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By chicken embryo, minimum concentration of medicine of directly inactivated test、 treatment test and preventive test is 7.5mg/ml��� 62.5mg/mland 31.25mg/ml;By CEF, minimum concentration of resiting NDVF_(48)E_8 and moderate virulence strain is 10mg/ml��� 50mg/ml and 25mg/ml. However,the result of complex prescriptionpreventing and curing NDVF48Ej$ is not ideal.

在鸡胚上抗病毒试验中，对三株病毒起直接灭活、治疗和预防作用的最低药物浓度分别为7.5mg/ml、62.5mg/ml和31.25mg/ml；在鸡胚成纤维细胞上抗病毒试验中，对强强毒株和中等毒力株起直接灭活、治疗和预防作用不引起细胞病变的最低药物浓度分别为10mg/ml、50mg/ml和25mg/ml。

HL60 cells with same density was divided into 5 groups at random , added with 0 μ g/ml, 5 μg/ml, 25 μg/ml, 50 μg/ml and 75 μ g/ml ChS.

同一密度的HL60细胞随机分为五组，加入0μ g/ml、5μ g/ml、25 μ g/ml、50 μ g/ml和75 μ g/ml ChS。

The recoveries of chlortetracycline (0.01μg/mL���0.10μg/mL���0.20μg/mL���0.40μg/mL���2.00μg/mL) in muscle, liver and kidney were 92％～101％;:88％～04％; 79％～86％, respectively.

金霉素在0.01μg／mL、0.10μg／mL、0.20μg／mL、0.40μg／mL、2.00μg／mL五个添加水平上的平均回收率分别为：肌肉：92％～101％；肝：88％～94％；肾：79％～86％。

The concentration of antibody is 1:600,the 1:400 dilution of antigen is 160 mU/mL,the suitable resule of enzyme is 1:3000.The range of the kit is 5～160 mU/mL,sensitivity is 0.46mU/mL,recovery of the high and low concentration are 106.74%and 104.78%,accuracy rating is 38.982mU/mL,stability is perfect, variation is less than 15%.

本试剂盒的范围为5～160 mU/mL，足以包含正常范围6～25mU/mL；灵敏度为0.46mU/mL；高浓度样品平均回收率为106.74%，低浓度样品平均回收率为104.78%；准确度为38.982mU/mL；稳定性良好；精密度实验结果显示，批内变异＜10%，批间变异＜15%。

An aliquot of plasma (0.4 mL) was added to 0.4 mL of water, and the resulting solution was applied to a previously activated (3 mL of methanol followed by 6 mL of water) 100-mg Oasys cartridge.

一个aliquot血浆（ 0.4毫升）加0.4毫升水，以及由此产生的解决方案适用于先前激活（ 3毫升的甲醇其次是6毫升水） 100毫克Oasys墨盒。

The SPE-eluent was prepared just before use and consisted of a mixture of 2.5 ml 25% ammonium hydroxide, 2.5 ml 1.0 mg/ml methanolic ascorbic acid and 45 ml methanol.

SPE洗提液在用途之前准备了并且包括了2.5 ml 25%氢氧化铵， 2.5 ml 1.0 mg/ml methanolic抗坏血酸和45 ml混合物甲醇。

Results The positive detecting rate of aerobe bottle against aerobe was 100%, Sensitivity was 10 CFU/ml. The positive detecting of anaerobe bottle against anaerobe was 100%, Sensitivity against peptococcus niger, Peptostreptococcus anerobius, bacteroides fragilis was 10 CFU/ml, 100 CFU/ml, 1000 CFU/ml, respectively.

结果 需氧瓶对需氧菌的阳性检测率为100%，检测敏感性为10 CFU/ml；厌氧瓶对厌氧菌的阳性检测率为100%，对黑色消化球菌、厌氧消化链球菌和脆弱类杆菌的检测敏感性分别为10 CFU/ml、100 CFU/ml和1 000 CFU/ml；厌氧瓶对兼性厌氧菌的阳性检测率为100%，检测敏感性为10 CFU/ml。

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

When the strain grew in the medium containing glucose(40 g/L) as carbon source with tomato juice(3.6 mL/L), gingili oil(1.6 mL/L), Na2CO3(6 g/L)and phosphate buffer, at 28 ℃ with illumination for 120 h, its cell biomass, and carotenoid yield and content could reach 65.57 mg wet cell per mL medium, 9.896 μg per mL medium and 150.9 μg per gram wet cell, respectively.

菌株在以葡萄糖为碳源，添加番茄汁（3.6 mL /L）、芝麻油（1.6 mL/L）、Na2CO3（6 g/L）和磷酸缓冲盐的液体培养基中28 ℃振荡培养5 d，每mL培养液细胞生物量湿重达到65.57 mg，类胡萝卜素产量达到9.896 μg，菌体每g湿重细胞胡萝卜素含量为150.9 μg。

SODIUM GLYCOLATE— Transfer about 500 mg of it, accurately weighed, into a 100-mL beaker, moisten thoroughly with 5 mL of glacial acetic acid, followed by 5 mL of water, and stir with a glass rod to ensure proper hydration (usually about 15 minutes). Slowly add 50 mL of acetone while stirring, then add 1 g of sodium chloride, and stir for several minutes to ensure complete precipitation of the carboxymethylcellulose.

乙醇酸钠—取本品500mg，精密称定，置于100mL烧杯中，用5mL冰乙酸完全润湿，接着加5mL水，并用玻棒搅拌使水合作用完全（搅拌时间一般为15分钟），边振摇边缓缓加入50mL丙酮，再加入1g氯化钠，振摇几分钟以确保羧甲基纤维素沉淀完全。

You can buy sweetened beverages, if that suits you.

你若喜欢的话，可以买些含有糖精的混合饮料。

As many of you are probably aware, Nick and Vanessa were recently faced with an embarrasing sex scandal after naked pictures of the two getting jiggy with it in a hot tub surfaced the net.

正如很多你可能知道，尼克和 Vanessa 最近面临一个尴尬的绯闻事件后，赤裸裸的图片两个越来越jiggy与它在浴缸中的一个热点浮现净。