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The optimum conditions are 0.2 mol chlorobenzene, and 0.7 g catalyst, and 0.07 mol glycine, and 0.08 mol nitric acid, and reaction time of 3 hours, and reaction temperature of 45℃.

最宜工艺条件:氯苯为0.2 mol,催化剂为0.7 g,氨基乙酸为0.07 mol,硝酸为0.08 mol,反应温度为45℃,反应时间为3 h。

Optimum extraction conditions were selected by the condition experiments and the concentration of Cu, Pb, Zn in main components were also determined for revealing the distribution of the heavy metals in the suspended particulate matters. It was found that hydroxylamine hydrochloride (0.006 mol/L NH2OH·HCl+0.01 mol/L HNO3) can remove most of Mn oxides, and sodium dithionite 0.8 mol/L Na2S2O4

结果表明,0.006 mol/L NH2OH·HCl+0.01 mol/L HNO3萃取30 min可以单独萃取悬浮颗粒物中大部分的锰氧化物,0.8 mol/L Na2S2O4(pH=6.0)萃取40 min可以同时萃取铁、锰氧化物,而对非目的组分的影响较小;悬浮颗粒物对Cu、Pb、Zn重金属有明显的富集作用,其中Cu主要结合在悬浮颗粒物的有机质组分中,Pb主要富集在铁、锰氧化物中,锰氧化物和有机质对Zn的分布起主要的富集作用。

METHODS: The rabbit hindlimb normothermic tourniquet ischemia model was employed. The tendon on the dorsum of the foot was exposed for observation of microvessels. The responses of arterioles on tendon surface to topical application of 10(-6) mol/L noradrenaline, 10(-6) mol/l acetylcholine (an endothelium-dependent vasodilator) and 10(-4) mol/L nitroglycerin (an endothelium-independent vasodilator) were observed at the period of ischemia and following 30 minutes of reperfusion after 2 hours and 5 hours of ischemia by use of intravital microscopy.

应用兔后肢止血带常温缺血模型及足背肌腱表面微循环观察法,观察肢体缺血 2小时及 5小时再灌注后微动脉对局部滴注血管收缩剂 [去甲肾上腺素(10 - 6 mol/ L ) ]、内皮依赖性血管舒张剂 [乙酰胆碱(10 - 6 mol/ L ) ]及内皮非依赖性血管舒张剂 [硝酸甘油(10 - 4 mol/ L) ]的反应性变化。

Methods The cells were divided into 5 treatment groups(10,25,50,75 and 100 μmol·L-1 QUE), blank control and menstruum control group. The rat C6 cells were cultivated to 1×106·mL-1 in the RPMI 1640 medium, then added into 96 holes board with various doses of QUE by 3 holes per group,and MTT assay was used to observe the proliferation of the cells treated for 24,48 and 72 h. The change of cell cycle was also observed by flow cytometry after the cells were treated with 50 and 100 μmol·L-1 QUE for 48 h. The changes of the protein P53 and Bcl-2 of C6 cells treated with 50 μmol·L-1 QUE for 48 h were detected by immunocytochemical methods.

按QUE浓度分成10、25、50、75及100 μmol·L-15个处理组和空白对照组及溶剂对照组,大鼠脑胶质瘤C6细胞在RPMI 1640培养基中生长达1×106·mL-1后,在96孔板中分别加入上述浓度的QUE继续培养,每组设3复孔,作用24、48及72 h,采用MTT比色法检测QUE对大鼠脑胶质瘤C6细胞的增殖抑制情况,流式细胞术对50及100 μmol·L-1的QUE作用48 h的大鼠脑胶质瘤C6细胞进行周期分析,免疫组化法检测50 μmol·L-1的QUE作用48 h 的p53和bcl-2基因产物。

Methods Synaptosomes was made from hippocampus and incubated with artificial cerebrospinal fluid. 30μmol/L propofol, 50μmol/L picrotoxin or both was added into aCSF. Intralipid was administered as solvent control and any drugs was applied in control. To observe Ca~(2+)-dependent release of glutamate and GABA, dihydrokainic acid and nipectic acid were added into aCSF.

制备大鼠海马突触体,随机分为5组:PTX组加入印防己毒素使其终浓度为50μmol/L,Pro30+PTX组加入终浓度为30μmol/L的丙泊酚和50μmol/L的PTX,Pro30组加入终浓度为30μmol/L的丙泊酚,溶剂对照组加入脂肪乳,空白对照组不加入上述药物。

The linear range for resorcinol is 5.0×10^(-9)~1.0×10^(-4) mol/L with the detection limit of 3.0×10^(-9) mol/L. The relative standard deviation is 3.5% for 1.0×10^(-6) mol/L resorcinol in 11 repeated measurements.

该法测定间苯二酚的线性范围为5.0×10^(-9)~1.0×10^(-4)mol/L,检出限为3.0×10^(-9)mol/L,相对标准偏差为35%(1.0×10^(-6)mol/L间苯二酚,n=11。

Results ①Arsenicals caused inhibition obviously on HL-60 cells growth at the test concentrations,particularly in the groups of 2μmol/L,10μmol/L sodium arsenite,and the groups of 100 μmol/L,300 μmol/L sodium arsenate.

结果①两种无机砷化物对HL-60细胞生长具有抑制作用,特别是高浓度NaAsO2(2、10 μmol/L)和Na3AsO4(100、300 μmol/L)可明显地抑制HL-60细胞的生长

The light saturate point and light compensate point of seedling were 1841.67nmol m-2s-1 and 155.18 mol m-2s-1 respectively, but the LSP and LCP of the young trees were 1810.00 m-2s-1 and307.07 mol m-2s-1.(3) Comparing with other three tree species, the tree of heaven had special leaf POD isozyme patterns related with the characteristic of insect-resistance.

幼苗、幼树叶片的净光合速率日变化具有两种变化曲线,均出现"午休"现象:幼苗的光饱和点与光补偿点分另伪1841.67μmol·m~(-2)·s~(-1)和155.18μmol·m~(-2)·s~(-1);幼树的LSP与LCP分别为1810.00μmol·m~(-2)·s~(-1)与307.07μmol·m~(-2)·s~(-1)。

Under technological conditions such as: room temperature, current density 200 A/m^2, anolyte acidity 0.5 mol/L chlorhydric acid, acidity of catholyte 1.5 mol/L chlorhydric acid, circulation of anolyte and catholyte 40 L/h, Ni(superscript 2+) concertration 0.85 mol/L, and the average cell voltage 1.3 V, the electricity consumption of dissolving a ton of nickel base alloy is 813 kWh, and the Ni(superscript 2+) retention rate of the ion exchange membrane is more than 99.5%.

在电流密度200 A/平方公尺、阳极酸度0.5 mol/L、阴极酸度1.5 mol/L、循环量40 L/h、Ni(上标 2+)浓度0.85 mol/L左右和室温的条件下电解,平均槽电压1.3 V,电溶1 t镍合金板耗电813 kWh,阴离子交换膜对镍的截留率大於99.5%。

PKC and PKA activity assays showed that 0.03μmol/L of 20-HETE increased PKC and PKA activity in isolated cardiomyocytes, while chelerythrine (5μmol/L) and H-89 (5μmol/L) inhibited these effects.

PKC和PKA活性测定的结果表明20-HETE(0.03μmol/L)增加心肌细胞PKC和PKA的活性,并且白屈菜赤碱(5μmol/L)和H-89(5μmol/L)抑制该效应。

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