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The first part consists of three experiments:(1) The rings were incubated in KH, 20, 50 mmol/L 〓 for 1 house, relaxation in response to the EDHF stimuli A23187 in 30nmol/L U46619-induced preconrtaction in the presence of 7 μ mol/L indomethacin, a cyclooxygenase inhibitor, 300μmol/L LNNA, a nitric oxide biosyhnthesis inhibitor, and 1mmol/L tetraethylammonium , a 〓 blocker, or 3 μmol/L glibenclamide , a 〓 blocker, was compared with the control;(2) After the arteries were incubated in KH, UW solution or HTK solution at 4℃ for 4 hours, endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μmol/L indomethacir and 300μmol/L LNNA;(3) After incubation with KH, UW solution and STH (either at 37℃ in oxygenated organ chamber or at 4℃ in a refrigerator for 4 hours), endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μ mol/L indomethacin and 300 μmol/L LNNA.
第一部分研究结果:(1)单纯浸泡于KH的冠状动脉A23187能引发66.67%的血管舒张反应,经TEA及20mmol/L、50mmol/L钾离子作用后,血管舒张反应程度显著降低,但经GBM作用后改变不明显;(2)与保存于KH的冠状动脉相比,A23187引发的血管舒张反应程度,保存于UW液的明显下降,保存于HTK液的无明显变化;(3)在37℃条件下,血管环浸泡于STH出现缓慢轻微的舒张反应,浸泡于UW液初期出现短暂收缩反应,但此后主要以舒张为主:在37℃条件下,血管经UW液保存后,U46619引发的收缩反应程度降低;不论在37℃或4℃条件下,A23187引发的血管舒张反应,经UW液保存后明显下降,但用STH保存后变化不明显。
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The isopiestic molalities and water activities have been determined for the systems Li_2SO_4-H_2O and Li_2B_4O_7-H_2O at 273.15K in the ion strength ranges of 0.6063~ 1.7640 mol · kg~(-1),and 0.1069—0.6478 mol· kg~(-1) respectively and for the systems Li_2SO_4-H_2O, Li_2B_4O_7-H_2O and Li_2SO_4-Li_2B_4O_7-H_2O at 298.15K in the ion strength ranges of 0.3577—5.6378 mol·kg~(-1), 0.1747—2.4497 mol·kg~(-1), 0.2394—5.5399 mol · kg~(-1) respectively by the isopiestic method using an improved equipment.
本论文利用改进的等压设备和实验方法测定了273.15K下纯盐水溶液Li_2SO_4和Li_2B_4O_7离子强度范围分别为0.6063~1.7640mol·kg~(-1,0.1069~0.6478mol·kg~(-1)以及298.15K下纯盐水溶液Li_2SO_4和Li_2B_4O_7离子强度范围分别为0.3577~5.6378mol·kg~(-1,0.1747~2.4497mol·kg~(-1)和Li_2SO_4-Li_2B_4O_7-H_2O体系混合盐水溶液离子强度范围为0.2394~5.5399mol·kg~(-1的等压平衡浓度和水活度。
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In the presence of yttrium, lutetium and gadolinium, the fluorescence intensity of the system Eu-SPLX-SDS was enhanced about 5-fold, 5-fold and 4-fold compared with that of the system without yttrium, lutetium and gadolinium.
在确定的最佳实验条件下,铕-SPLX-phen-SDS,铕-SPLX-SDS配位体增敏荧光体系和铕-钇-SPLX-SDS稀土元素共发光体系的荧光强度同铕的浓度分别在5.0×10〓~1.0×10〓mol/L,5.0×10〓~1.0×10〓mol/L,5.0×10〓~5.0×10〓mol/L,1.0×10〓~5.0×10〓mol/L和5.0×10〓~1.0×10〓mol/L范围内呈良好的线性关系,稀土元素铕的检测限分别为1.0×10〓mol/L,7.0×10〓mol/L,1.0×10〓mol/L,2.0×10〓mol/L和4.0×10〓mol/L。
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As to the numbers of culturable heterotrophic bacteria, there was different relationship between them and the environmental parameters in different seasons, and significant positive relationship between them and glucosidase activities.
结果表明,冬季与夏季各环境参数之间有显著差异;各站位α-葡萄糖苷酶活性的变化范围分别为10.63μmol/gh-100.86μmol/gh和43.80μmol/gh-197.78μmol/gh;β-葡萄糖苷酶活性的变化范围分别为39.60μmol/gh-222.75μmol/gh和169.88μmol/gh-676.9。。。
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The effects of the chelator and inhibitors of PKC and PI3-K on fMLP-induced the activation of Akt were also examined.
结果10μmol/L BAPTA-AM和8μmol/L GF109293x均明显地抑制过氧化物产生;50μmol/L SB203580和50μmol/LPD098059分别抑制50%和60%过氧化物生成;0.1μmol/Lwortmannin仅抑制30%过氧化物生成。
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High-resolution 〓H-NMR spectra were used to confirm the formation of the ICs, and XPS spectra were used to corroborate the formation of S-Au bonds. The significantly improved"reversible"voltammetric responses and the reproducible surface coverages of (2.4±0.2)×10〓 and (2.2±0.2)×10〓 mol/cm〓 obtained for SAMs of β-CD/C〓VC〓SH and α-CD/C〓VC〓SH, respectively, indicate that the intercalated CD can effectively dilute the surface viologen groups and weaken the intermolecular interaction, giving a wellordered and viologen-containing SAM with a determinate surface coverage.
高分辨〓H-NMR谱确证了预期包络物的形成;XPS谱确证了包络物电活性自组膜和S-Au共价键的形成;明显改善的&可逆&伏安响应和重现的紫精基团表面覆盖度[对β和α分别为(2.4±0.1)×10〓和(2.2±0.1)×10〓mol/cm〓],表明嵌入的环糊精可有效地&稀释&紫精基团,在很大程度上减弱它们之间的相互作用,形成了完备的和有确定紫精覆盖度的包络物电活性自组膜。
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Once the temperature of the environment exceeds the critical explosion temperature, the delayed-action time will shorten along with the temperature of the environment ascending;(2) The thermal decomposition reactivity activation energy and correlative parameters of the emulsion explosive matrix and corresponding mixture of emulison explosive with combustible oil solvent were obtained by iron-plate experiment, thereinto, the activation energy of the emulsion explosive matrix is 163.3kJ/mol.
一旦环境温度超过临界爆炸温度,随环境温度的升高,爆炸延滞期将变短;(2)通过铁板实验获得了乳化炸药基质及其与有机溶剂混合物的活化能和相关参数,其中乳化炸药基质的活化能为163.3kJ/mol。
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Bcl-2 mRNA was detected by RT-PCR in all cells;(2)After normal rat and bile duct-ligated 14d rat hepatocytes were added 100 μmol/L GCDC and kept for 24 hours, cells were evaluated by FCM and TUNEL. Results:(1)Normal rat hepatocytes did not express Bcl-2 by RT-PCR technique. Bcl-2 was expressed in 7-,14-,21- day BDL rats.
分离对照组及实验组14 d组大鼠的肝细胞行原代培养后,使用100 μmol/L甘氨鹅脱氧胆酸钠作用于两组肝细胞24 h后,用FCM法测定肝细胞的凋亡比率,用TUNEL技术进行肝细胞凋亡的原位检测。
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The reaction kinetics was studied by selecting control-step method as well as modified Debye-Hückel activity coefficient model and Bromley osmotic coefficient model. The results show that the reaction order is 2 and apparent reaction activation energy 85.8 kJ/mol, and the reaction is mainly controlled by precipitation of KB5O8?4H2O.
采用选取控制步骤法并引入修正的Debye-Hückel活度系数模型和Bromley渗透系数模型研究了该反应的动力学的影响规律,得出反应级数为2,表观反应活化能为85.8 kJ/mol,反应主要受四水五硼酸钾晶体析出的控制。
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Electrode potential was in direct proportion to concentration of NO〓〓 in range of 1.0×10〓 and 50×10〓mol/L slope of Nernst response is
并用选择性电极实时观察了亚硝酸根在聚吡咯膜中的掺杂=释放过程,在1.0×10〓~5.0×10〓mol/L浓度范围内,电极电位与亚硝酸根浓度呈线性关系,Nernst响应斜率为
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This one mode pays close attention to network credence foundation of the businessman very much.
这一模式非常关注商人的网络信用基础。
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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.
扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。
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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.
双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。