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The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

The results showed that the photosynthesis of Melinis minutiflora varied in a double-peak pattern with a remarkable noon inhibition and being controlled by stoma. The first peak appeared around 10:00 am while the Pn reached 18.8 molCO2/(m^2s) and the second peak appeared around 14:00 pm while the Pn reached 14.4 molCO2/(m^2s), and changed positively with the variation of transpiration rate and stomatal conductance.

结果表明，糖蜜草的光合日变化呈双峰型，有明显的&午休&现象且受气孔调节。10：00出现第1个峰值，Pn为18.8μmol/，14：00左右出现第2个峰值，Pn为14.4μmol/，变化趋势与蒸腾速率、气孔导度等因子相同。

The transition states of the reaction between THF、 butylene oxide and secondary oxonium ion were acquired. By using frequency analysis and inner reactioncoordinate analysis the rationality of these transition states were affirmed.

分别对四氢呋喃和环氧丁烷，绘出了链增长反应的势能面曲线，得到四氢呋喃链增长反应的活化能为116.749kJ/mol；环氧丁烷链增长反应的活化能为92.672kJ/mol。

Methods Periosteal cells were isolated and cultured in vitro and then exposed to dexamethasone 10 -8 mol/L, 10 -7 mol/L an...

利用多种细胞因子促进细胞向成骨细胞的分化，以保证细胞在植入体内后能够保持并充分发挥其成骨活

The reaction rate constants of Pb〓 with pheophytin a, pheophytin b and hydroxypheophytin a were 2. 0 mol〓 l s〓, 0.78mol〓 l s〓and 1. 86 mol〓 l s〓, respectively.

铅与叶绿素a直接进行反应的结果显示：铅并不能取代叶绿素中的镁生成铅叶绿素。

Photooxidative characteristics and physiological mechanism in leaves of super high yielding hybrid rice Peiai 64S/E32 and its parental lines were investigated by photooxidative treatment mediated with 200 μmol/L of exogenous methyl viologen under a PPFD of 2 000 μmol·m -2 ·s -1 for 1 h.

结果表明相对于含有粳稻成分的母本和高产子代而言，籼稻父本是光氧化敏感型，而子代超高产水稻耐光氧化的特性主要受含粳稻成分的籼稻母本的遗传控制。

For example, the ratio of pyrogenation will reach 90% at around 600℃, and we can get 1.8 mol hydrogen from one mol methanol.

温度在600℃左右时，甲醇热分解率达90%，每摩尔的甲醇可产生1.8摩尔左右的氢气。

A higher yield(about 80%) can be obtained when 0.20-0.25 mol/1 of TUPMA is refluxed in 0.5 mol/l of NaOH solution at 75℃for 8h.

该产物的熔点为165.3℃，MS、~1HNMR和IR综合分析的结果确认，该产物的结构是4-二(1，2，4-三唑-5-硫酮基)-1，5-苯二甲酸。

By means of differential centrifugation,the studies on copper distributing character in subcell of Setcreasea purpurea Boom.showed that Cu content mainly distributed in cell wall and Cu content in chloroplast was a majority of whole leaf Cu content.

在不同浓度Cu供应时，紫鸭跖草对其必需养分元素的吸收和积累维持正常，它们的浓度均在保证一般植物正常生长的浓度范围内。100μmol·L~(-1)～250μmol·L~(-1) Cu的供应能明显促进植物生长。

Butyl p-hydroxybenzoate was synthesized from p-hydroxybenzoic acid and butanol with sulfoacid resin as catalyst. The optimum conditions were as follow: ratio of n-butanol, p-hydroxybenzoic acid and sulfonic acid resin was 0.25 mol ∶ 0.05 mol ∶ 1.5 g, refluxing for 5 h using water separator at the reflow reaction temperature.

摘 要：以对羟基苯甲酸和丁醇为原料，用磺酸树脂作催化剂，催化合成了尼泊金丁酯，优化反应条件为：n∶ n=5 ∶ 1，催化剂磺酸树脂的用量为对羟基苯甲酸质量的21%，反应温度为回流温度，反应时间为5 h。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。