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The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.
结果显示:先证者尿检呈强阳性;其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变,为半合子突变,而其母、其姐为杂合突变;正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变;不同物种氨基酸序列的同源性比对显示: c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守;酶活性测定的结果显示:先证者的IDS酶活性仅为2.3 nmol/4 h/mL,大大低于正常值,而其父的为641.9 nmol/4 h/mL,其母的血浆酶活性为95.8 nmol/ 4h/mL,其姐的为103.2 nmol/4 h/mL。
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Pharmacodynamic parameters were calculated as follows: Tmax 0.74 h, C max 0.92 μg·mL-1, t 1/2α 0.68 h, t 1/2β 20.46 h, V/F 87.39 mL, CL 5.85 mL��h -1 (0.10 mL��min -1). On the other hand, after oral administration AA-I was rapidly distributed to all the viscera or tissue, whose peak appeared in 5 minutes and the vallecula was from 24 to 48 hours.
经拟合其特征符合血管外给药二室模型,所得参数显示:口服AA-I在 0 。74h达峰,达峰浓度 0.92mg·L-1,分布半衰期( t 1/ 2α) 0.6 8h ,消除半衰期( t 1/2β) 20.46h ,表观分布容积 87.39mL ,总清除率CL 5 。85mL·h-1(0.10mL·min-1)。
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The results showed that supplying lysine coated with the polyoxylateⅣand hydrogenated vegetable tallow in the rumen could significantly improve the concentration of plasma free lysine in the jugular vein (11.18ug/ml)(P<0.05), which is about 66.61% of that with duodenal infusions of lysine(16.97ug/ml). The concentrations of free lysine were 7.31ug/ml and 8.00ug/ml with the basal diets and supplementing of Lys in the rumen respectively.
选用4头瘘管肉牛,采用4×4拉丁方设计,结果表明,瘤胃补充用丙烯酸树脂Ⅳ号和氢化植物油保护的赖氨酸26g(15g赖氨酸),可显著提高肉牛血浆游离赖氨酸浓度(11.18ug/ml),为十二指肠灌注15g赖氨酸(16.97ug/ml)的66.61%,基础日粮和瘤胃补充15g赖氨酸血浆游离赖氨酸浓度分别为7.31ug/ml,8.00ug/ml。
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The results showed that:The best zymolysis conditions for BS and TV protoplasts release and regeneration through orthogonal experiment were as follows: 1mg·mL~(-1) lysozyme treated BS protoplasts for 1.5 hours at 37℃ and 4 mg·mL~(-1) lywallzyme treated TV protoplasts for 2 hours at 30℃. In these conditions, the number of protoplasts release was respectively 8.87×10~8 units/mL and 8.62×10~7units/mL and the regeneration rate was respectively 54.1% and 29.3%.
研究结果表明:通过正交试验确定BS菌和TV菌原生质体制备过程中的最佳酶解条件分别为:BS菌在37℃酶解温度下,1mg/mL溶菌酶酶解1.5h,原生质体制备个数达到8.87×10~8个/mL,再生率为54.1%;TV菌在30℃酶解温度下,4mg/mL破壁酶酶解2h,原生质体制备个数为8.62×10~7个/mL,再生率为29.3%。
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240V and 50Hz Luer lock-type reusable needles, metal hub,18 gauge, 40mm, box of 100 Luer lock-type reusable needles, metal hub, 16 gauge, 25mm, box of 100 Luer lock-type reusable needles, metal hub, 14 gauge, 25mm, box of 100 Luer lock-type reusable needles, metal hub, 20 gauge, 30mm, box of 100 Intravenous catheters, 16 gauge, 50mm (2"0) 3 litre intravenous fluid administration bags, with attached tube Simplex IV Bell or equal, high quality latex, 10cm (4") Tubing Kelley Forceps 14 cm, curved Capacity 1 litre, max 1,000 rpm Mayo Dissecting Scissors 14 cm, curved, standard bevelled blades or equal Metal pre-set multi-dose syringe to deliver 0.1ml, with accessories and spares Batch operation for food/meat Portable, for internal determination of foreign bodies in livestock, and magnetic retractor Multi-dose Syringe, 30 ml capacity, adjustable dose of 1-30 ml Henke Vetmatic metal multi-dose syringes, capacity 10 ml with tube feed Nasogastric tubes, 13mm (1/2) outside diameter abd 2.5m (8 feet) long 'Record' type intr-dermal needles as recommended for mclintock syringe or similar Oral dosing syringe for deworming connect various sizes of supply bottles, adjustable dose of 1 to 50 ml Parasite control sprayer back-pack, minimum capacity 10 litre, hand pump for pressure delivery without taking off back.
- 240V的和50 Luer锁型可重复使用的针头,金属枢纽, 18针, 40毫米, 100盒 Luer锁型可重复使用的针头,金属枢纽, 16个指标, 25毫米, 100盒 Luer锁型可重复使用的针头,金属枢纽, 14个指标, 25毫米, 100盒 Luer锁型可重复使用的针头,金属枢纽, 20个指标, 30毫米, 100盒静脉导管, 16个指标,使用50mm ( 2 & 0 ) 3升静脉输液袋,附有管单纯四贝尔或等于高品质乳胶, 10厘米( 4 &)油管凯利钳十四厘米,弯曲容量1公升,最大转速1000 梅奥解剖剪刀14厘米,弯曲,标准斜切刀片或等于金属预先设定多剂量注射器提供0.1毫升,与配件和备件批量操作的食物/肉便携式,内部确定异物牲畜和磁缩多剂量注射器, 30毫升的能力,可调节剂量的1月30日毫升亨克Vetmatic金属多剂量注射器,容量10毫升管饲料鼻饲管, 13毫米( 1 / 2 )外径阿卜德250 ( 8英尺)长'记录'类型中断真皮针头作为建议麦克林托克注射器或类似口服注射器的驱虫连接各种尺寸的供应瓶,可调剂量为1至50毫升寄生虫控制喷雾器回包,最小容量10升,手泵的压力下交付起飞返回。
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Results: the extracts of dipsacus asperoids with different concentration(1ug/ml��� 10ug/mk、 100ug/ml) can increase the ability of osteoblastic proliferation; the extracts of dipsacus asperoids with concentration of 10ug/ml and 100ug/ml can increase the ability of osteoblastic differentiation.
结果:浓度为1ug/ml、10ug/ml和100ug/ml的续断甙溶液有促进成骨细胞增殖的作用;浓度为10ug/ml和100ug/ml的续断甙溶液有促进成骨细胞ALP分泌的作用。
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Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.
结果:酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间,给药剂量为80μg/kg/d时疗效最显著,达到69.24%,在20μg/kg/d-80μg/kg/d剂量范围内生命延长率为50-70%,给药剂量与荷瘤鼠生存时间呈现一定量效关系;酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长,给药剂量为160μg/kg/d时疗效最显著,抑制率为44.03%,并且在40-320μg/kg/d剂量范围内抑制率为25-50%,给药剂量与肿瘤抑制率呈现一定量效关系;酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用,在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显,其中浓度为100μg/ml时抑制率达19.36%;酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤:体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强,与效应细胞对照组相比有显著性差异(P<0.05)杀伤率分别达到35.58%、61.2%;体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强,与生理盐水对照组相比有显著性差异(P 。05),杀伤率分别达到21.39%、47.63%;裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强,与生理盐水对照组相比有显著性差异(P.05),最高杀伤率分别达到32.86%、73.07%;酪丝亮肤能增强单核巨噬细胞系统的吞噬功能,吞噬指数与生理盐水组比较有显著性差异(P.05);酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO,与效应细胞对照组相比有显著性差异(P.05);酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO,与生理盐水对照组相比有显著性差异(P.05);酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO,IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰,酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能,而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。
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Mean basal metabolic rates within thermal neutral zone were 3.65±0.14 ml O2/ for buntings,4.69±0.27 ml O2/ for white-eyes,3.55±0.14 ml O2/ for hwameis,and 4.24±0.17 ml O2/ for leiothrix,which are 128%,230%,60% and 120% of the expected value based on their body mass,respectively.
在热中性区内,黄眉NFDA2、红胁绣眼鸟、画眉和红嘴相思鸟的平均基础代谢率分别是3.65±0.14、4.69±0.27、3.55±0.14和4.24±0.17ml O2/,分别是体重预期值的128%、230%、60%和120%。
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Under lucifugal condition,the ED50 and ED90 of direct virucidal activity of it to HSV-1F strain are 12.90 μg/ml and 26.20 μg/ml separately,the ED50 and ED90 of infection block action of it to HSV-1F strain are 24.13 μg/ml and 32.06 μg/ml respectively.
在避光条件下,对HSV-1F株直接杀灭的ED50及ED90分别为12.90 μg/ml和26.20 μg/ml;感染阻断的ED50和ED90依次为24.13 μg/ml以及32.06 μg/ml。
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Under lucifugal condition,the ED50 and ED90 of direct virucidal activity of it to HSV-1F strain are 12.90 μg/ml and 26.20 μg/ml separately,the ED50 and ED90 of infection block action of it to HSV-1F strain are 24.13 μg/ml and 32.06 μg/ml respectively.
结果 在Vero细胞系统中,C60在光照及避光条件下,对HSV-1F株均具有直接杀灭和感染阻断作用,不具有增殖抑制作用。
- 相关中文对照歌词
- Gods Don't Chill
- Lord Have Mercy
- Avant De Partir
- Turn Around (5, 4, 3, 2, 1)
- Marche Ou Crève
- Wat Da Hook Gon Be
- Heaven For A Gangsta
- Cocoa Leaf (No Relief)
- Hear Me Coming
- 推荐网络例句
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You can buy sweetened beverages, if that suits you.
你若喜欢的话,可以买些含有糖精的混合饮料。
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As many of you are probably aware, Nick and Vanessa were recently faced with an embarrasing sex scandal after naked pictures of the two getting jiggy with it in a hot tub surfaced the net.
正如很多你可能知道,尼克和 Vanessa 最近面临一个尴尬的绯闻事件后,赤裸裸的图片两个越来越jiggy与它在浴缸中的一个热点浮现净。
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The difference. But you don抰 use diagonals when photographing do you.
所以,如果你要摄影的话,请不要用天顶镜。