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The McAb against dimethoate showed certain cross reactivity for omethoate and weak cross reactivity for other several analogous compounds, and it displayed low titers and a low affinity constant. The titers of McAb in cells supernatant and ascites were 1:32 and 1:1024, respectively. The affinity constant of McAb was 1.4×10^4 L/mol.

通过杂交瘤技术，获得1株特异分泌抗乐果McAb的杂交瘤细胞，其培养上清与腹水效价分别为1：32和l：1024， McAb亲和常数为1.4×10^4L/mol，与氧化乐果的交叉反应率为10.6%，与其他所测结构类似的农药交叉反应率均小于0.5%。

The relative cross-reactivity of PA with AFB-1,ZON,T-2,FB were less than 0.01%and with an affinity constant for PA of about 1.54×10~8 liters per mol.It offered a foundation for developing immunoassay method for PA.

单克隆抗体与黄曲霉毒素B1、玉米赤霉烯酮、T-2毒素和伏马毒素B1均无交叉反应率，单抗的亲和常数为1.54x10~8 L/mol，为进一步建立青霉酸的免疫学快速检测方法提供了基础。3。

The immunological traits such as titer,affinity,sensitivity and specificity of this mAb were characterized.The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25∶1.The titer of supernatant hybridoma cell lines of 3F9-C4 was 1∶3.2×10~2 by indirect ELISA.The isotypes in ascites was IgG_(2a)/κ,the affinity constant was 8.4×10~(11)L/mol,IC50 was 8.99μg/L,cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1∶25；筛选出3F9-C4敏感特异的杂交瘤细胞1株，间接ELISA测定细胞培养上清，效价为1∶3.2×102，同种型为IgG2a/κ；腹水的亲和常数为8.4×1011L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

The immunological traits such as titer, affinity, sensitivity and specificity of this mAb were characterized. The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25:1. The titer of supernatant hybridoma cell lines of 3F9-C4 was 1:3.2×10^2 by indirect ELISA. The isotypes in ascites was IgG(subscript 2a)/k, the affinity constant was 8.4×10^11L/mol, IC50 was 8.99μg/L, cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1：25；筛选出3F9-C4敏感特异的杂交瘤细胞l株，间接ELISA 刚定细胞培养上清，效价为l：3.2×10^2，同种型为IgG(下标 2a)/k腹水的亲和常数为8.4×10^11L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

The result showed that aging time of alkali cellulose should he 60℃, the preparation condition optimized of 3-ureido-2-hydroxypropyl cellulose should be the temperature of reaction at 60℃, reaction time 3.5-4h, the mass ratio of intermediate to cellulose should be 5, NaOH concentration should be 1 mol/L.

结果表明：棉纤维的老化时间为10 h，3-脲基－2-羟基丙基纤维素的制备工艺条件以温度60℃，m（3-氯－2-羟丙基脲）／m＝5，反应时间3.5~4 h，氢氧化钠的浓度以1 mol/L为宜。

The factors which mostly affect the quality and output of the product including molar proportion of reagents, reaction temperature, alkaline concentration and reaction time are analyzed. After orthogonal experiment, it is concluded that the optimized reaction condition is that acetone and furfural are charged with equal molar quantities at 40 with sodium hydroxide (0.10 mol/L) and the operation time 5 h.

通过对影响亚糠基丙酮质量和收率的主要因素原料配比、反应温度、碱浓度、反应时间等单因素实验，探索出合成亚糠基丙酮的优化反应条件为：原料配比n：n=1.0：1.0、反应温度40℃、氢氧化钠溶液浓度为0.01 mol/L、反应时间5h。

Identification of extensive phenotypic information to 6 pare cultures and detection of the mol% G+C ratio of the DNA to representative strains, showed that the examined bacteria belonged to a new morphovar of Acinetobacter junii, and was designated as Acinetobacter junii Morphovar Ⅰ. In addition, serotype, antibiotic sensitivity and pathogenicity of isolates were studied, the results showed that the 6 strains have the same K-antigen and O-antigen, there arc no obvious differences in sensitivity and resistance to used 37 antimicrobial agents between strains, and have strong pathogenicity to experimental stone bonder and Bastard halibut.

经对6株纯培养菌在形态特征、理化特性等方面较系统的表观分类学指征鉴定及代表菌株DNA中G+C mol%的测定，表明为琼氏不动杆菌的一个新形态型并定名为琼氏不动杆菌形态型Ⅰ(Acinetobacter junii morphovar Ⅰ)；同时，对该菌进行了血清型、对抗菌类药物的敏感性及致病作用等方面的试验，初步表明此6株菌具有同种的表面及同种的菌体抗原，对供试37种抗菌类药物在不同菌株间的敏感及耐药无明显差异，对供试石鲽及牙鲆均具有较强的致病作用。

When the Eu(superscript 2+) concentration is 0.005 mol^(-1), the sample presents intense blue-white emitting.

当Eu(上标 2+)浓度为0.005 mol^(-1)时，样品呈现很亮的蓝白色发光。

RESULTS: We got the structures of 50 new chemical compounds as the lead compounds for further drug filtering, and 3 of 8 compunds we synthesized successfully had a bioactivity between 220 and 310 μmol/L.

结果：得到50种化合物结构并成功合成8种，经测定其中3种有活性（IC50在220~310 μmol/L间）。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。