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Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

The result indicated, In the MS culture medium, and the damagement increased with Pb(superscript 2+) concentrition, when Pb(superscript 2+) concentration was up to 1000μmol/L, the callus was geatly damaged and its character is small, hard, skin drying; Organic acid of suitable concentration was able to improve the induction and growth of rice callus under the Pb(superscript 2+) stress, i.e. ascorbic acid, critic acid and oxalic acid The effect was the best which added 750 mol/L citric acid in MS culture medium, the callus was fresh, light yellow, loose, the surface moist. and survival was as higher as controlled treatment.

而添加不同有机酸（抗坏血酸、柠檬酸和草酸）均可明显改善Pb(上标 2+)胁迫下愈伤组织的生长，尤以750μmol/L柠檬酸对Pb(上标 2+)毒害的缓解效果最佳，其愈伤组织块颜色浅黄，表面湿润，愈伤组织成活率高，与对照愈伤组形态较接近。

More than 75%of dodecene,decene,allylplenylether,phenylpropene(1.9×10－3mol) were completely hydrosilylated with 23.2 mg complex containing 4.9×10－6 mol platinum within 0.75 hour.

使用23.2 mg络合物(含4.9×10－6 mol Pt)为催化剂、分别使用1.9×10－3 mol 1－十二烯、癸烯、烯丙基苯基醚、苯丙烯进行硅氢加成反应，在0.75 h内产率超过75%。

The results showed that resveratrol dose dependently inhibited the expression of these two genes in a concentration range from 5μmol/L to 50μmol/L.

结果表明白藜芦醇在5~50μmol/L的浓度范围能显著的抑制TNF-α和iNOS的表达，白藜芦醇的上述抑制作用呈浓度依赖性。

NADPH oxidase inhibitor, diphenylene iodonium chloride (DPI, 10 μmol/L), abolished histamine-stimulated calcium oscillations both in normoxic and posthypoxic PAECs.(3) Xanthine oxidase inhibitor, oxypurinol (100 μmol/L), did not affect the calcium oscillation kequency in normoxic PAECs. However, it significantly decreased the elevation of calcium oscillation frequency in posthypoxic PAECs.

黄嘌呤氧化酶抑制剂，别嘌呤醇(oxypurinol，100 μmol/L)能显著降低组胺刺激的缺氧后肺动脉内皮细胞升高的钙振荡频率，但降低后的钙振荡频率仍高于常氧组，别嘌呤醇对组胺刺激的常氧组肺动脉内皮细胞钙振荡频率无显著影响。

NADPH oxidase inhibitor, diphenylene iodonium chloride (DPI, 10μmol/L), abolished histamine-stimulated calcium oscillations both in normoxic and posthypoxic PAECs.(3) Xanthine oxidase inhibitor, oxypurinol (100μmol/L), did not affect the calcium oscillation frequency in normoxic PAECs. However, it significantly decreased the elevation of calcium oscillation frequency in posthypoxic PAECs.

黄嘌呤氧化酶抑制剂，别嘌呤醇(oxypurinol， 100μmol/L)能显著降低组胺刺激的缺氧后肺动脉内皮细胞升高的钙振荡频率，但降低后的钙振荡频率仍高于常氧组，别嘌呤醇对组胺刺激的常氧组肺动脉内皮细胞钙振荡频率无显著影响。

The Ea for the formation of ethoxide intermediate was 170.34 kJ/mol. Alkylation of benzene with ethylene occurred through both concerted mechanism and stepwise mechanism, and they were competitive to each other. These two paths were only slightly different in the activation energy.

而乙烯作为烷基化剂与苯反应时同时存在联合机理和分步机理，且二者之间存在一定程度的竞争，其中联合机理的活化能为167.24 kJ/mol，分步机理速控步骤的活化能为155.20 kJ/mol。

Preliminary antitumor activity in vitro of the new compounds indicated that the compounds have obvious inhibitory effect on human leukaemia cell (HL-60) and human galactophore cancer cell (MDA-MB-468) in the concentration of 10-4 mol/L. This result is a basis for further study on the synthesis and biological activity of this kind of compounds.

对所合成的10个新化合物进行了抗肿瘤体外生物活性试验，初步生物活性实验结果表明，该类化合物在浓度为10-4 mol/L时对人白血病细胞株(HL-60)和人乳腺癌细胞株(MDA-MB-468)有明显的抑制作用，在浓度为10-5 mol/L时没有明显的抑制作用，这一研究结果为进一步研究此类化合物的合成、生物活性的研究奠定了基础。

It is shown that the reaction U+CO→CUO with a long-lived intermediate complex has no threshold energy, which agrees with the potenlial surface. When the collision energy is greater than 418.4 kJ/mol, the intercharge reaction increases, until the CUO molecule will decompose into U, C, O completely, and these reactions have threshold energy.

结果表明：在碰撞能低(15kJ/mol)时可以生成长寿命络合物CUO(X^3A)，并且该络合反应是无阈能反应；碰撞能大于418.4kJ/mol后，先后出现置换产物UO和UC；随着碰撞能进一步增大，CUO分子将被完全碰散成U，C和O原子，而且反应U+CO(0， 0)→UO+C，U+CO(0， 0)→UC+O和U+CO(0， 0)→U+O+C是有阈能反应。

RESULTS:Isoalantolactone exhibited excellent anti-proliferative activities on HeLa, SHIN3 and HOC-21 and HAC-2 cell lines, while 1-O-acetylbritannilactone and britannilactone demonstrated weak anti-proliferative activities on HeLa, SHIN3, HOAC-21 and HAC-2 cell lines even at the concentration of 100 μmol/L. The apoptotic rate in isoalantolactone group with the concentration of 12.5 μmol/L was higher than that in PBS group.

结果： 异土木香内酯对上述5种肿瘤细胞的增殖均有明显的抑制作用，1-氧-乙酰大花旋覆花内酯和大花旋覆花内酯即使在100 μmol/L的高浓度下，对5种妇科肿瘤细胞的增殖不显示抑制活性；流式细胞学检查发现异土木香内脂处理后的HeLa细胞出现凋亡现象。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。