查询词典 model t

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

The time of cornea diabrosis, metastases into cervical lymph nodes and lung, survival time and ratio of CD4+ and CD8+ T cells in peripheral blood were observed.RESULTS: The time of cornea diabrosis in GTVC group or GTV group was later than that in PBS group, but no statistical difference between GTVC group and GTV group. Metastatic incidence in cervical lymph nodes of GTVC group mice or GTV group mice was lower than that in PBS group 18 days after the model had been established. There was no statistical difference in metastatic incidence between GTV group and GTVC group.

将荷瘤模型鼠分为磷酸盐缓冲液（phosphatebuffered saline，PBS）治疗组，IL12基因修饰肿瘤细胞疫苗（gene modified tumor cell vaccine， GTV）治疗组及GTV 联合CpGODN治疗组；鼠荷瘤模型建立后的第3d及第10d分别在各组鼠结膜下注射30μL PBS，30μL（3.0×106个细胞）灭活的GTV及含20μg CpGODN灭活的GTV，进行角膜溃破时间、淋巴结和肺部转移瘤发生率、生存时间及外周血CD4＋和CD8＋T细胞比例的观测。

A series of P-T tests in field and model bin were carried out,through controlling initial pressure upper limit P and gastight degree,The results showed that gastightness not only relates to airtight degree,structure material and technological quality,but also relate to pressure upper limit,temperature and so on.

文章通过控制初始压力上限P和气密程度，进行了一系列P -T试验，对室内模拟仓和实仓进行测试，通过试验和对实验结果数据的处理分析，发现气密性不仅与环境密闭程度、结构材料及工艺质量有关，而且与测压上限及温度等环境条件有关；并且，在相同气密条件下，容器不同初始压力下的半衰期之间存在很好的线性相关性。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

AIM: To investigate the effects of goat placenta immunoregulating factor on the expression of costimulatory molecules lineaged T cells in BALB/c mice. METHODS: Animal model for immunodeficiency made from BALB/c mice with whole-body irradiation by 5 Gy 60Coγ-ray was applied for research.

目的：分析羊胎盘免疫调节因子对BALB/c小鼠T淋巴细胞共刺激表面抗原分子表达及其细胞因子分泌的影响，探讨羊胎盘免疫调节因子免疫促进作用机理。

Methods: The sample consisted143 children which were in early permanent dentition of ClassⅡ,Division 1malocclusion .Pre-and post-treatment study models were assessed using PeerAssessment Rating index. According to the outcome of treatment, thesecases were classified into three types as "worse, no different","improved"and"greatly improved". The cephalometric analysis and Model Analysis werepreformed with pretreatment lateral cephalograms and study models for"improved"and "greatly improved"cases."T"–test was assessed to find out thesignificant difference between the extraction and nonextraction, and the resultsincluding age factor were analyzed by stepwise discriminant analysis.

应用PAR 指数对矫治前后的143 副恒牙早期的安氏Ⅱ类1 分类错牙合患者牙牙合模型进行评估分析，按矫治结果等级分为三类A、变坏或无改善，B、改善，C、极大改善，然后将B、C 两类患者矫治前的头影测量片及模型分析数据按拔牙与未拔牙进行成组t 检验的单因素分析，找出两组间有显著差异的软硬组织和牙牙合模型指标，对这些指标合并年龄因素进行逐步判别分析。

Methods: Movement fatigue group serve as model, and one time exhaustion movement was given in each group, examined their blood glucose, oxypropionic acid, urea nitrogen, testosterone, corticosterone contents and myocardium superoxide dismutase activity and malonaldehyde contents and ocmputed T/C.

以运动性疲劳大鼠为模型，测定大鼠血糖、血乳酸、血尿素氮、血睾酮、皮质酮水平、心肌SOD活性、MDA含量及计算T/C比值。

On the basis of P-T estimated results and geophysical data, the petrological zoning model of Mesozoic lithospheric mantle in eastern North China block is set up in the thesis.

依据深源岩石包体的P-T条件估算结果，结合地球物理测深资料，本文建立了华北地块东部中生代岩石圈地幔的岩石学分带模型。

Mehods: After making the AD models by Aβ(subscript 1-42), draw the whole brain or hippocampus of rats in ordinary group, model group, traditional Chinese Medicine prevention group, traditional Chinese Medicine treatment group and positive control-group, and detect the biochemical marks of T-AOC, MDA, NO, NOS, chAT, TChE.

用凝聚态Aβ(下标 1-42)制备出AD模型后，取正常组、假损伤组、模型组、中药预防组、中药治疗组、阳性药物对照组各组大鼠的全脑或海马检测T-AOC、MDA、NO、NOS、chAT、TchE等指标。

RESULTS: The concentration-time curve of domperidone with untaking or taking famotidine was conformed to a two-compartment model. Under untaking and taking famotidine conditions, the main pharmacokinetic parameters, t, C and AUC(subscript 0-tn), were 0.63±0.36 and 1.50±0.97h, 9.91±5.45 and 4.30±5.01μgL^(-1), 39.57±10.46 and 32.43±9.61μghL^(-1) respectively.

结果：未服和服用法莫替丁后单剂量口服多潘立酮的血药浓度时间曲线均符合二室模型，达峰时间t分别为0.63±0.36、1.50±0.97h，峰浓度C分别为9.91±5.45、4.30±5.01μgL^(-1)，曲线下面积AUC(下标 0-tn)分别为39.57±10.46、32.43±9.61μghL^(-1)。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。