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Methods This study contained a total of 16 culture groups, including one group as control and 5 groups treated by 5 drugs (calcium L-threonate, sodium L-threonate, alendronate, 17β-estradiol and calcium gluconate) each at the final concentrations of 10^(-9)mol/L, 10^(-7)mol/L, 10^(-5)mo1/L respectively. After 7 days, eight bone slices of every group were stained with toluidine blue and the areas of resorptive pits were analyzed under light microscope; the concentrations of C-telopeptide of type Ⅰ collagen CT(subscript x or Crosslaps in culture supernatants were measured by ELISA.

制备骨磨片，培养OC，并分别加入高中低浓度10^(-5、10^(-7)、10^(-9)mol/L的L-苏糖酸钙、L-苏糖酸钠、阿仑麟酸钠、17β-雌二醇、葡萄糖酸钙，实验设以上各浓度组及对照组（共16组，每组n=8），甲苯胺蓝染色一光镜观察分析骨片上骨吸收陷窝面积，ELISA测定上清液Ⅰ型胶原C-末端肽（CTx或Crosslaps）浓度。

Under inverted microscope, stereognosis of neurons died down, there were blank bulb in the neurons, dark particle were seen in the neuritis; the result of MTT test showed that the survival rate of neurons was obviously decreased. Res 40μmol/L increased the survival rate of neurons. Aβ(subscript 25~35) dose-dependently increased the level of MDA, decreased the level of GSH. Res dose-dependently increased the level of GSH, decreased the level of MDA.

结果：Aβ(下标 25~35)在终浓度为2μmol/L时神经元形态有明显的变化，细胞立体感减弱，胞体内空泡形成，细胞突起内可见黑色颗粒状物；细胞的存活能力明显下降，与对照组相比较有显著性差异(P.05)；Res在终浓度40μmol/L时，能明显提高细胞的存活能力。Aβ(下标 25~35)可以明显升高细胞培养上清液中MDA含量，降低GSH的含量；Res可使细胞培养上清液中MDA含量下降，GSH含量增加。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明，处理后4周，全周角膜缘上皮板层手术切除，中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化，未发生睑球粘连，角膜基质胶原纤维完整未见溃疡、穿孔等病变，细胞印迹学检查为结膜表型，可作为实验性角膜缘干细胞移植的病理模型；全周角膜缘上皮板层手术切除，中央角膜上皮用生理盐水擦除的5只试验家兔，有2只为结膜表型，另3只为角膜表型，观察期内结果不稳定；半周角膜缘上皮板层手术切除，中央角膜上皮层用生理盐水擦除的5只试验家兔，角膜表面透明，全部为角膜表型；直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔，有4只角膜基质胶原纤维断裂、溶解，并伴有严重的溃疡、穿孔、睑球粘连等病变，不能用于移植试验，另1只角膜表面透明，未见结膜和新生血管长入，细胞印迹学检查为角膜表型。

Using Eriochome cyanine R staining, we found that Al accumulation on surface of root tips was positive related to the inhibition of root growth in treatments of 0 - 80μmol·L~(-1) Al for 24 h. Exchangeable Al in apoplast and symplasm of root tips of wheat was effectively desorbed by 5 mmol·L~(-1) CaCl_2 at 4℃.

铬化青R染色可以原位显示0-80μmol·L~(-1)Al处理后根尖表面Al的吸附量与根尖伸长受抑制程度具有很好的一致性。5mmol·L~(-1) CaCl_2溶液可以有效洗脱10和40μmol·L~(-1)Al处理24小时后小麦根尖质外体和共质体中可交换态Al。

The neonatal Wistar rats, clean degree, were used. The hippocampal neuron cells out of 9 rats were cultured and divided into the control groups of before and after hypoxia-oxygenation,and xionggui diwan 1 × 10-6 mol/L and 10 × 10-6 mol/L groups. Xionggui diwan was provided by the 88 Hospital of Chinese PLA and its active ingredient was the volatile ingredient of chuanxiong and danggui.

选择新生清洁级Wistar大鼠1只，取其海马，将其分离培养成海马神经元细胞，然后选取9例海马神经元细胞，将其放入3个培养皿中培养，每个培养皿中3例神经元细胞，将其分为缺氧-复氧前后、芎归滴丸(由解放军第八十八医院制剂室提供，主要成分为川芎和当归的挥发油)1×10-6mol/L组和芎归滴丸10×10-6 mol/L组。

Dilatiry of AS on rat thoracic aorta strips precontracted by noradremaline（1μmol/L）and KCl（30mmol/L）were proved.

结果 AS松弛去甲肾上腺素（1μmol/L）和氯化钾（30mmol/L）预收缩的大鼠胸主动脉条，其EC 50 分别（35.9±3.1）和（36.7±3.1）μmol/L。

The oligomeric states of PSⅡcomplex in soybean leaves treated with moderate light (700μmol〓) and strong light (2000μmol〓) were studied by nondenaturing PAGE and gel filtration chromatography. The following results were obtained. PSⅡ dimers resolved by nondenaturing PAGE accounted for about 75% of total PSⅡ complexes and there was no significant difference in the ratio of PSⅡ dimer to monomer between samples from moderate light-treated and fully dark-adapted leaves.

利用非变性聚丙烯酰胺凝胶电泳方法分离PSⅡ双体和单体，结果表明PSⅡ双体约占总PSⅡ含量的75％，并且经中等光（700μmol m〓）处理后，大豆叶片PSⅡ单体/双体比例没有明显变化。

These results show that the ring expansion of cyclopropylsilylene C3H5SiH to silacyclobutene is easy to occur with a barrier of 113.4kJ/mol, while the 1,2-H shift is not competitive to the ring expansion for its much higher barrier.

结果表明，环丙基硅烯经过113.4kJ/mol的势垒扩环重排为硅杂环丁烯为自发反应；而其1，2-氢迁移重排反应热垒为190.0kJ/mol，是非自发反应，难于进行，不能与扩环重排相竞争。

RESULTS: Luteolin was proved to inhibit strongly the holoenzyme activity of recombinant human protein kinase CK2 with IC50 of 0.86 μmol/L.

酶动力学分析表明，藤黄菌素与ATP呈竞争性抑制CK2的活性（Ki=0.19 μmol/L），与酪蛋白则呈混合性抑制CK2的活性（Ki=0.11 μmol/L）。

In darkness, the Ca〓-ionophore A23187 could mimic the effect of R, the volume and 〓 accumulation in protoplasts were 82. 91% and 90.62% respectively as those of R treatment when 5μmol/L A23187 was added to the medium.

在黑暗条件下，钙离子载体A23187（1～10μmol/L）可模拟红光的作用，5μmol/L A23187使原生质体膨大程度达红光处理的82.9l％，〓积累量达红光处理的90.62％。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。