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To explore the mechanisms of lead-induced apoptosis, PC 12 cells were exposed to 100 μmol·L-1 lead acetate for 24 h, and cellular morphology, cytoskeleton and ultrastructure were observed.

为了从形态学角度研究铅诱导细胞凋亡的机制,用100 μmol·L-1乙酸铅对PC12细胞进行染毒24 h,检测细胞整体形态、细胞骨架和细胞超微结构的改变。

The flow of interstitial cations to normal sites cannot, however, continue indefinitely, but should reachequi librium and cease at a particular dopant-concentration (possibly at around10-4 mol % Ba2+).

人流间阳离子正常用地可以,但不能无限期地持续下去,而应reachequi librium并停止在某一特定掺杂浓度(可能在around10 - 4 mol %时的Ba2 +)。

The peak current at about -0.90V showed a linear dependence with the concentration of isoniazid over the range 1.2×10^(-10) and 1.3×10^(-3)mol/L.

发现在-0.90V出现的还原峰的峰电流与异烟肼的浓度在1.2×10^(-10)~1.3×10^(-3)mol/L的范围内成线性关系。

Moreover, the catalytic currents present linear dependence on the concentration of hydrazine from 2.5μmol·L~(-1) to 0.2 mmol·L~(-1) by amperometry.

安培法测得催化峰电流与甘氨酸浓度在20μmol·L~(-1)-1.0 mmol·L~(-1)范围内呈线性关系。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0,5.0,10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理,设空白对照组,分别孵育24h、48h、72h后,收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化;免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

Choline and folic acid deficiencies could induce cell death in human lymphoblast cell line, and 6 μmol/L CC and 120 nmol/L FA was the optimal combination for keeping the lowest level of APO and NEC in human lymphoblast cell line.

胆碱与叶酸不足可诱导人成淋巴细胞株细胞死亡,6 μmol/L氯化胆碱及120 nmol/L叶酸是维持体外培养的人成淋巴细胞株APO及NEC频率在最低水平的最适组合浓度。

The results showed that molecular weight of malate dehydrogenase was 69 kDa which included two subunits with the molecular weight was about 34 kDa. The optimal pH, temperature and Km were 8.0, 50℃ and 84. 6μmol/L (oxaloacetic acid, pH 8.3), respectively.

结果表明:苹果酸脱氮酶的相对分子质量约为69000,含有2个亚基,每个亚基的相对分子质量约为34000;该酶的最适作用pH为8.0,最适作用温度为50℃;K值为84.6μmol/L(草酰乙酸,pH8.3)。

The method uses Chinese pharmacopoeia 2005 edition dissolve of 2 appendices XC goes out degree determine the first law, it is menstruum with 0.1 Mol.L-1 hydrochloric acid, rotate speed is 75 R.min-1, sampling time is 30 Min, determine in good qualities of 287 Nm wave its dissolve goes out degree.

方法采用中国药典2005年版二部附录XC溶出度测定第一法,以0.1 mol.L-1盐酸为溶媒,转速为75 r.min-1,取样时间为30 min,在287 nm波优点测定其溶出度。

01 Mol/L solution of sodium heptanesulfonate (to adjust PH to 2.2 with the debited orthophosphoric acid)-methyl cyanide (44:56) as mobile phase, detective wavelength was 254 nm.

采用HPLC法以辛基硅烷键合硅胶为填充剂;以0.01 mol/L庚烷磺酸钠溶液(稀磷酸调PH值至2.2)-乙腈(44:56)为流动相;检测波长为254 nm。

Action potentials were recorded using a conventional glass microelectrode filled with 3 mol/L KCl solution.

分离豚鼠乳头肌的单个心室肌细胞,采用内充3 mol/L KCl的玻璃微电极记录心肌动作电位。

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I this is at that time, the opinion with peacockish true girl is full of in a heart.

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