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For quantitatie real-time PCR of DR4 and DR5 transcripts, triplicated samples containing 9 l of cDNA with 10 l of Taqman Uniersal PCR Master Mix (Applied Biosystems, Foster City, CA) and 1 l of 20xAssays-on-demand Gene Expression Product (DR4; Hs 00269492_m1, DR5; Hs 00366272_m1, Applied Biosystems) were pre-incubated at 50oC for 2 minutes and subsequently at 95oC for 10 minutes.

对于进行定量实时PCR的DR4 和 DR5转录产物，使用10L 美国应用生物系统公司生产的 Taqman Uniersal PCR Master Mix试剂，1L的20xAssays-on-demand Gene Expression Product (美国应用生物系统公司生产的DR4； Hs 00269492_m1， DR5； Hs 00366272_m1)，先将其在50度下孵化2分钟，然后在95度下孵化10分钟，用这两种试剂将含有9L cDNA的样品扩增两倍。

Only 15 minutes to 20 minutes is required to neutralize weak acidic water into alkaline reduction water.

只需15-20分钟，就能把中性的自来水和弱酸性的逆渗透水转化为碱性还原水。

September 24, 2007, 7:08 amJason Kidd played fewer minutes than anyone anticipated during last month's Americas Cup blowouts in Las Vegas. With no team near the USA's combine, and with two top backups, Kidd played only 15 minutes per game.

上个月在Las Vegas举行的美洲杯比赛中，由于美国队实力超群加上组织后卫位置上阵容强大，Jason Kidd每场比赛只需要在场上待15分钟。

The average time of extracorporeal circulation was 172 minutes and the average time of clamping ascending aorta was 116 minutes.

目的 探索重症瓣膜替换术体外循环管理和心肌保护的方法。

F Fly Fish, banana Boat for 15 minutes, Jet ski for 15 minutes, Snorkling only

飞鱼，香蕉船15分钟，摩托艇15分钟，浮浅

Just remember that there is a world of ice and snow called明教, running very quickly, into a good little primer to say, the various break, after the horse finished the bridge to kill, but also only 19-20 minutes, four minutes after waiting for three In addition to two on five out of the brush to bear.

只记得有个明教叫冰天雪地，跑很快，进去就说好少引点，各个击破，桥上杀完马后，还只有19-20分钟，等了4分钟以后，三下五除二就出来了去刷熊。

General experience is that at room temperature about 20 degrees when a well-ventilated room, Internet browsing, Centrino notebook CPU fan, it should be in every 20-30 minutes to start time, about 5 minutes to stop the work of energy conservation work.

一般的经验就是在室温二十度左右的时候，室内通风良好，上网浏览网页，迅驰CPU的笔记本风扇，应该在隔二十到三十分钟启动一次，工作五分钟左右停止工作节能。

Bake until cakes are golden and tester inserted into center comes out clean, about 25 minutes. Cool in pans on racks 15 minutes. Turn out onto racks, peel off parchment, and cool completely.

将作好的蛋糕放如烤炉烤至金黄，中央微微隆起时（约25分钟）拿出，放于烤盘架子上晾凉15分钟，然后置于托盘上，撕去防油纸，让其彻底晾凉。

Forty minutes and eighty minutes are needed for the filter chamber filled with sand to recover the ability of Fe and Mn removal after backwash.

反冲洗之后，河砂滤柱对铁和锰的去除能力需要40 min和80min才能完全恢复；锰砂滤柱对铁、锰的去除能力需要20 min和40 min才能完全恢复。

The method adopts powders (10.0 parts of mass) of crop straws and phenols or polyalcohol compound (10 to 100 parts of mass) which is firstly pre-heated by 10 to 30 minutes under a temperature of 30 to 200 DEG C with the effect of acid catalyst (0.5 to 10 parts of mass) for a reaction of 30 to 240 minutes; after the reaction, the compound is washed, neutralized, filtered and dried to obtain a solid appearing as a color from citron yellow, yellow, bight brown to seal brown; wherein, the solid includes 64.0 to 70,0 percent of fibrin and 4.1 to 7.6 percent of lignin.

秸秆&液化&预处理制备生产燃料乙醇原料的方法，是用农作物秸秆的粉末(10.0质量份)和酚类或多元醇类化合物(10～100质量份)，在酸性催化剂(0.5～10质量份)存在下，在30～200℃温度下预热10～30min后，反应30～240min，反应完毕进行洗涤、中和、过滤和干燥而得呈淡黄色-黄色-浅褐色至深褐色固体，其中，纤维素含量64.0～70.0％，木质素含量4.1～7.6％。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。