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To shed light on cardiac effects of the potent vasoconstrictive peptide urotensin Ⅱ, Langendorff-perfused isolated rat hearts were consecutively perfused with 0.1, 1 and 10 nmol/L UⅡ, for 5 min at each dose, followed by 5-min washout.

在正常Langendorff灌流与缺血-再灌注(停灌20 min-复灌20 min)离体大鼠心脏模型，观察尾加压素Ⅱ(urotensin Ⅱ， UⅡ)对冠脉流量、心功能和心肌代谢的影响以及心肌UⅡ受体的功能，以探讨UⅡ的心脏效应。

WANG Wei-Ting, GUO Lian-Jun, QU Ling, WANG Fang, LU Qing, QIAN Jia-Qing(Department of Pharmacology, Tongji Medical College, Huazhong Universityof Science and Technology, Wuhan 430030, China)By using decapitating, intravenous injection of saturated MgCl2 and legation of bilateral carotid arteries with vagi, theeffects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethyl- amino) propane hydrochloride on survival time in mice were studied. With the model of cerebral ischemia for 20 min and reperfusion for 10 min, effects of DDPH on the superoxide dismutases activity and malondialdehyde content in brain tissue and pathological changes were studied.

采用断颅，iv饱和MgCl2溶液及结扎双侧颈总动脉和迷走神经等法造成小鼠脑缺血模型，观察1-(2，6-二甲基苯氧基)-2-（3，4-二甲氧基苯乙氨基）丙烷盐酸盐对小鼠脑缺血后存活时间的影响；采用小鼠脑缺血（20 min）再灌注(10 min)模型，观察DDPH对脑组织超氧化物歧化酶活性，丙二醛含量及组织病理损伤的影响。

Result The processing conditions were as follow: Taking the mixture of Vc and lemon acid as antioxidant, the ratio of agarics to water was 1:60, extracting temperature was 60-80 ℃, extracting time was 60 min, the ratio of beltlines eddoes to water was 1:10, microwave was 5 min.

结果］结果表明，最佳工艺条件为：以Vc与柠檬酸混合液作护色剂；木耳与水的物料比为16：0，在6080℃浸泡60 min；香菇与水的物料比为11：0，高温微波5 min。

BQJ-34 was cultured on the biotin fermentation media for 48 hour ,a 10ml fluid medium was boiled for an hour and was centrifuged (10,000r/min , 30 min), the supernatant is filleted with the 0.22μm filters and the biotin content of filter liquid is detected by RP-HPLC based on the biological samples regression analysis, recovery, accuracy testing.

利用生物素培养基对菌株BQJ-34进行振荡培养，取48h的细菌培养液10ml置100℃条件下水浴1 h，随后在超速离心机上以10 000 r/min，30 min离心，取上清液用0.22μm滤膜过滤，用高效液相检测滤液中的生物素含量，并通过对生物素样品的回归分析和回收率、精密度试验，最终确定利用反相高效液相色谱法可用于培养液中生物素测定，其最佳检测条件是：用安捷伦Zorbax SB-C18(4.6~※250cm，5μm)色谱柱，以K_2HPO_4(0.04mol，pH2.5)和CH_3CN(二者体积比为91.5：8.5)为流动相，紫外检测波长为215nm。

The diastolic pressure, systolic pressure, mean arterial pressure, heart rate, temperature respiratory rate, end-tidal carbon dioxide partial pressure and oxygen saturation of goats were monitored with intensive care monitor and respiratory monitor during the laparoscopic splennectomy in goats and were recorded at the time of before anesthesia, after anesthesia and before insufflating, intraoperative 10, 30, 5 min after the deflation, respectively.

通过在进行腹腔镜脾脏切除术的过程中，用呼吸监护仪和重症监护仪分别于麻醉前、麻醉后－气腹前、术中10 min、术中30 min和放气后5 min监测收缩压、舒张压、平均动脉压、心率、体温、呼吸频率、呼吸末CO2分压、血氧饱和度，评价山羊腹腔镜脾脏切除术对山羊呼吸和循环系统的影响。

Decoction was percolated with 9-fold volume of 70% ethanol at the velocity of 3 mL/min and the optimal isolation technology was that the pH of curcumin sample solution was regulated to 7 by 0.01 mol/L HCl and 0.01 mol/L NaOH solution and 80% ethanol was used for elution of the velocity of 4 mL/min.

结果：提取姜黄素用9倍体积的70%乙醇，以3 mL/min的速度渗漉为最佳提取工艺；利用D101大孔树脂纯化姜黄素工序中调节上样料液pH为7，用80%乙醇以4 mL/min的速率洗脱为最佳分离工艺。

The first step: Basic PV-ERP(PCL400 ms, 300 ms) was measured firstly. Stimulated distal pulmonary vein ten minutes by maximum frequency that 1:1 pacing pulmonary vein. PV-ERP was remeasured immediately, 5 min and 10 min, respectively.

用6条成年杂种犬采用自身前后对照的方法，第一部分实验：首先分别测量起搏周长分别为300 ms、400 ms时PV-ERP；接着以1：1起搏肺静脉的最快频率刺激肺静脉10 min，分别在刺激中止后的即刻、5min、10 min时重复测量PV-ERP。

Duplicate matches after the proportion, under 15~20 MPa pressure isotropic 5 min, and under 95~100℃ sterilizes for 5 min, a kind of health soft drink was finally obtained good for both young and old.

按比例复配后，在15~20 MPa压力下均质5 min，95~100℃下杀菌5 min，最终得到一种老少皆宜的健康型软饮料。

By the study of the influence of urea concentration,reaction time and temperature on the dyeing results and washing fastness,the improved conditions were found as(1) for weak acid red: 90 ℃ dyeing for 60 min with urea 225 g/L;(2) for strong acid yellow: dyeing 50 min at 80 ℃ with urea 250 g/L and NaHSO3 8 g/L.

以尿素为基础对羊毛低温染色工艺进行了初步探讨，研究了尿素浓度、反应温度和反应时间对羊毛染色效果及耐洗牢度的影响，找出了较佳的低温染色条件：①弱酸性红，尿素225 g/L，90℃，60 min；②强酸性黄，NaHSO3 8 g/L，尿素250 g/L，80℃，50 min。

Seed was milled into four components such as bran, shorts, break flour and middling flour after air-heating at 140, 155 and 170℃ for 30 min and far-infrared heating at 200, 220 and 240℃ for 15 min respectively.

燕麦籽粒采用热风干燥140、155和170℃处理30 min；远红外烘烤200、220和240℃处理15 min 6种方式处理；用法国肖邦公司CD-2实验磨粉机制粉，测定皮粉、心粉和面粉的黏度参数。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失