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The methodological 2 oxidation 75 μ Mol/L are cobaltic alone or jointly 30, the Rg3 action of 60 μ G/m L is mixed at 24 H of cell of esophagus cancer EC9706 48 H, part fluid of the Qing Dynasty on collection cell education, detect with ELISA law among them hemal endodermis grows factor level, in order to contain RPM I1640 of serum of 10% embryoes ox education radical does blank to contrast.

方法将75μmol/L的二氧化钴单独或联合30、60μg/m l的Rg3功能于食管癌EC9706细胞24 h和48 h，分别收取细胞培养上清液，用ELISA法检测其中血管内皮生长因子水平，以含10%胎牛血清RPM I1640培养基做空白对照。

The type of cell death caused by ricin was related to the ricin concentration and time of incubation with HeLa cells. The typical apoptosis was induced by ricin 0.05μmol〓. The apoptotic cells mainly showed cell shrinkage, cytoplasmic membrane blebbing, the chromatin condensation and fragmentation, crescentic nuclear and membrane bound apoptotic bodies formation.

细胞死亡方式也与蓖麻毒素作用时间和浓度相关，0.05μmol.L〓的蓖麻毒素可引起HeLa细胞发生典型的细胞凋亡，主要特征是细胞体积缩小，胞浆起泡，细胞核染色质浓缩、凝集，新月状核和膜包裹核染色质的凋亡小体形成。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

The overall activation energies of crotonic acid, undecylenic acid and oleic acid systems are 118, 122 and 134 kJ/mol, respectively.

反式丁烯酸、10-十一烯酸、油酸接枝ABS树脂的反应活化能分别为：118、122、134kJ／mol。

Under the optimum conditions of a cyclopentene amount of 1.0 mol, a molar ratio of cyclopentene to water of 0.25, a reaction temperature of 120 ℃, a reaction time of 4.0 h, a main catalyst dosage of 4.0 g and a cocatalyst dosage of 0.8 g, the yield of cyclopentanol reached 68.6%.

确定适宜的反应条件为：环戊烯1.0 mol，n﹕n=1﹕4，反应温度120 ℃，反应时间4.0 h，硅胶负载铌酸主催化剂用量4.0 g，三（3，6-二氧杂庚基）胺助催化剂0.8 g。在此反应条件下，环戊醇收率68.6%。

Methods: In alkaline condition, reaction between amino acids and phenylisothiocyanate formed a derivant of phenylisothiocyanate-amino acid. For RP-HPLC, the Diamonsil C18 column 250 mm×4.6 mm,5 μm） and binary gradient elution were used. The mobile phase A was 0.1 mol/L sodium acetate solution-acetonitrile (93∶7） and the mobile phase B was acetonitrile-water（80∶20）. The detecting wavelength was 254 nm.

在碱性条件下，氨基酸与异硫氰酸苯酯反应，生成异硫氰酸苯–氨基酸的衍生物，采用 Diamonsil C18色谱柱（250 mm×4.6 mm，5 μm），用二元梯度洗脱方式，流动相A为0.1 mol/L乙酸钠–乙腈（93∶7），流动相B为乙腈–水（80∶20），检测波长254 nm。

Urinary sample was acidified by 2 mol/L hydrochloric acid and pretreated by liquid-liquid extraction using diethyl ether.

尿样经2 mol/L盐酸酸化后用乙酸乙酯进行萃取。

Pack Phenyl column, with 0.005 mol/L potassium dihydrogen phosphate(pH 6.5)????

Pack苯基柱，用0.005 mol/L磷酸二氢钾(pH 6.5)？？？？

The dilative effects of acetylcholine on rat aorta precontracted with NE 0.62 μmol/L were investigated to reflect the changes of dilative functions.

用去甲肾上腺素诱发大鼠离体主动脉环的收缩，反映收缩功能的变化，主动脉环以0.62 μmol/L NE预收缩后用乙酰胆碱舒张对抗，反映舒张功能的变化。

Using orthogonal experimental design with orthogonal layout of L16(45), effects of cadmium(0,100,1000,2000μmol/L) and estrous cycle(proestrous,estrous, postestrous, dioestrous)on steroidogenesis in the ovary were studied.

采用正交表L16(45)设计正交实验，研究镉(0、100、1000、2000μmol/L)和动情周期(动情前期、动情期、动情后期、动情间期)对卵巢切碎组织分泌E2和P4的影响。3。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。