查询词典 model after

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Objective To use an ischemical reperfusion injury rat model by pulsinelli-4-VO andobserve the changes of the expression of Glu, N-methy-D-asparagic acidreceptor subunits NR_1 and NR_(2B) and ability of learning and memory after procerebrum ischemic reperfusion injury, and approach the pathogenesy of learning andmemory disorder induced by ischemic reperfusion injury, and the intervention ofginsenoside Rg_2 on the learning and memory disorder after procerebrumischemic reperfusion injury.

目的利用pulsinelli四血管法体建立脑缺血／再灌注损伤模型，观察前脑缺血损伤后谷氨酸、N-甲基-D-天门冬氨酸受体亚单位NR_1、NR_(2B)表达的变化及学习记忆的改变，从兴奋性氨基酸的兴奋毒作用尤其是其受体的表达方面探讨脑缺血／再灌注损伤引起的学习记忆功能障碍的发生机制，并观察人参皂苷Rg_2对脑缺血缺氧损伤引起的学习记忆障碍的干预机制。

The model of brain concussion of rat can be established successfully using iron pendulum hitting device; 2. The spatial learning and memory deficits of the rats are detected by MWM in early period post-BC(from 1 to 3 days after BC); 3. Pycnosis degeneration or necrosis neurons of the cerebral cortex, dorsal hippocampus, dentate fornix and brainstem reticular formation are identified in BC rats; 4. There are significantly changes in the number and activity of cholinergic neurons in the basal forebrain and brainstem reticular formation after BC; 5. The change of cerebral neurons and cholinergic neurons correlate with cognitive deficits in BC rats .

1、单摆式闭合性机械打击装置能成功建立脑震荡大鼠模型；2、脑震荡大鼠在MWM实验中出现早期空间认知行为障碍；3、脑震荡大鼠大脑皮质、背侧海马、齿状回和脑干网状结构出现程度不等的部分神经元固缩变性和不完全性坏死；4、脑震荡大鼠基底前脑、脑干网状结构ChAT活性表达有明显变化；5、脑震荡大鼠认知障碍与相关脑区神经元、胆碱能神经元的变化有关联。

Methods: A brain concussion was produced in rats using a simple pendulum device model. One control (n=12) and six experimental groups were used(n=12 in each group). The cerebral concussion groups were assigned to sacrifice times ranging from 1 day to 24days after injury.A Morris Water Maze memory paradigm was used to assess learning and memory function. Pycnosis degeneration or necrosis neurons in the locus cerebral cortex, dorsal hippocampus, dentate fornix and brainstem reticular formation were observed and counted after brain concussion. Cholinergic neurons in the basal forebrain and brainstem reticular formation were identified and quantitated using choline acetyltransferase.

应用自制单摆式闭合性机械打击装置建立脑震荡大鼠模型；成年SD大鼠随机分为1个对照组(n=12)和6个脑震荡组(即伤后1、2、4、8、16、24d，n=12)；应用Morris水迷宫(Morris Water Maze，MWM)实验评价大鼠的学习记忆功能；用光镜对大鼠的上述脑区的固缩变性坏死细胞进行观察和计数；用免疫组化方法检测基底前脑和脑干网状结构的胆碱乙酰转移酶(cholin acetyltransferase，ChAT)阳性细胞的表达。

Taking household\'s land use preference as classification rule, household agent types were divided though classification tree. Different choosing process of current production strategies and theirs probabilities after expiration of GFGP chosen by various types of household agents\' were put forward by experience-weighted attraction model based on bounded -rational approach. Finally, forecasting the results of households\' recultivation behaviour after the expiration of GFGP by scenario analysis method. The results show that relative policies are useful to rectify deviation of households\' bounded-rational behaviours and reinforce outcomes of GFGP.

以农户家庭户主的土地利用选择偏好作为分类依据，采用分类树方法对农户智能体类型进行划分；基于有限理性决策理论，引用经历—权重吸引模型，对农户智能体的目前生产策略决策过程和退耕还林政策期满后农户对不同生产策略的选择概率进行了分析；最后，在ERDAS环境下对退耕还林政策期满后农户的复垦行为进行了情景分析，认为政府制定并实施的相关政策措施对于纠正农户的有限理性行为偏差，维持和巩固退耕还林政策成果具有重要作用。

After analyzing the character of sinkable chemicals and that of all action progress after this type of chemicals spilled into the sea, the paper established corresponding numerical model and programmed them.

根据国际和国内在有关公约和法规方面的有关规定，在研究了化学品海上运输对环境造成危害的基础上，从管理的角度出发分析建立相应的污染事故应急反应体系的迫切性。

Methods Thirty-six Wistar rats were randomly divided into four groups: group Ⅰ received intraperitoneal injection of L-thyroxine for 42 days to establish a model of thyrotoxic myopathy; group Ⅱ was treated with XYQJT for 30 days after L-thyroxine intraperitoneal injection; group Ⅲ was given normal saline for 30 days after L-thyroxine intraperitoneal injection as control; group IV served as normal control group.

方法36只健康雌性Wistar大鼠随机分为4组：甲亢肌病模型组10只，腹腔注射甲状腺激素钠盐每天1次，共42d；中药治疗组10只，腹腔注射甲状腺激素钠盐42d后，灌服消瘿强肌汤30d；生理盐水对照组10只，腹腔注射甲状腺激素钠盐42d后，灌服生理盐水30d。

Methods:Using the model to construct PTSD-like rats. SPS is not performed in the control group and the rest three groups are undisturbedly raised for respectively 24h, 7d and 14d after SPS stimulation. PTSD-like symptoms can be seen in the rats group 7 days after SPS which is the PTSD-like group.

使用SPS方法建立PTSD样大鼠模型，无SPS刺激组为对照组，其余三组分别为SPS刺激后静养24h、7d和14d，其中7d组出现PTSD样症状为PTSD样大鼠组。

However, different from Salant model, I do not restrict all firms to remain the Cournot strategies before and after the merger, but allow the new merged group a Stackelberg leader and the unmerged firms remain Cournot after the merger.

但是，与Salant模型不同的是，本文不再假定企业在兼并前后始终保持古诺竞争策略，而是假定当兼并发生之后新企业采取的是斯塔伯格竞争策略，而未参与兼并的企业仍然采用古诺竞争策略。

Our researches include:To establish the rat experimental model of VILI and study the the activation of the receptor of cellular membrane in VILI;To observe the change of transmembrane signal transduction and inflammation cytokine after applying the specific blocking agent of receptors;To observe the change of transmembrane signal transduction and inflammtion cytoking after applying the no specific blocking agent of receptors.

主要内容建立大鼠VILI实验模型，观察各种细胞膜受体（包括整合素受体、生长因子受体、G蛋白偶联受体）及其配体在大潮气量机械通气时的表达变化；应用各种受体的特异性阻断剂，观察对大鼠VILI信号转导通路以及炎症因子表达的影响；应用各种非特异性阻断剂，观察对大鼠VILI信号转导通路以及炎症因子表达的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。