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Manganese superoxide dismutase is an important antioxidation enzyme in mitochondria. Is its expression inhibited in hyperglycemia? Uncoupling proteins belong to the mitochondrial anion carrier gene family and, as suggested by their name, can uncouple ATP production from mitochondrial respiration by causing proton leak.

作为最主要的线粒体抗氧化酶—线粒体超氧化物岐化酶(manganese superoxide dismutase，MnSOD)，在高血糖条件下，其表达或活性是否受到了抑制从而导致了活性氧(reactive oxygen species，ROS)产生的增加？

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

Japonicus used in this experimentation is matemal inheritance.5 Sequence analysis of mitochondrial 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber500 bp 16S rRNA and 540 bp COI fragment were obtained by PCR method from mitochondrial DNA of four species of sea cucumber, Apostichopus japonicus, Holothuria fuscogilva, Cucumaria frondosa and Mensamaria intercedens. Variable sites, nucleotide diversity, average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference, and phylogenetic trees were constructed by the NJ and ME method.

结果显示在来自这5组家系的100个后代中仅检测到母本的单倍型，刺参的线粒体遗传模式表现为典型的母系遗传。5 4种海参16S rRNA和COI基因片段序列比较及系统学研究采用PCR技术对山东荣成、长岛、俄罗斯和日本的刺参，黄乳海参，北大西洋瓜参，二色桌片参的16S rRNA和COI基因片段进行了扩增和测序，分别得到了长度约为500 bp和540 bp的片段。

Yangtsekiense populations. 1. The mitochondrial genome S. yangtsekiense and phylogentic studies of Decapoda We determined the complete 11, 405 bp mitochondrial DNA sequence of S.

长江华溪蟹线粒体基因组及十足目系统发生研究测定了长江华溪蟹线粒体基因组11， 405 bp的核苷酸序列，包括11条蛋白编码基因和17条tRNA基因。

mitochondrial is the only organelle containing extranuclear genetic material in mammal cell, which proper function required extensive nuclear-mitochondrial interactions.

线粒体是哺乳动物细胞中唯一存在有核外遗传物质的细胞器，其功能的正常表达需要核与线粒体的相互作用。

The combined analysis of the four cpDNA sequences showed similar topology to that in matK trees.2 Mitochondrial matR gene sequence analysisThe mitochondrial gene matR of 28 species representing 28 genera of Fagales, and 2 outgroups was sequenced.

3基于核核糖体185 rDNA序列的分析在185 rRNA基因最简约树的严格一致树上，壳斗目被分为两支，一支由壳斗科和南青冈科组成，另一支由核心高等金缕梅类组成，BS支持率均不高。

Using the intact G1-nuclei prepared from an isogenic yeast strain lacking mitochondrial DNA , we demonstrated that the DNA synthesis observed within the first 10-20 min was largely due to mitochondrial DNA synthesis, whereas nuclear DNA synthesis did not begin until after a 10-20 min lag period.

利用从缺少线粒体DNA的菌株中制备的完整G1期细胞核，证明了最初10-20min的滞后期内的DNA合成主要是由线粒体DNA合成造成的，而核DNA合成是在经过10-20 min后才开始。

Mitochondrial Eve is the name given by researchers to the woman who is the matrilineal most recent common ancestor for all living humans, from whom all mitochondrial DNA in living humans is descended.

线粒体夏娃是研究人员为一位是所有人类最亲近的共同母系祖先的妇女命名的，人类从她那里遗传了所有的线粒体DNA。

In order to explore the role of cytochrome c oxidase during the development of Microtus brandti, the activity of mitochondrial CcO and content of mitochondrial protein in voles＇ liver was measured by using ultraviolet spectrophotometry, these voles were chosen according to days.

为探讨线粒体能量代谢中细胞色素c氧化酶（cytochrome c oxidase，CcO）在布氏田鼠生长发育过程中的作用，应用紫外分光光度法测定了不同日龄的幼鼠肝脏内线粒体蛋白含量及CcO活性。

A shot-gun DNA sequence strategy was employed,in which the mitochondrial genome library of Muntiacus reevesi has been constructed to obtain the complete mitochondrial genome sequence.

通过建立麂属动物小麂线粒体DNA文库、鸟枪法测序，获得了小麂线粒体基因组全序列并对其基因组成、蛋白质的编码序列、tRNA基因等结构作了详细分析，这也是国内有关哺乳动物线粒体基因组全序列的首次报道。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。