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Specificity studies on the substrate revealed that the HdPO was capable of oxidizing not only the o-diphenol, but also tyrosine. The apparent Km value on tyrosine was about 9.52 mmol/L. As shown in the experiments, HdPO behaved oxidizing activities both on diphenol and monophenol, which implies that the HdPO was probably not only a type of tyrosinase-type PO but also a catechol-type.

底物特异性研究还发现此HdPO不仅对邻苯二酚具有氧化活性,对酪氨酸底物也具有氧化活性,且对酪氨酸的Km值约为9.52mmol/L,说明该酶既具有酪氨酸酶活性,又具有儿茶酚酶活性。

And dissimilatory reduction capability of crystal Ferric phosphate is also investigated when using different organic acid as substrates. Water-extracted soil solutions of SiChuan, JiangXi, JiLin and HuNan were incubated under anoxic atmosphere.

在水稻土浸提液为接种液的富集培养中添加不同浓度的磷酸缓冲液作为磷源,当磷酸盐浓度大于30 mmol/L时,铁还原反应的速率和Fe的产生量相对较低,甚至有抑制作用。

In a word, the treatment of 50,100 mmol/L NaCl and Na2SO4 enhanced Elaeagnus angustifolia L.

并且影响其发芽率盐浓度存在一个阈值(300mmol/L),含盐量低于该阈值宜于其发芽和生长。

In Gabon , chimpanzees had a biscuit diet of constant nutrient composition except that the sodium content was changed episodically over 3 years from 75 to 35 to 120 mmol/d.

作者对来自加蓬的17只猩猩和美国德克萨斯州的110只猩猩进行钠盐摄入改变的观察试验。其中,加蓬的17只猩猩在3年的观察时间内分段式给予每天75,35,120mmol 的钠盐。

The passage 3 rat bone marrow mesenchymal stem cells were cultured at the density of 5×107/L in different groups, the induced group was added into conditioned medium containing 1×10-8 mol/L dexamethasone, 10 mmol/L beta-sodium glycerophosphate and 50 mg/L ascorbic acid at the same time of seeding, at 50%-60% confluence, at 85%-90% confluence, respectively.

将第3代大鼠骨髓间充质干细胞以5×107 L-1密度分组培养,诱导组分别于接种当时、细胞达50%~60%融合时、细胞达85%~95%融合时加入含地塞米松、β-甘油磷酸钠、维生素C的成骨诱导培养基诱导。

Low concentration of NaCl solution could promote the growth on the shoot of seed germination. The growing length of roots and shoots of two halophytes treated by 100 mmol·L~(-1) NaCl reached the highest and the growing condition on shoots was best. Then some physiological and biochemical parameters including the content of Na~+, K~+, Ca~(2+), proline and plant leaf tissue water potential, the assays of membrane lipid peroxidation and antioxidant enzymes were determined with the two halophytic plants on salinity.

研究工作首先对盐爪爪和盐穗木在种子萌发阶段的耐盐性进行了比较研究,表明盐爪爪和盐穗木都是很耐盐的盐生植物,在种子萌发期,盐穗木比盐爪爪更耐盐;NaCl和等渗的聚乙二醇对种子的萌发都产生抑制作用,且PEG的抑制程度大于等渗的NaCl。

In the absence of organic solvent and phase-transfer reagent, the catalytic oxidation of cyclo- hexene to adipic acid with aqueous 30% hydrogen peroxide can be achieved in the presence of tungstic acid/acidic organic additive.

以钨酸/有机酸性添加剂为催化体系,在无有机溶剂、相转移剂的情况下,催化 30%过氧化氢氧化环己烯合成己二酸。当钨酸∶有机酸性添加剂∶环己烯∶过氧化氢=1∶1∶40∶176(摩尔比,钨酸用量为 2.5 mmol)时,使用有机酸性添加剂考察钨酸的催化性能,结果表明以钨酸/间苯二酚催化氧化环己烯的催化效果最优,反应 8 h 时己二酸分离产率达 90.9%、纯度为~100%;而不使用有机酸性添加剂时,己二酸分离产率只有 72.1%,产品纯度为 96.2%。

Results: Within-run CV 、day-to-day CV and total CV were 1.33%、1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6%; anticoag-ulant、septic、uric acid and bilirubin under a certain concentration also did not interfere with the glucose re-sult in this method. Compared with HK method, the pertinence was Y =1.038X-1.172,r =0.994. The linearity studies showed the method was linear up to 20mmol/L.

结果: 重复性试验批内 CV 值为1.33%,批间CV 值为1.42%,总 CV 值为2.01%;回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%,93.5%和91.4%,平均为96.6%;干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰;比较试验,与HK法比较其相关性为Y =1.038X-1.172,r =0.994;线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。

Results: Within-run CV 、day-to-day CV and total CV were 1.33%、1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6%; anticoag-ulant、septic、uric acid and bilirubin under a certain concentration also did not interfere with the glucose re-sult in this method.

结果: 重复性试验批内 CV 值为1.33%,批间CV 值为1.42%,总 CV 值为2.01%;回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%,93.5%和91.4%,平均为96.6%;干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰;比较试验,与HK法比较其相关性为Y =1.038X-1.172,r =0.994;线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。

The molecular weight of dextransucrase measured by SDS-PAGE was 170 kDa ,which was similar to the enzyme from Leuconostoc mesenteroides. The enzyme had an optimum temperature between 25and 30℃ and an optimum pH of 5.4. It was relatively stable in the range of pH 5.0 to 7.0, but the stability declined rapidly as soon as the temperature rose over 35℃.

以蔗糖为底物,酶促反应的最适温度为25~30℃,最适pH值为5.4,动力学常数Km值为10.43 mmol/L;酶活在pH 5.0~8.0较为稳定,在室温(25℃)保藏4 天仍有59%的酶活力,4℃保存7周酶活力仅下降一半,但在35℃以上失活很快;Ca2+对催化作用有较大的促进,Mg2+有微弱的促进作用,K+对催化反应无影响,Cu2+的抑制作用最强。

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