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The reaction was run in aqueous diethyl ether at 30℃ for 24h under the catalysis of bakers' yeast, which preincubated for 2h in the presence of phenacyl chloride.

实验结果表明，乙醚为适宜反应介质，适宜pH为中性；反应初始水含量和初始底物浓度分别以w(H2O)=30g/L、c=5 mmol/L为佳。

Results The strongest photoinactivation effect occured in the HEP-2 cells co-cultured with 1 mmol/L ALA for 8 h. The killing activity of ALA-PDT to the HEP-2 cells was positively correlated with the power density of the irradiation (r=0.88, P＜0.05).Conclusions ALA-PDT can effectively kill the cultured HEP-2 cells in vitro. The optimal killing activity was related to the time and the power density of the laser irradiation.

结果 (1)光照时机选择在ALA与HEP-2细胞培养8 h时杀伤效应最强；(2)ALA-PDT对HEP-2细胞的杀伤效应与照光能量呈正相关r=0.88，P 结论 ALA-PDT能有效地杀伤体外培养的肿瘤细胞，其杀伤最佳效果与照光时机和照光能量有关。

The contents of chlorophyll were also decreased. New protein spots were induced and some protein spots were lost in root, plumular axis, chloroplast, when seedlings were treated with 1.0 mmol/L Cd(superscript 2+). A positive relationship was observed between the inhibition of seedling growth and changes in biochemical characteristics.

在1.0mmol/L Cd(上标 2+)溶液处理下，与对照相比，黄瓜幼苗的根、胚轴、叶绿体中的蛋白质组分发生变化，即蛋白缺失、新蛋白合成以及蛋白表达量的上调或下降，同时其生化性状（蛋白质组分、叶绿素含量）的变化与幼苗生长抑制呈正相关。

Ca 2+ at 1 64 mmol/L markedly increased ethanol tolerance of a self_flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae. After 9 h of exposure to 20% ethanol at 30℃, no viability remained for the control whereas 50 0% remained for the cells both grown and incubated with ethanol in Ca 2+_added medium.

生长阶段和冲击阶段均添加 1 6 4mmol LCa2 +能显著提高自絮凝颗粒酵母于 30℃在 2 0 %酒精冲击下的存活率，经过 9h冲击，对照组的存活率为 0 ，而添加Ca2 +试验组的存活率为 5 0 0 %，表明添加适当浓度的Ca2 +能显著提高菌体的耐酒精能力。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后，分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h，研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

03 And 5 mmol/L on cellular ultrastructure,Ca localization and elemental subcellular distributions in Pteris vittata L.

本文对砷和钙处理下蜈蚣草羽叶中砷、钙的亚细胞分布、超微结构变化及钙定位进行了研究。

The DNA of Yak PrP expressed plasmid extracted from the recombinant expressed bacterium was identified by PCR amplification and two-ezyme digestion. The expressed product was obtained from the recombinant bacterium inducted under the optimized expressed condition (37℃, 1 mmol/L IPTG, 6 h), and it was determined by SDS-PAGE and Western blotting. The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies; the second is to separate by SDS-PAGE.

将保存的含有牦牛PrP基因重组表达菌提取质粒DNA，进行PCR扩增和双酶切鉴定，并在优化诱导表达条件(37℃，1mmol/L IPTG，6h)下，获得的表达产物进行SDS-PAGE和Western blotting检测；将鉴定之后的重组表达菌诱导表达后，通过两种方法回收GST-BoPrP(23～242)融合蛋白：其一，是从包涵体中提取和复性GST-BoPrP(23～242)融合蛋白；其二，是通过SDS-PAGE直接分离并纯化GST-BoPrP(23～242)融合蛋白。

ResultsLoureirin B inhibits the TTXR peak sodium currents in a dose-dependent model and the inhibition is reversible. 0.2mmol/L loureirin B accelerates the inactive process of the TTXR sodium currents and delays the revivable process but does not affect the activitive process.

结果龙血素B对TTXR钠电流呈浓度依赖性抑制，且抑制作用可逆；高浓度的龙血素B（0.2 mmol/L）加速TTX-R钠通道电流的失活过程，延迟其复活过程，对激活过程无显著影响。

ResultsLoureirin B inhibits the TTXR peak sodium currents in a dose-dependent model and the inhibition is reversible. 0.2mmol/L loureirin B accelerates the inactive process of the TTXR sodium currents and delays the revivable process but does not affect the activitive process.

结果龙血素B对TTXR钠电流呈浓度依靠性抑制，且抑制作用可逆；高浓度的龙血素B（0.2 mmol/L）加速TTX-R钠通道电流的失活过程，延迟其复活过程，对激活过程无显著影响。

It was suggested that moderate fatigue induced inhibition of myofibrils and sarcoplasmic reticulum Ca2+ release channels.

在S50P与L50P间断强直收缩模式下，疲劳期结束时，30 s内用5 mmol/L咖啡因(肌浆网Ca2+释放通道选择性开放剂)灌流，使肌条恢复20 min。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。