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After optimizing the expression conditions, a higher production could be obtained when GS115-proCPB was induced in BMGY(pH 6.0), at 28℃, with addition of 0.5% casein acid, 3 mmol/L PMSF, and 0.5% methanol per 12 h. The yield of recombinant protein reached 500 mg/L, and achieved over 94% of total protein in the culture supernatant.

通过表达条件的优化，使用BMGY(pH 6.0)培养基，添加0.5%的酪蛋白水解物和3 mmol/L的PMSF，于28 ℃培养，每隔12 h补加0.5%的甲醇，重组酵母GS115-proCPB 表达的重组蛋白可达500 mg/L 以上，表达的目的蛋白占总蛋白的94%以上。

The dyes were extracted from textiles composed of natural or chemical fibers by methanol under ultrasounication, and then eluted with gradient by acetonitrile and 5 mmol/L ammonium acetate from an RP-C18 column with two segments in effluents. The first effluents accommodated Acid Red 26, Direct Blue 6, Direct Black 38 and Direct Red 28 with negative ionization mode, while the second accommodated Basic Red 9, Basic Violet 14, Disperse Blue 1, Disperse Orange 11 and Disperse Yellow 3 with positive ionization mode. Thus the investigated compounds could be identified simultaneously with single-run analysis no matter which type of the fibre the sample was and no matter which category of the dye the analyte was.

用甲醇超声同时提取天然纤维和化学纤维上的染料，以5 mmol/L乙酸铵和乙腈为流动相在C18柱上于前段洗脱酸性红26、直接蓝6、直接黑38和直接红28（采用电喷雾质谱负离子模式检测），于后段洗脱碱性红9、碱性紫14、分散蓝1、分散橙11和分散黄3（采用电喷雾质谱正离子模式检测），实现了对不同种类纤维织品中分属4类性质不同染料的一次提取和一次分析检测。

METHODS: The bile was treated with 3 different methods, including the 2D-Clean-up kit group: the bile sample was dissolved with lysis buffer (contains: 7 mol/L carbamide, 2 mol/L thiourea, ammonium persulfate with mass concentration of 0.4 g/L, 65 mmol/L dithiothreitol, carrier ampholytes with mass concentration of 0.2 g/L).

将标本分别按3种不同方法进行处理：①2D-Clean-up试剂盒处理胆汁标本：用裂解液，7 mol/L尿素，2 mol/L硫脲，质量浓度为0.4 g/L的过硫酸铵，65 mmol/L二硫苏糖醇，质量浓度为0.2 g/L的载体两性电解质溶解沉淀过夜。

The results showed that activity and thermostability of β-amylases were greatly improved by addition of 30 mmol/L Ca(superscript 2+) to soluble starch solution, whereas the formation of reducing sugar was slightly increased by the addition of Ca(superscript 2+) during liquidification.

研究表明，向可溶性淀粉溶液中添加30 mmol/L Ca(上标 2+)，能大大提高β-淀粉酶的活性和稳定性；而液化过程中的外加Ca(上标 2+)盐可使还原性糖的生成量略有提高。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

We believe that diuretics should be stopped once serum sodium is 125 mmol/l and the patient observed.

该领域所有的专家推荐如果血钠≤120 mmol/L，停用利尿剂。

The results showed that the spatial distribution range of soil fertility in Qinling forest area on south slope was soil pH volue 5.97～6.89,organic matter 6.6～50.1 g·kg~-1, total N 0.5～3.8 g·kg~-1 ,available N 33.6～257.3 mg·kg~-1,available P 0.81～5.8 mg·kg~-1,available K 38.9～262.4 mg·kg~-1,clay particle content 11.3%～18.1%,capacity of exchangeable base 68.5～310.2 mmol·kg~-1 respectively; The spatial variation degree of available P, organic matter, and total N was big,whereas clay particle content and active acid pH value was small;the spatial variation degree of soil pH value, organic matter, available P, clay particle content, and capacity of exchangeable base decreased with the deep of soil,whereas soil total N、available N, and available K increased.

结果表明，秦岭南坡土壤肥力指标在整个土壤剖面空间分异范围分别为：土壤pH值5.97～6.89，有机质含量6.6～50.1 g·kg-1，土壤全N含量0.5～3.8 g·kg-1，碱解N含量33.6～257.3 mg·kg-1，速效P含量0.81～5.8 mg·kg-1，速效K含量38.9～262.4 mg·kg-1，粘粒含量11.3%～18.1%，阳离子代换量68.5～310.2 mmol·kg-1；其空间分异程度以速效P、有机质和土壤全N含量较大，土壤粘粒含量和土壤pH值较小；随着土层的加深，土壤pH值、有机质、速效P、土壤粘粒含量和阳离子代换量空间分异程度减小，而土壤全N、碱解N和速效K含量空间分异程度增大。

The five-leaf seedlings were treated in nutrient solution containing 0.08 mmol/L HgCl2 for 8 d.

用0.08 mmol/LHgC l2溶液对水稻种子浸种24 h、5叶期幼苗根部胁迫处理8 d。

In this essay ,by mean of culturing with nutritional solution of different salt concentration (NaCl, mmol/L), and investigated the salt tolerance of four halophytes in two aspects of microstructure and physiological indexes.

本文采用不同盐浓度（NaCl， mmol/L）营养液培养的方法，从形态显微结构和生理生化指标两方面对四种植物的抗盐性进行了研究。

Methods The gradient elution separation of RPC18 column was made by 5 mmol/L heptafluorobutyric acid and methanol, respectively.

色谱条件采用RPC18色谱柱，流动相为5 mmol/L七氟丁酸和甲醇，梯度洗脱。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。