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In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只,每组再分为高代谢脓毒症组和对照组两部分,建模方法同第一部分;2)三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸;3)灌注前抽取动脉血作为背景值,灌注达150,160,170和180min分别抽取动脉血2ml用于质谱分析;4)实验开始后前30min中每5min收集呼出气一次,随后每30min收集呼出气一次,用于气相质谱分析;5)血样经过低温离心取上清液,过阳离子及阴离子交换树脂,再经衍生化处理;6)处理过的血样进入气相色谱-质谱仪,测量其13C标记的亮氨酸、葡萄糖和乳酸丰度;7)实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度(E13CO2);8)利用文献提供的公式算出CO2总生成率(V13CO2)以及机体各物质的通量、氧化速率以及物质代谢百分比等;9)数据分析处理采用t检验。

Kopin et al, estimate that exogenously administered melatonin disappears from whole mice with a half-life of about 2 min through the first 10 min following injection but that after 40 min the half-life has grown to about 35 min.

Kopin等人,估计外部褪黑素从整个小鼠消失的半衰期在注射后的最初10分钟里,大约为2分钟,但注射后40分钟后半衰期就增长到约35分钟。

On the basis of these treatments, the patients in the treatment group also had aerobic kinesitherapy through limb and trunk exercises with assistance to improve oxygen consumption of the body and increase the heart rate. In each session of exercise, the patients were supposed to attain the target heart rate of 70% to 85% of the personal highest safe heart rate.At the beginning, each session of exercise lasted for 10-15 minutes and the heart rate or pulse was measured every 4-6 minutes, with the patients'heart rate allowed to increase by no more than 20/minute and the patients not feeling tired. The subsequent exercise time gradually increased but not over 30-40 minutes, twice a day for no less than 5 days a week.

干预:两组患者均给予改善循环和营养神经药物等治疗,在此基础上,治疗组采用有氧运动疗法:以靶心率为指标,即以通过运动获得的个人最高安全心率70%~85%作为每次运动的靶心率,开始时每次运动10~15 min,每运动4~6 min测一次心率或脉搏,运动以患者心率增加不超过20次/min、患者感觉不疲乏为度,以后逐渐增加运动时间,直至每次活动持续时间30-40 min,2次/d,每周治疗不少于5 d。

Both dosages of DIZ (2 mg/kg) and MID (2 mg/kg) with an increased KET dosage (from 60 to 90 mg/kg) shortened the time of diminished corneal reflex (from 5.3 ± 0.8 to 2.2 ± 0.3 min) and palpebral reflex (from 3.0 ± 0.4 to 1.3 ± 0.2 min) and significantly prolonged the duration of anesthesia (from 97.2 ± 18.0 to 187.8 ± 28.8 min vs. from 103.3 ± 9.4 to 181.8 ± 19.5 min), but did not reduce the recovery time.

於定量DIZ(2 mg/kg)和MID(2 mg/kg),KET由60 mg/kg增加至90 mg/kg,可分别缩短导入期角膜反射减弱(由5.3 ± 0.8分钟减至2.2 ± 0.3分钟)与眼睑反射减弱(由3.0 ± 0.4分钟减至1.3 ± 0.2分钟)时间,且能显著延长麻醉时间(分别由97.2 ± 18.0分钟增至187.8 ± 28.8分钟和由103.3 ± 9.4分钟增至181.8 ± 19.5分钟),但并未延长回复期。

Physical examination:T38 C, R32/min, P120/min, dyspyoria, Fontanel 1.5×1.5cm2, sunken, orbit sunken, decreasedSkin turgor, dry lip, dry periglottis, pharynx, heart rate 120/min, no arrhythmia, mild dull heart sounds, lungs, mild abdomen swelling, soft abdomen, not palpated liver 1.5cm below ribs, bowel sounds 10~12/min, no high notes,two lower limbs patellar reflex negative

体查:T38 C,R32次/分,P120次/分,烦躁不安,体查不合作,前稳囟1.5×1.5cm2,凹陷,眼眶凹陷,皮肤弹性差,唇干燥,舌粘膜干燥,咽,心率120次/分,率齐,心音稍钝,双肺,腹稍隆起,腹软,肝肋下1.5cm未扪及,肠鸣音10~12次/分,未闻及高调音,双下肢膝腱反射阴性。

Methods Sixtyeight patients with serious head injury receiving oxygen therapy were randomly divided into 2 groups, rhinophore group and venoclysis group. The heart rate, blood pressure and blood oxygen pressures were observed before treatment and 30 min, 45 min, 60 min, 90 min after the treatment.

随机将68例重型颅脑损伤病人分成对照组(35例)和观察组(33例),在常规治疗的同时,对照组采用传统的鼻导管给氧;观察组采用舒氧康静脉高压给氧。

The blood gas and electrolytes from IVC and SVC were measured before operation (T0), 5 min before cross-clamping of the portal vein (T1), 5 min before unclamped of portal vein (T2), 15 min (T3) and 30 min (T4) in neohepatic phase.

于开腹前(T0)、无肝期前5min(T1)、新肝开放前5min(T2)、新肝期15min(T3)、新肝期30min(T4),分别从右颈内静脉、右股静脉导管抽取上、下腔静脉血,进行血气、电解质分析。

Results(1)At concentration of 0.002 5%,the KT50 is 4 min :0.002 5% is 3.77(3.40-4.15)min ;0.005% is 3.17( 2.88- 3.48)min;0.01% is 3.45(3.25-3.65)min ;(2)the LC50 is 0.000 530 9%(0.000 490 0%-0.000 575 2%);(3)the 100% mortality(24 hour) with the Dagongda concentration is at the rate of 0.002 5%;(4)when used as residual smearing,Dagongda can be keep its efficacy more than 270 days under room temperature;(5)The field tests,the killing rates of 0.03% Dagongda to Xenopsylla cheopis were 80.80% for 2 days and 93.87% for 29 days respectively.

2当药物浓度在0.0025%、0.005%、0.01%时,印鼠客蚤24h死亡率均为100%。(3)持效:药膜在室温和普通湿度下存放9个月后,药物浓度在0.0025%以上时,印鼠客蚤24h死亡率均为100%。(4)现场2d灭效:使用0.03%浓度对地面游离印鼠客蚤的校正灭蚤率为80.80%。(5)现场残效(29d):使用0.03%浓度对地面游离印鼠客蚤的校正灭蚤率为93.87%。

The blood pressure,heart rate and BIS value at time points of basic value, of anesthetic induction on intubating, skin incision, 1 min,3 min,5 min and 10 min after pneumoperitoneum and cholecyst dissection were recorded, the maintaining dose of propofol,esmolol and remifentanil was recorded; the peripheral vascular blood were collected at the time points of basic value, 3 min after pneumoperitoneum and cholecyst dissection to test the serum cortisol level.

采集并记录各组术前基础值、麻醉诱导气管插管、切皮、气腹1、3、5、10min、胆囊分离时共8个时间点的血压、心率及BIS值的变化;记录各组术中异丙酚、瑞芬太尼和艾司洛尔的维持用量;在术前、气腹3min、胆囊分离时抽取外周静脉血检测血清皮质醇的浓度。

Results: The content of purines in fish decreased sharply during 10 min, decreased slowly during 10 min to 40 min, was stable during 40 min to 60 min.

结果:水煮过程中鱼肉嘌呤含量在10min内迅速降低,10~40min缓慢减低,40~60min保持稳定。

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