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DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

The activation energy of cellular decomposition calculated by DSC date was 88.24kJ/mol, which showed that the decomposition was controlled by Zn atom diffusion.

DSC数据计算得出，胞状分解的相变激活能为88.24kJ/mol，表明其分解主要受锌原子扩散控制。

The 〓 of the used reverse micelles was optimized.The results demonstrated that the isolated yield could reach 86% when 〓=6, 400μmol/1 α-chymotrypsin in the water pool and stirring for 24 hours.

实验结果表明，当反相胶束体系的〓值为6，反应时间为24h，内核水池中酶的浓度为400μmol／1时，反应产率可以达到86％。

The results show that the flow stress decreases with increasing deforming temperature and increases with increasing strain rate. The flow stress of the alloy during hot compression deformation can be described by constitutive equation in hyperbolic sine function with a hot deformation activation energy of 196.27 kJ/mol. Partial recystallization takes place in the alloys deformed at a high temperature or at a low strain rate, and large number of dislocations and subgrains are observed in the alloy. The elongated grains observed in the samples coarsen with increasing temperature and decreasing strain rate. Correspondingly, the subgrain size increases and the dislocation density decreases. The main soften mechanism of the alloy transforms from dynamic recovery to dynamic recrystallization.

结果表明：Al-Cu-Mg-Ag耐热铝合金在热压缩变形中的流变应力随着温度的升高而减小，随着应变速率的增大而增大；该合金的热压缩变形流变应力行为可用双曲正弦形式的本构方程来描述，其变形激活能为196.27kJ/mol；在变形温度较高或应变速率较低的合金中发生部分再结晶，并且在合金组织中存在大量的位错和亚晶；随着温度的升高和应变速率的降低，合金中拉长的晶粒发生粗化，亚晶尺寸增大，位错密度减小，合金的主要软化机制逐步由动态回复转变为动态再结晶。

In a medium with pH 9.0 DBQADHP reacts with cobalt to form a green complex,exhibiting maximum absorbance at 670 nm,and the molar absorptivity is 7.6×104 L·mol-1·cm-1. Beers law is obeyed for 0～6 μg of cabalt in 25 mL solution.

实验结果表明：在pH9.0的缓冲体系中，显色剂与钴发生灵敏显色反应，形成绿色配合物，其最大吸收波长位于670nm，表观摩尔吸光系数为7.6×104L·mol-1·cm-1，钴量在0～6μg/25mL范围内遵守比尔定律。

TFP and CPZ, two CaM antagonists, could inhibit growth of coleoptile and root at higher concentration(≥50 μmol/L), and enhance the activity of peroxidase.

一定浓度(≥ 5 0 μmol/L)的CaM拮抗剂三氟拉嗪和氯丙嗪均抑制小麦胚芽鞘及根系的生长，提高过氧化物酶活性。

Results show that fabric first treated with 2% silica, followed immersed in 0.3×10^(-3)mol/L Ag(superscript +) solution for 30 minutes can inhibit 99% colon bacillus even after 50 laundering cycles.

结果表明，硅化物浓度2%、Ag浓度0.3×10^(-3)mol/L、浸渍时间30min的抗菌效果最佳；织物经50次洗涤，其大肠杆菌减少百分率仍在99%以上。

Many-body analysis was also carried out to obtain relaxation energy and many-body interaction energy (two, three, and four-body), and the most stable conformer has the basis set superposition error corrected interaction energy of －102.61 kJ/mol.

多体相互作用分析表明，最稳定的氢键结构经基组重叠误差校正后总相互作用能为-102.61 kJ/mol，驰豫能，两体与三体相互作用能对总相互作用能的贡献较大，四体相互作用能对总相互作用能有很小的贡献。

The total inter-action energy of the most stable conformer has the basis set superposition error corrected total interaction energy 99.53 kJ/mol, and many-body analysis shows that two-and three-body energies have significant contribution to the total interaction energy whereas the relaxation energies and four-body energies are small.

对8个稳定氢键结构进行多体相互作用分析表明，最稳定的氢键结构经基组重叠误差校正后总相互作用能为－99.53kJ/mol，两体与三体相互作用能对总相互作用能的贡献较大，驰豫能与四体相互作用能对总相互作用能有很小的贡献。

5 And 10 μmol/L teams and every team was subdivided into experiment group and control group.

将脐静脉内皮细胞分为0.5、1、5、10μmol/L组，各组再分为实验组与对照组。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。