0~575μmolAL。

Thus. Treatment of CR1R2=C=CH2 (R1 = R2 = Me; R1 = H, R2 = n-Bu; R2 = Ph; R2 = cyclohexyl; R2 = cyclopentyl, R2 = C6H5O, R2 = 2-IC6H4O, R2 = 4-IC6H4O) with bisdiborn in the presence of Pd2 (5 mol %) and 3-iodo-2-methyl-2-cyclohexen-1-one (5 mol %) in toluene at 80 oC gave products bearing both vinylic and allylic boron moieties in good to excellent yields.

但我们发现可以使用Pd金属错合物与有机碘化物共同催化下的系统,来进行此种加成反应。溶剂的选择以甲苯为最佳,Pd错合物以没有强配位基存在为最好的选择。

The morbidity of RCN was 14%(7/50) in sodium chlorid group while 1.82%(1/55) in sodium bicarbonate group.

血清肌酐水平在造影前碳酸氢钠组高于生理盐水组[(110.5±27.4)μmol/Lvs(102.5±13.3)μmol/L, P=0.043]。

Methods The spontaneous contraction of gastric smooth muscle strip was recorded by using physiograph in rats. The change of tension in response to different concentrations of CNP and sodium nitroprusside, co-incubated with high concentration glucose (30 mmol/L) or mannitol were detected.

取大鼠40只制备胃窦环形肌条,肌条收缩活动稳定后,对照1、2组分别加入不同浓度CNP1×10^(-8、3×10^(-8)、1×10^(-7)mol/L及硝普钠1×10^(-7、3×10^(-7)、1×10^(-6)mol/L,观察其效应,每次加药观察20 min,冲洗3次;高糖1、2组预加葡萄糖使浴槽终浓度为30 mmol/L,其后操作同对照1、2组;甘露醇组预加甘露醇30mmol/L,其后操作同对照1组;均采用多道生理信号采集处理系统计算CNP致平滑肌张力的抑制率。

The best reaction system for ISSR in our research followed as: the whole volume of the reaction is 20μL, and the mixture in this reaction included 50ng DNA, 2.0mmol/L MgCl_2, 0.2μmol/L primer, 200μmol/LdNTPs, 0.5U TaqDNA polymerise and 1×PCR buffer; and the PCR program was 94℃7min, 1 cycle; 94 ℃ 30s, specific temperature 45s, 72℃ 90s, 38 cycles; 72℃ 10min, 1cycle.

对两种标记的检测效果表明,ISSR和RAPD标记所得结果呈极显著相关,证明这两种标记技术均可用于樟科植物的遗传多样性和亲缘关系研究,但ISSR效果明显优于RAPD。

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