查询词典 model after

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

After two-cluster testing,branch length testing and relative rate testing ,two sequences(Melantitta nigra and Aythya americana) of 15 Cty b sequences were deleted because thek evolutionary rate were heterogeneous. The divergence between Anseriformes and Galliformes was chosen as an anchor point. Nucleotide sequence and amino acid sequence of 13 sequences data set was used to calibrated Anatidae molecular clocks. The results indicated that amino acid sequence is better than nucleotide sequence when divergence time is calculated with two kinds of sequences. The reliable divergence time of Anatidae were estimated with """"1+2TiTv"""" model.

经过二簇检验、分枝长度检验和相对速率检验，删除2条速率不恒定的序列后，用剩余的13条序列，以鸡形目与雁形目的分歧时间(89.8MYA)为锚定点，用顺序标定法标定鸭科鸟类分子钟的分析结果表明，用&1+2TiTv&分析得到的鸭科鸟类的分歧时间比较可靠；氨基酸序列用泊松校正模型和Γ模型估计的分类单元间的分歧时间则比较一致，表明氨基酸序列更适合用于鸭科鸟类分子钟的研究；用同一基因标定分子钟时，较长序列得到的结果标准误比较小；与核苷酸序列相比，氨基酸序列更适于用作分子钟的标定。

Changes of the blood gases and acid-base status of eleven healthy adult horses be-fore and after anesthetization kith Jing-Song-Ling,Chloral Hydrate and Laser were monitored with model DH-100G blood gas and acid-base analyzer.

以动脉血气分析做为手段，对静松灵、水合氯醛、激光三种麻醉方法进行评价，激光麻醉对实验马血气及酸碱值没有显著影响；静松灵（1mg／kg体重，im）和水合氯醛（128／100kg体重，iv）都能使PO＿2、Sat·O＿2、C－O＿2等显著下降，但水合氯醛出现较早，在注射后30至60min时最低；而静松灵出现较晚，以注射后60～90min显著，但都未构成低氧血症，更未达呼吸衰竭。

In vivo study, after murine angiosarcoma model establishment, seventy mice were divided into trial and control group with 5 mice for each group.

体内实验中建立小鼠血管肉瘤模型70只，各实验组与对照组均为5只，均静脉或腹腔给药。

Methods: Diabetic nephropathy rats induced by intraperitoneal injection of STZ were randomized into PN model group, n=10, Vitamin B6 group (group B, n=10) and aminoguanidine group (group A, n=10), contrasted to anormal control group (group C, n=10). Aminoguqnidine and VitB6 with 1 g dissolved in 1 L distilled water were given to group A and B, while group C and D only had 1 L disstided water. All groups drunk water freely after 24 hours.

利用链脲菌素腹腔注射法诱导建立DN大鼠模型，将其随机分为模型组、维生素B6治疗组、氨基胍治疗组，并与正常对照组比较，每组10只大鼠；A、B组每天分别用氨基胍、维生素B61g溶于1L蒸馏水中，C、D组仅给予1L蒸馏水，24h后自由饮用。

I do not focus on the Limits to Growth world model because I want to pick on its potent political implications (the first version did, after all, inspire a generation of antigrowth activists).

我之所以把焦点放在&增长的极限&世界模型上，不是因为我想选择它那些强有力的政治内涵（毕竟，它的第一个版本给予了一代反增长的激进主义分子灵感）。

I do not focus on the Limits to Growth world model because I want to pick on its potent political implications (the first version did, after all, inspire a generation of antigrowth activists).

我之所以把焦点放在&增长的极限&世界模型上，不是因为我想挑剔它的那些强有力的政治内涵（毕竟，它的第一个版本给予了一代反增长的激进主义分子灵感）。

I do not focus on the Limits to Growth world model because I want to pick on its potent political implications (the first version did, after all, inspire a generation of antigrowth activists).

我之所以把焦点放在&增长的极限&这个模型上，不是因为我想对它的那些强有力的政治内涵做什么挑剔（毕竟，它的第一个版本给予了整整一代反增长的社会活动积极分子以灵感）。

After adopting proper appraisable indexes and confirming indexes weight, synthetical appraising value was computed, the digital environmental model of the risk assessment of debris flow and the risk grade diagram of criticality assessment of debris flow range in Dazhaigou valley are established.

再通过合理选取评价指标、建立指标权重体系、计算综合评价值等一系列程序，建立了泥石流发生域危险性评价的数字环境模型，绘制了大寨沟流域泥石流发生域危险性等级评价图。

Refer to the domestic and foreign literature material, to establish traumatic joint stiffness model with New Zealand big white rabbit;caused the right knee joints of the big white rabbits closed trauma with the appropriate mothod,then fixed the behind-right knee joints 4 weeks with the gypsum and the bandage;after 4 weeks, removed fixture,examined the animal blood rheology,the joint range,the skin temperature, the arthrocele extent and so on ,to authenticate whether modeling succeeded or not.

参考国内外文献资料，用新西兰大白兔建立创伤性关节僵直模型；用适当的方法造成大白兔右后膝关节闭合性损伤，用石膏绷带、绷带固定4周，4周后拆除固定。期间观察皮肤温度、关节肿胀度、关节活动度，检测动物血液流变学等指标，以验证模型建立是否成功。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。