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Methods Brain microvascular endothelial cells and neuroglial cells of newborn rats were cultured in vitro, and treated with 1,2-dichloroethane at a dose of 0 μl/ml, 5 μl/ml, 10 μl/ml or 20 μl/ml.

方法］在体外建立了血脑屏障细胞模型，并从形态学上观察1，2-二氯乙烷对血脑屏障细胞的损伤作用。

Methods Eighty healthy nulliparous parturients scheduled for patientcontrolled epidural analgesia in labour, were randomly divided into 4 groups(n=20) according to different infusion speed:group I with a speed of 4 ml/h, group II with 6 ml/h, group III with 8 ml/h, group IV with 10 ml/h.

对80 例行自然分娩的单胎足月初产妇，均采用硬膜外自控分娩镇痛，根据不同背景输注速率随机分为四组，A组输注速率为4 ml/h，B组为6 ml/h，C组为8 ml/h，D组为10 ml/h。

The result showed that different concentration of FSH and LH were needed for sheep oocytes IVM in different season Xinjiang. FSH 12.9ug/ml,LH0.86IU/ml is proper to sheep oocytes IVM in summer-autumn anestrous period. FSH 8.6ug/ml,LH0.57IU/ml is proper to sheep oocytes IVM in autumn oestrous period.

结果表明：新疆绵羊卵母细胞在不同的季节中，体外成熟所需的FSH、LH浓度不同，夏秋乏情季节适宜于体外成熟的激素浓度为FSH 12.9ug/ml，LH0.86IU/ml；秋季发情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml，LH0.57IU/ml；春季乏情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml，LH0.86IU/ml。

The minimal bactericidal concentration of PTKE on Escherichia coli, Staphylococcus aureus, Salmonella, Pasteurella multocida and Streptococcus agalactiae were 100, 25, 50, 25 and 50 mg/mL respectively. The isolation of EA, EB and EC had no antibacterial effect on Escherichia coli and Pasteurella multocida. MBC of EA on Staphylococcus aureus and Salmonella were 2.5 and 5 mg/mL, and MBC of EB on Staphylococcus aureus, Salmonella and Streptococcus agalactiae were 1.25, 5 and 2.5 mg/mL respectively, while MBC of EC on Staphylococcus aureus was 2.5 mg/mL. Polygonum taibaishanense Kung.

PTKE对大肠埃希菌、金黄色葡萄球菌、沙门氏菌、多杀性巴氏杆菌和无乳链球菌的最低杀菌浓度分别为100，25，50，25和50 mg/mL；而PTKE的分离物EA、EB和EC对大肠埃希菌和巴氏杆菌无抑菌作用，EA对金黄色葡萄球菌、沙门氏菌的MBC分别为2.5和5.0 mg/mL，EB对金黄色葡萄球菌、沙门氏菌和无乳链球菌的MBC分别为1.25，5.0和2.5 mg/mL，EC对金黄色葡萄球菌的MBC为2.5 mg/mL。

The results indicate that the presence of GSH increased the expression of exoY、exoS、phzA1 and gacA,while no difference was observed with others virulence factor genes tested.PAO1 produced markedly less pyocyanin than the wild type strain PAOIP＜0.01Treating with GSH, the PCN production of PAOI(△gshB was increased from 0.47μg/mL to 1.73μg/mL (P＜0.01),the PCN production of PAO1 increased from 1.3μg/mL to 1.9μg/mL(P＜0.05),this result indicates that GSH upregulates PCN production.

结果发现，GSH对启动子库中exoS、exoY、phzA1和gacA等致病性因子的表达有促进作用，对其余测试过的致病性因子则没有明显影响；铜绿假单胞菌分泌的重要致病因子绿脓菌素(pyocyanin，PCN)的含量，在PAO1中远低于PAO1P＜0.01加入GSH后，PCN平均含量在PAO1(△gshB中从0.47μg/mL提高到1.73μg/mL，差异十分显著(P＜0.01)，在PAO1中从1.3μg/mL提高到1.9μg/mL，差异显著(P＜0.05)，表明GSH对铜绿假单胞菌生产PCN有促进作用。

Using New Zealand rabbit ears cartilage makes chondrocyte suspension, Fibrinogen was mixed with D-hank's makes a final fibrinogen concentration of 50mg/ml mixture solution, then chondrocytes resuspend with fibrinogen solution, chondrocytes-seeded fibrinogen was mixed with thrombin (25U/ml in 40mM calcium choride) to make chondrocytes/fibrin glue polymer. Pluronic F-127 was mixed with D-hank's makes a final Pluronic F-127 concentration of 400mg/ml mixture solution, then chondrocytes resuspend with Pluronic F-127 solution to make chondrocytes/Pluronic F-127 polymer. The chondrocyte concentrations was 10 million chondrocyte/ml of polymer.

为确定纤维蛋白凝胶与Pluronic F-127在注射方式形成组织工程化软骨过程中的优劣，我们进行了如下实验：应用新西兰大白兔耳软骨获取软骨细胞并体外培养，纤维蛋白原应用D-hank's液配制为50mg/ml，然后以纤维蛋白原溶液重悬软骨细胞，再与40mM的氯化钙配制的25U/ml凝血酶混合形成软骨细胞—纤维蛋白凝胶复合物；Pluronic F-127应用D-hank's液配制为400mg/ml，同样用PluronicF-127溶液重悬软骨细胞而形成软骨细胞—Pluronic F-127凝胶复合物。

Among the 45 neurons generating phasic firing, 8 (17.78﹪) neurons could still be induced phasic firing after treatment with 1×10-2 μg/mL SVHRP and 37 (82.22﹪) neurons had no responses to the stimulation. The AP firing of neurons was dramatically different after treatment with SVHRP (P＜0.01, n=45). Among the 7 repetitive firing neurons, all of them could only generate 1 or 0 AP instead of repetitive firing when SVHRP was applied. The number of APs was 14.57±1.00 and 0.57±0.20 before and after SVHRP treatment (P＜0.01, n=7). The AP rheobase was (75.10±8.99) pA and (119.85± 12.73) pA before and after 1 × 10-4 μg/mL SVHRP application, respectively (P＜0.01,n=8). The AP threshold was increased from (-41.17±2.15) mV to (-32.40±1.48) mV after 1×10-4 μg/mL SVHRP treatment (P＜0.01,n=8). The peak amplitude of AP was (68.49±2.33) mV for the neurons before treatment with 1×10-4 μg/mL SVHRP and (54.71±0.81)mV after treatment (P＜0.01, n=8). These results showed that SVHRP could decrease the AP firing frequency, increase the AP rheobase and threshold, but decrease the AP peak amplitude of hippocampal neurons.

在产生位相放电的45个细胞中，有8个细胞在SVHRP处理后仍可以诱发出位相放电(占17.78﹪)；37个细胞在SVHRP处理后无法诱导出位相放电(占82.22﹪)，SVHRP处理后动作电位的产生与处理前相比，有显著差异(P＜0.01，n=45)；在产生重复放电的7个细胞中，在1×10-2μg/mL SVHRP作用后均不能再次诱发出重复放电，而是产生一个动作电位或不再产生动作电位，药物处理前产生的动作电位个数为14.57±1.00，SVHRP处理后产生动作电位的个数为0.57±0.20，二者之间有显著性差异(P＜0.01，n=7)。1×10-4 μg/mLSVHRP处理后，诱发动作电位产生的基强度由(75.10±8.99)pA增加到(119.85±12.73)pA(P＜0.01，n=8)；阈电位由(-41.17±2.15)mV升至(-32.40±1.48)mV(P＜0.01，n=8)；动作电位峰值由(68.49±2.33)mV下降至(54.71±0.81)mV(P＜0.01，n=8)。

We use the EGF 0.1ng/ml,1ng/ml,10ng/ml;100ng/ml to stimulate BT325cells separatively and find that with increased dosage,the growth andproliferation of BT325 was apparently inhibited.

分别给予每毫升0.1ng，1ng，10ng，100ng EGF刺激BT325细胞，发现并证实随着剂量的增大，BT325细胞生长增殖明显的抑制。

Results The concentration of Chlorogenic acid was 0.05mg/ml, 0.1mg/ml, 0.4mg/ml, 0.8mg/ml respectively, it can respectively inhibit respiratory syncytia virus, Coxsackie B3 virus, adeno-associated 7 virus, adeno-associated 3 virus and Coxsackie B5 virus.

结果 氯原酸浓度分别为0.05mg/ml、0.1mg/ml、0.4mg/ml、0.8mg/ml时，可分别体外抑制合胞病毒、柯萨奇B3、腺病毒7型、腺病毒3型和柯萨奇B5型。

Results The concentration of Chlorogenic acid was 0.05mg/ml, 0.1 mg/ml,0.4mg/ml,0.8mg/ml respectively,it can respectively inhibit respiratory syncytia virus,Coxsackie B 3 virus,adeno associated 7 virus,adeno associated 3 virus and Coxsackie B 5 vi...

结果 氯原酸浓度分别为 0 。0 5mg/ml、0 。1mg/ml、0 。4mg/ml、0 。8mg/ml时，可分别体外抑制合胞病毒、柯萨奇B3、腺病毒 7型、腺病毒 3型和柯萨奇B5型。结论氯原酸对常见呼吸道病毒有较强的抑制作用

You can buy sweetened beverages, if that suits you.

你若喜欢的话，可以买些含有糖精的混合饮料。

As many of you are probably aware, Nick and Vanessa were recently faced with an embarrasing sex scandal after naked pictures of the two getting jiggy with it in a hot tub surfaced the net.

正如很多你可能知道，尼克和 Vanessa 最近面临一个尴尬的绯闻事件后，赤裸裸的图片两个越来越jiggy与它在浴缸中的一个热点浮现净。