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Transfer 50 ml of this solution to 200-ml Erlenmeyer flask, or, if smaller aliquot is taken, dilute to about 50 ml with water. Add 10 ml 4 N H2SO4, 1 ml KI,1 drop ammonium molybdate solution, and 50 ml water. With continuous agitation, add excess of 0.00359 N Na2S2O3 (5 10 ml).

移取50毫升上述溶液于200毫升于锥形瓶中，如果取样量较少，用水稀释到50 毫升，加入10 ml 4 N H2SO4，1 ml KI， 1滴钼酸胺溶液和50毫升水，边搅拌边加入过量的0.00359 N Na2S2O3溶液(5 10 ml)。

The irrigates were Chinese nutgall（200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL, 12.5 mg/mL and 6.25 mg/mL���, 3 mL/L hydrogen peroxide plus physiological saline, 170 g/L EDTA plus 52.5 g/L NaCl0 and distilled water.

72个离体单根牙随机分为9组，冲洗液为五倍子组（分别为200、100、50、25、12.5、6.25mg／mL）、30mL／L过氧化氢液加生理盐水组、170g／LEDTA加52.5g／LNaOCl组、双蒸水组。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Rat in Group B was set an air bladder which was empty and not perfused water. Water of 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml, 1.4 ml and 1.5 ml was perfused respectively into the air bladders which were set in the colon of rats in group C, D, E, F, G and H. Then the changes of ethology of the rats were recorded in interval of an hour.

应用疼痛行为学的观察方法，观察1h各组动物的行为学改变，并采用免疫荧光化学的方法，观察大鼠DCN中神经元被激活的标志c-fos及胶质细胞的胶质原纤维酸性蛋白(glial fibrillary acidic protein， GFAP)和小胶质细胞特异性补体OX42的表达情况，空白对照组是在疼痛高峰点(1.2 ml)处死取材。

Studied on sensitivities of C. ellipsoidea to kanamycin , Chloromycetin , Hygromycin , Streptomycin and Geneticin (G418), the results showed that C. ellipsoidea is not sensitive to Km, Str and Cm, 600μg/ml km, 600μg/ml Str and 200μg/ml Cm can not inhabited the growth of C. ellipsoidea. However, it is fairly sensitive to Hm, 100μg/ml can completely stop the growth of C. ellipsoidea on solid media. We also found that C. ellipsoidea was highly sensitive to G418, 30μg/ml and 15μg/ml can inhabit the growth of C.

通过5种基因工程中常用抗生素对小球藻生长抑制的研究发现：小球藻对卡那霉素、链霉素、氯霉素不敏感。600μg/ml的卡那霉素、600μg/ml的链霉素和200μg/ml的氯霉素仍不能抑制小球藻的生长；小球藻对潮霉素较为敏感，100μg/ml的用量可抑制固体培养中小球藻的生长；而对G418表现出高度敏感性，30μg/ml的G418即可完全抑制固体培养中小球藻的生长，15μg/ml的G418即可完全抑制液体培养中小球藻的生长。

Results The levels of TAFI: Ag and TAFIa: Ag were significantly higher (P.01) in patients with IS ([118.72±31.41] μg/mL, [168.79±55.36] ng/mL) and ICH ([127.51±37.59] μg/mL, [207.99±73.71] ng/mL) as onset than in control ([100.63±25.28] μg/mL,[126.43±31.88] ng/mL) and the incidence rate was higher. For evaluating the risk of stroke with TAFI, the odds rate for TAFIa: Ag was about 3 times in IS compared with control, and was 7 times in ICH.

结果 与对照组[TAFI： Ag(100.63±25.28)μg/mL； TAFIa： Ag(126.43±31.88) ng/mL]相比，卒中发作时，2指标在IS[(118.72±31.41)μg/mL，(168.79±55.36) ng/mL]和ICH[(127.51±37.59)μg/mL，(207.99±73.71)ng/mL]均明显升高(P.01)，并有较高发生率；TAFI评估脑卒中发病风险时，TAFIa： Ag在IS是对照组的3倍，在ICH是对照组的7倍。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

The soluble peptidoglycan is gained by short dose benzylpenicillin sodium and sephadex G-100; the SLP reagent kit revealed the prepared soluble peptidoglycan is peptidoglycan; the limulus test showed that the content of LPS in the prepared soluble peptidoglycan(10ng/ml) is lower (0.01ng/ml); the improved LOWRY reagent kit showed the content of protein in the prepared soluble peptidoglycan(1.0mg/ml) was 0.06mg/ml; the optimization condition is 0.75mg/ml of the dosage of benzylpenicillin sodium,1h of the incubation time of benzylpenicillin sodium addition and 5×108CFU/ml of the bacterial concentration.

应用青霉素诱导和Sephadex G-100凝胶柱成功分离得到金葡菌可溶性PGN；所制备的可溶性PGN经SLP试剂检测结果为阳性；动态浊度鲎实验检测制备的可溶性PGN(10ng/ml)中LPS含量小于0.01ng/ml；改良LOWRY试剂检测可溶性PGN(1.0mg/ml)中蛋白含量为0.06mg/ml；青霉素诱导和Sephadex G-100凝胶柱法提取金葡菌可溶性PGN的最佳条件是：青霉素的使用浓度为0.75mg/ml，菌液浓度为5×108CFU/ml，加入青霉素后孵育时间为1h。

According to the ability of the field isolates of Gibberella zeae to grow on the PSA with varying carbendazim concentrations, three sensitivity levels of isolates were determined in vitro. The sensitive isolates could grow at 0.5 μg/ml, but could not grow at 100 μg/ml. The high resistant isloates could grow faster than R at 50 μg/ml, and also could grow at 100 μg/ml.No low resistant isolates, that could grow fast at 1.4 μg/ml but could not grow at 50 μg/ml, were found among the field isolates.

根据在0.5、1.4、50、100 μg/ml等不同浓度的含药PSA平板上能否生长，将玉蜀黍赤霉田间菌株对多菌灵的敏感性划分为：敏感、中抗和高抗等3个水平，其中S菌株在0.5 μg/ml浓度下能生长，但在≥1.4 μg/ml浓度下生长受到完全抑制；R菌株在1.4 μg/ml浓度下能快速生长，在50 μg/ml浓度下能缓慢生长，但在≥100 μg/ml浓度下不能生长；HR在≥100 μg/ml浓度下仍能生长。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

You can buy sweetened beverages, if that suits you.

你若喜欢的话，可以买些含有糖精的混合饮料。

As many of you are probably aware, Nick and Vanessa were recently faced with an embarrasing sex scandal after naked pictures of the two getting jiggy with it in a hot tub surfaced the net.

正如很多你可能知道，尼克和 Vanessa 最近面临一个尴尬的绯闻事件后，赤裸裸的图片两个越来越jiggy与它在浴缸中的一个热点浮现净。