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LPA at different concentrations （2, 5, and 25 μmol/L） was added into the culture fluid.

在培养液中分别加入2、5、25μmol／L的LPA，为3个实验组。

L~(-1) each, 3.5μL of downstream primer 10μmol ? L~(-1,4μL of RNA,5μL of double-distilled DEPC H_2O;PCR mixture consisted of 20μL of RT-Mixture,5μL of 10 × PCR buffers, 1μL of Taq5U·μL~(-1, 5uL of mixture of dNTPs the 2.5 mmol ?

包括AMV5u·μL~(-11μL，5×Reverse transcriptase buffer 4μL，RNase Inhibitor40U·μL~(-10.5μL，dNTPs混合物10mmol·L~(-1each2μL，下游引物10μmol·L~(-13.5μL，模板4μL，水5μL；PCR反应体系：总反应体系为50μL。

Selected SMCs showed contractility stimulated by 10-5 mol/L carbachol and were recruited to endothelial lumen-like structure.

筛选到的平滑肌细胞在10-5 mol/L卡巴可刺激下出现明显收缩，且能够募集到内皮管腔样结构周围。

The nano-ionic liquid containing metal complex could be an effective catalyst for oxidative carbonylation of amines to carbamates and disubstituted ureas and for synthesis of unsymmetric ureas from amines and nitrobenzene, in which turn over frequencies exceeded 10000mol/mol/h.

以该系列纳米离子液体为催化剂高效的实现了胺类化合物催化氧化羰化制备氨基甲酸酯和二取代脲（转化频率＞10000mol/mol/h）以及胺与硝基苯通过一氧化碳羰化制备不对称脲的过程。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol／L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预，5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性；24 h后透射电镜观察细胞超微结构变化，流式细胞仪测定细胞凋亡及细胞周期的变化；5 h后Western印迹法检测细胞内IκBa的表达。

Coal is mainly physical adsorption to Cr〓(Ea=9. 245kJ·mol〓). When the concentration of waste water is comparatively low, adsorption rate to Cr〓 reaches 90% or so. The decolor rate is more than 63. 8% when coal depurates Rogor waste water. The COD removal rate is about 12% in purifying life-sewage using coal A.

煤在Cr〓溶液中对Cr〓主要为物理吸附（Ea=9.245kJ·mol〓），当Cr〓浓度较低时其去除率可达90%左右；煤处理乐果废水时脱色率高于63.8%；处理生活废水时COD去除率为12%左右。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

The results show that transition temperatures of eight wheat gluten doughs are 52.6～58.5℃; between the temperature of 0.1℃ and 40℃, apparent activation energy, which is 4602.57～6511.16 J/mol, are calculated out according to time-temperature superposition method. Fitting in both Arrhenius and WLF modes generates the identical master curves.

试验表明：面筋蛋白转变温度在52.6～58.5℃间；0.1～40℃间，频率扫描下，时间-温度叠加法算出20℃时表观活化能在4602.57～6511.16 J/mol间，不同样品间有较大的差异；Arrhenius模型和WLF模型在拟合面筋系统叠合曲线的效果上一致。

The catalytic reaction was first-order for both Ir and methyl blue with the apparent activation energy of 51.14 kJ/mol.

该催化反应对铱和甲基蓝分别为一级反应，其表现活化能为51.14 kJ/mol。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。