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And the low frequency vibrations contribution from ring A and oxygens to the internal reorganization energy of MQ〓(n=1～7) is greater than 1.60kJ.mol〓 in addition, the low frequency vibrations contribution from the isoamylene substituent to the internal reorganization energy of MQ〓 and MQ〓 is about 2.0kJ〓.

得电子前后，环A及对位氧的二面角变化对质体醌MQ〓(n=1～7)的内重组能的贡献大于1.60kJ.mol〓，而且得电子前后，异戊烯支链结构参数的变化尤其是二面角的变化对MQ〓／MQ〓及 MQ〓/MQ〓体系的内重组能贡献约为2.0kJ-mol〓。

Results: When the concentrations of artemisinin were 1×10^(-4), 1×10^(-5) and 1×10^(-6)mol/L, the growth of cells was inhibited remarkably in a dose-dependent manner. Fluorescence staining showed obvious apoptosis, such as karyopyknosis and agglutination. RT-PCR assay shows the expression of Caspase-3. Artemisinin could lead to the decrease of mitochondrial cytochrome c concentration, which in turn lead to the increase of cytoplasmic cytochrome c concentration.

结果：青蒿素的浓度为1×10^(-4)，1×10^(-5)，1×10^(-6)mol/L时，细胞生长受到显著抑制，并呈剂量依赖性；Hoechst33342/PI双荧光染色可观察到明显的核浓缩、凝集等细胞凋亡表现；RT-PCR检测到Caspase-3的表达；Western-blot检测1×10^(-5)mol/L药物处理细胞后线粒体细胞色素C表达水平下调，细胞浆出现明显细胞色素C蛋白条带。

This was done using a high-performance liquid chromatography system equipped with an electrochemical detector, endonuclease nicking assay and reverse transcription polymerase chain reaction, respectively. RESULTS: H2O2 induced the formation of 8-oxo-dG in DNA at concentrations of 100 and 200 μmol/L.

结果：与对照组比较，100、200、300μmol/LH2O2浓度时均能使A549细胞DNA的8-oxo-dG水平增高（P.05或P.01）。200μmol/LH2O2作用12h后8-oxo-dG水平上升到峰值并在24h降至基线水平，8-oxo-dG水平在6~18h处理组显著高于对照组（P.05或P.01）。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases.

三级指数拟合表明：细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；细胞外Ba 2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases. CONCLUSION: Two diffe rent mechanisms for external Ba2+ blocking exist.

三级指数拟合表明：细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；细胞外Ba2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases. CONCLUSION: Two diffe rent mechanisms for external Ba2+ blocking exist.

三级指数拟合表明：情绪细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；情绪细胞外Ba2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

The results showed that SPR angel changed very little when the concentration of phenanthroline or iron changed from 10~(-6) mol/L to 10~(-4) mol/L, but the combination of phenanthroline with iron produced great SPR response.

结果表明，在10~(-6)～10~(-4)mol／L浓度范围内，铁和邻二氮菲各自SPR角度变化较小，而它们的络合物有较大的SPR响应，据此进行它们的相互作用研究。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

The colon temperatures were measured by a thermistor and the cytosolic free calcium concentration ([Ca2+]i) in dissociated brain cells was measured by Fura 2-AM. The results demonstrated that the pyretic action of egtazic acid (0.6 μmol, icv) was markedly inhibited by DEX (5 mg/kg,iv), but DEX (60-120 μmol/L) did′t affect [Ca2+]i in dissociated hypothalamus cells. Actinomycin D, which interferes with gene transcription (3 nmol, icv), completely abolished the antipyretic action of DEX on egtazic acid-induced fever.

结果发现：脑室灌注乙二醇双(2-氨基乙醚)四乙酸(0.6 nmol)引起家兔结肠温度明显升高，静脉注射地塞米松(5 mg/kg)显著抑制家兔乙二醇双(2-氨基乙醚)四乙酸性发热，地塞米松(60-120 μmol/L)并不影响下丘脑细胞内[Ca2+]i，而事先脑室灌注抑制基因转录的放线菌素D(3 nmol)则完全取消了地塞米松对乙二醇双(2-氨基乙醚)四乙酸性发热的解热作用。

Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG. The solubility of inclusion bodies of rCPB in 10mol/L urea was 2~3 times higher than in 8 mol/L urea, and the renature solution with 0.75%β-ME in 10mol/L urea could increase the solubility of inclusion bodies of rCPB, comparing with that in 10mol/L urea simply.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响；rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍；添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。