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RESULTS The average of total cholesterol,famish blood glucose,triglyceridein male were 4.62 mmol/L,4.37 mmol/L,1.68 mmol/L,respectively.

结果男性胆固醇、血糖、甘油三酯均值分别为4.62mmol/L、4.37mmol/L和1.68mmol/L，女性胆固醇、血糖、甘油三酯均值分别为4.99mmol/L、4.57mmol/L和1.61mmol/L。

The best formula of different protectors optimized by orthogonal experiment design consist of trehalose 1.75 mmol/L,mannitol 1.5 mmol/L and raffinose 2.0 mmol/L.

采用正交实验设计优化出最佳保护剂配方，即1.75mmol／L的海藻糖，1.5mmol／L的甘露醇和2mmol／L的棉籽糖。

6 Buffer comprised 6 mmol/L sodium phosphate, 10 mmol/L sodium borate, 25 mmol/L sodium dodecyl sulphate is used as runing solution, and UV detection wavelength is at 227 nm to determining disperse blue 2BLN in dyeing wastewater.

6mmol/L磷酸钠、10mmol/L硼砂、25mmol/L十二烷基磺酸钠组成的pH9 6的缓冲溶液作电泳液，UV检测波长227nm，测定了印染废水中分散蓝2BLN 的含量。

The electrolyte buffer for the anion separation consisted of 40 mmol/L boric acid -1.8 mmol/L potassium dichromate -2 mmol/L sodium tetraborate (pH 8.6), and tetramethyl ammonium hydroxide was used as electro-osmotic flow modifier.

阴离子测定采用的缓冲体系为40mmol/L硼酸-1.8mmol/L重铬酸钾-2mmol/L硼酸钠(pH8.6)，氢氧化四甲铵为电渗流改性剂，5种阴离子在4.6min内达到基线分离，检出限为0.10~1.85mg/L。

The whole analysis was completed on a C〓 column in 18min at a flow rate of 0.5 ml/min, with the mobile phase of methanol-water (93: 7, v: v) containing 75 mmol/L TEOLA and 200 mmol/L HAc, or with the mobile phase of ethanol-water (90: 10, v: v) containing 25 mmol/L TEOLA and 200mmol/L HAc pumped at 0.25ml/min. Compared with the methanol system, the ethanol system was a better candidate for the separation of thulium porphyrin complexes in view of selectivity.

在C〓分析柱（5μm，2.5×150mm）上，流动相为：甲醇：水＝93：7（v/v内含75mmol/L三乙醇胺和200mmol/LHAc），流速为0.5ml/min和流动相为：乙醇：水＝90：10（v/v，内含25mmol/L三乙醇胺和200mmol/L HAc），流速为0.25ml/min，均能使六个铥卟啉配合物在18min内得到有效的分离，相比之下，乙醇体系对铥卟啉配合物更具有分离选择性。

Results The reaction was not turbidness which was between modified reagent containing potassium hydroxide 143 mmol/L, tartrate kaliumna natrium 56.7 mmol/L and blood serum containing triglyceride 5.6 mmol/L,glucose polymer 60 g/L and need not sample blank.

结果含氢氧化钾 143mmol/L、酒石酸钾钠 56.7mmol/L的改良试剂与含 TG 5.6mmol/L、葡聚糖60g/L的血清反应均不显混浊，可不设标本空白。

objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响，联苯胺染色法检测血红蛋白合成情况，应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04～0.09？mmol／l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002～0.01？mmol／l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成，2种单宁化合物(0.01～0.05？mmol／l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成，特里马素i(0.01？mmol／l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

A baseline separation of Sitafloxacin epimers was achieved with a background electrolyte of 10 mmol/L KH2PO4-K2HPO4 (pH 4.5), 10 mmol/L CuSO4, 20 mmol/L γ-CD and 10 mmol/L D-Phe. The linear range for Sitafloxacin was 32-400 mg/L (0.996). The relative standard deviations of migration time and peak area were less than 1.9% and 3.8% respectively.

实验结果表明，添加剂的种类和浓度是影响西他沙星手性分离的重要因素，只有当D-苯丙氨酸、铜离子和γ-环糊精同时存在并达到一定浓度时，西他沙星差向异构体在毛细管电泳中才具有良好的分离效果。

In this experiment, 250mL Erlenmeyer flask with 50mL reaction liquid(400mmol/L glucose, 10mmol/L CMP, 50mmol/L choline phosphate, 100mmol/L K 2HPO 4 KH 2PO 4, 10mmol/L MgSO 4, pH=8.0) and 1 200g/L immobilized cells were used. After 4h incubation 400mmol/L of glucose was supplemented.

结果表明：2 5 0 m L摇瓶中加入 5 0 m L反应液(葡萄糖 40 0 mmol/ L，CMP1 0 mmol/ L，磷酸胆碱 5 0 mmol/ L，K2 HPO4 -KH2 PO4 缓冲液 1 0 0 mmol/ L，Mg SO4 1 0 mmol/ L，p H=8.0 )，固定化细胞 1 2 0 0 g/ L，3 4°C、1 5 0 r/ min下，反应 4h后补加 40 0 mmol/ L葡萄糖，反应 8h可使60 %以上的 CMP转化为 CDP-胆碱。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。