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The pH value of UP solution at different concentration were determined by PHS-2C numerable acidimeter.

用数学方法预测出磷酸脲在不同溶剂[w(H_3PO_4)=0，0.04390，0.1091，0.2160]中的熔解焓分别为-20.54kJ·mol~(-1)，-27.50kJ·mol~(-1)，-35.31kJ·mol~(-1)和-32.54kJ·mol~(-1)。

In order to research the relations of carbon sources to structure of PHAs, six kinds of PHAs sample were synthesized by activated sludge with six different carbon sources respectively in the aerobic-anaerobic-anoxic process. Their structures were determined by 〓HNMR、〓CNMR and GC-MS. When the carbon source was acetate, the unit composition of PHAs was 93. 91mol％ 3-hydroxybutanoic acid and 6. 09mol％ 3-hydroxypentanoic acid ; When the carbon source was propionate, the unit composition of PHAs was 28. 66mol％ HB, 63. 13mol％HV, 2. 55mol％ 2-methy-3-hydroxy-butanoic acid (2MHB) and 5. 66mol％ 2-methyl-3-hydroxypentanoic acid (2MHV); When the carbon source was 80％ butyrate and 20％ 1, 4-butanediol, the unit composition of PHAs was 65. 03mol％ HB, 28. 06mol％HV, 1. 91mol％2MHB, 2. 69mol％ 2MHV, 0.73mol％ 4-hydroxy butanoic acid (4HB), 0.78mol％ 4-methyl-3-hydroxy-pentanoic acid (4MHV), 0.80mol％ 3-hydroxyhexanoic acid ; When the carbon source was 20％ butyrate and 80％ 1, 4-butanediol, the unit composition of PHAs was 61. 39mol％ HB, 23. 01mol％HV, 4. 58mol％2MHB, 5. 97mol％ 2MHV, 0.91mol％ 4HB, 2. 37mol％ 4MHV, 1. 77mol％ HHx; When the carbon source was 60％ pentanoate and 40％glucose, the unit composition of PHAs was 41. 24mol％ HB, 58. 76mol％HV; When the carbon source was 60％ benzoate and 40％ glucose, the unit composition of PHAs was 65. 48mol％ HB and 34. 52mol％ HV.

为了研究不同碳源与产物PHAs结构的关系，采用好氧-厌氧-缺氧模式利用6种不同碳源培养活性污泥得到6种不同的PHAs样品，通过〓HNMR、〓CNMR和GC-MS谱图确定了这6种PHAs的单体成分和比例：以乙酸钠培养活性污泥得到的PHAs的单体组成是93.91mol％3-羟基丁酸和6.09mol％3-羟基戊酸；以丙酸钠培养活性污泥得到的PHAs的单体组成除28.66mol％HB、63.13mol％HV外，另外还有2.55mol％3-羟基2-甲基丁酸（2MHB）和5.66mol％3-羟基2-甲基戊酸（2MHV）；以80％丁酸钠和20％1，4-丁二醇混合溶液培养活性污泥得到的PHAs含有七种单体组成：65.03mol％HB，28.06mol％HV，1.91mol％2MHB，2.69mol％2MHV，0.73mol％4-HB（4-羟基丁酸），0.78mol％4MHV（3-羟基-4-甲基戊酸），0.80％HHx（3-羟基己酸）；以20％丁酸钠和80％1，4-丁二醇培养活性污泥所得到的PHAs含有与3#样品相同的七种单体，只是比例不同，分别是61.39mol％，23.01mol％，4.58mol％，5.97mol％，0.91mol％，2.37mol％，1.77mol％；以60％戊酸钠和40％葡萄糖培养活性污泥所得到的PHAs由HB和HV组成，比例是41.24 mol％∶58.76 mol％；以60％苯甲酸钠和40％葡萄糖培养活性污泥所得到的PHAs也由HB和HV组成，比例是65.48 mol％∶34.52 mol％。

Under the above conditions,the extraction yield of total flavonoids was 20.6μmol/g,rutin was 11.2μmol/g,quercitrin 5.6μmol/g,epicatechin 3.8μmol/g.

结果表明，苦荞麸皮黄酮类化合物的最佳提取工艺条件为微波加热时间15min，乙醇体积分数40%，压力400kPa。

Mono-cinnamyl maleate was prepared by esterification of maleic anhydride and cinnamyl alcohol of 1 mol:2 mol at 95 ℃ for 2～4 h.Then added isomeric catalyst and mono-cinnamyl fumarate was made by reacting at 90 ℃ for 4 h.After methanol (methanol:mono-cinnamyl fumarate = 6 mol:1 mol) was added,the mixture maintained at 90 ℃ for 4.0 h to synthesize CMF.

合成试验表明：以摩尔比为1：2的马来酸酐与肉桂醇在95 ℃醇解反应2～4 h后加入异构化催化剂，90 ℃转型反应4 h后，添加6倍摩尔质量的甲醇，在90 ℃恒温反应4 h，反丁烯二酸桂醇甲酯的合成产率较高。

The precision, linearity, anti -interference ability, recovery rate were compared among the 4 methods according to the standard method of NCCLS, and relativity between each of the 4 methods and modified diazotate J - G was calculated. Results

结果改良化学氧化法、钒酸氧化法、胆红素氧化酶法的批内精密度均小于3.0％；4种方法的回收率均在95.0％～105.0％之间；KHC3法、改良化学氧化法、钒酸氧化法和胆红素氧化酶法的线性范围分别是：0～424μmol／L、0-675μmol／L、0-629μmol／L、0-575μmol／L；胆红素氧化酶法和改良化学氧化法均有较强的抗干扰能力，和改良J-G法的测定结果有高度一致性。

The outward rectifier K+ current current was the dominant K+ current in the whole cell currents in Deiters' cells. In the presence of 200μ M linopirdine, the Ik current was not significantly effected. Our findings demonstrated that the KCNQ heteromeric or homomeric potassium channel may be the molecular basis for the peak outward K+ current and testified that the inward IKn current is mediated by KCNQ potassium channel.

在细胞外液中，加入100 μmol/L利诺吡啶后，OHCs中的四乙基二乙胺敏感的钾电流峰电流成分被抑制，稳态电流幅值减小，且电流的失活时间常数明显延长；在细胞外液中加入100 μmol/L和200 μmol/L利诺吡啶后，OHCs的内向性钾电流IKn被完全抑制；而细胞外液中利诺吡啶终浓度为200 μmol/L 时，Deiters细胞的外向整流性钾电流幅值无明显变化。

Reverse-transcription PCR assays were used to detect the mRNA expression level of related regulatory genes such as p15, p16, p21, p27, p57, surviving, cyclin B, cdc2 and chk1, and Western blot assay to detect the level of protein expression and phosphated change such as survivin, cdc2 and chk1. Results:(1) K562 cell arrested on G2/M phase were obviously increased after co-cultured with 2 to 10μmol/L Arsenic trioxide for 24 hours. The ratios of control group, 2μmol/L, 5μmol/L and 10μmol/L groups were 22.6±3.4%, 27.2±2.3%, 43.8±4.5% and 36.7±4.1%, respectively.

体外培养K562细胞，以不同浓度AS_2O_3作用不同时间后采用PI染色、流式细胞仪检测药物作用后细胞的周期分布改变；逆转录酶多聚酶链扩增方法检测细胞周期相关调节基因(p15、p16、p21、p27、p57、survivin、cyclinB、cdc2、chk1)的mRNA表达变化；Western blot方法检测细胞周期相关调节蛋白表达及磷酸化改变(survivin、cdc2、cdc2-p、chk1)。

The results showed that the light saturation point of potato leaf was about 1400 μmoll(m^2s) and its light compensation point was about 50 μmoll(m^2s), the stomatic conductance and the transpiration rate increased while the intercellular CO2 concentration reduced with the increasing of light intensity between the light compensation point and light saturation point.

试验结果表明：马铃薯叶片光合作用的光饱和点约为1400 μmol/(m^2s)，光补偿点约为50μmol/(m^2s)。在光饱和点和光补偿点之间，随著光合强度的增加，叶片的气孔导度和蒸腾速率升高，胞间CO2浓度降低；随著CO2浓度的增加，光合速率升高，其CO2的饱和点约为2000μmol/mol。

The UV/vis exhibited the absorbance of BSA(210nm) to shift tored and decrease gradually with the addition of nicotine, reflecting the transition of secondarystructure of BSA, namely the helix of BSA become looser.

Lineweaver-Burk 双倒数方程计算pH7.4 和 pH11.0 体系在温度为 20℃和 37℃条件下尼古丁和 BSA 的结合常数 k 分别为： k20℃=140.15 L·mol-1 ，k37℃=131.83 L·mol-1 （pH7.4）和 k20℃=141.76 L·mol-1，k20℃=27.79L·mol-1(pH11.0)，表明结合常数在 pH7.4 条件下受温度的影响要比 pH11.0 条件下小，推测是由于不同 pH 下尼古丁存在的不同形态所致。

Most sugars, organic acids, phenolic acids and most amino acids increased significantly with elevated CO_2. The treatment, 950±50μmol·mol-1 CO_2 enrichment for 6 h every day had more obvious effects than the treatment, 950±50μmol·mol-1 CO_2 enrichment for 3 h every day. Levan, sucrose, arginine, oxalic acid, alanine, malic acid, acetic acid, lactic acid cinnamic acid,ρ- hydroxybenzoic acid and benzoic acid increased significantly.

CO_2施肥黄瓜根系分泌有机酸、氨基酸、糖和酚酸的总量增加，上、下午均施肥的处理分泌数量最多；CO_2施肥显著促进了果聚糖、蔗糖、草酸、苹果酸、乙酸、乳酸、多数氨基酸、肉桂酸、对羟基苯甲酸和苯甲酸等组分的分泌；单位鲜重根系分泌糖和酚酸的数量增加，多数氨基酸保持不变或降低，但有机酸分泌规律不一致。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。