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Fluorescence microscopy and flow cytometry were used to observe the activation of this regulatable vector after transfection in MFC cells.

在转染MFC细胞后，运用荧光显微镜和流式细胞技术检测该载体的调控表达。

TUNEL method was used to detected the apoptosis in the artery. Results:①The MSC cocultured with VSMC expressed smooth muscle a-actin, calponin and CD31, no cells positive for calponin and CD31 were detected in the control group; and a lot of filaments were observed in the co-cultured MSC by electron microscopy.②We gain dual-stable expression of AT2R gene medicated by doxycycline regulatable system of mesenchymal stem cells.

单独培养及VSMC条件培养液培养的MSC仅SM-α-actin表达阳性；(2)Dox-on系统由四个质粒组成：调节质粒pUHD17-1 hyg，荧光报告质粒pUHC 13-3，筛选质粒pSV2neo以及含目的基因AT2R的应答质粒pUHD10-3/AT2R，以上质粒经鉴定均与其背景资料相符合，可用于实现目的基因片段在靶细胞中可调控表达研究；(3)本实验通过连续两个回合的转染及抗生素压力筛选，经Dox诱导表达后获取出低背景、高诱导表达AT2R基因的克隆。

The formation of secondary phase in the non-equilibrium peritectic reaction was observed by precise transmission electron microscopy analysis. The microstructural evolution and phase selection regularities under different solidification velocities were constructed from the laser resolidification experiments. A new phenomenon involving two-phase coupled growth was firstly detected in the rapidly solidified Ti-Al peritectic alloy. The Ti-45Al-2Cr-2Nb alloy with fully lamellar structure ( a 2+ g ) was taken as an object to investigate the possible preparation of bulky metastable g phase materials based on both adjusting the competing nucleation relation and changing the growth kinetics condition.

首先通过过冷合金微观组织分析界定了各包晶合金熔体中各竞争相以初生相出现的过冷度区间；结合高温区的相图计算，从各相形核孕育时间角度揭示了过冷熔体中亚稳相与稳定相的竞争形核关系；通过精细微观组织分析，阐明了远离平衡时包晶反应中次生相的形成机理；在激光重凝快速凝固实验中建立了不同凝固速度下合金的组织与相形成规律，并对包晶合金中两相共生生长行为进行了深入分析；最后选取初生相为 a 的全层片 Ti-45Al-2Cr-2Nb 合金，从调整各相竞争形核关系和改变晶体生长动力学条件两个方面探索了块状亚稳 g 相材料的制备和控制。

In electron microscopy endothelial cell of capillary and mitochondrial was edematous obviously, the basement membranes were thickened. RGCs were vacuolated, chromatin of nucleus aggregated marginally decreased and densification. Some of RGCs were necrosis, nucleus were dissolved, mitochondria was edematous, the rough endoplasmic reticula decreased obviously.

电镜下视网膜毛细血管内皮细胞明显肿胀，胞体变圆，突向管腔，线粒体肿胀，空泡化，基底膜增厚，视网膜神经节细胞胞体变形，核明显固缩，核膜消失，胞浆空泡化，线粒体肿胀，细胞表面突起减少，多聚核糖体及粗面内质网显著减少。

The effect of high speed stirring on the properties of starch/PVA blend solution was studied by means of spectrophotometry,microscopy,molecule weight analysis and rheometry etc.

高速搅拌是实现淀粉与PVA溶液共混的良好方法，可明显提高淀粉PVA共混薄膜的力学性能、透明性、耐水性及生物降解性﹝1，2﹞。

Inhibition of PKCdelta by rottlerin or knockdown of TG2 protein by a TG2-specific siRNA resulted in a marked increase in autophagy shown by presence of autophagic vacuoles in the cytoplasm, formation of the acidic vesicular organelles, membrane association of microtubule-associated protein 1 light chain 3 (LC3) with autophagosomes, and a marked induction of LC3-II protein, important hallmarks of autophagy, and by electron microscopy.

抑制PKCdelta的rottlerin或击倒的TG2蛋白质的TG2特定的siRNA导致显着增加，表明自噬存在自泡在细胞质，形成酸性囊泡细胞，膜协会微管相关蛋白1轻链3（ LC3 ）与autophagosomes ，并显着诱导LC3 -Ⅱ蛋白的重要标志吞噬，并通过电子显微镜。

The lung tissue for electron microscopy was cut into section 1mm thick sagittally.

将实验动物按年龄分为14日胚、16日胚、18日胚、20日胚、生后1日、1周、1个月、3个月、6个月、18个月共10组，每组6只。

The sclerocornea tissues from the right eyes were isolated, mounted and observedunder confocal and fluorescence microscopy.

GFP基因的表达通过日常喂给水中添加Doxycycline直接激活，Doxycycline持续添加21天后停止。

The microstructures and second phases were examined by transmission electron microscopy.It is found that the specimen treated by 500 ℃/100 h has the best corrosion resistance among all the specimens.TEM analysis of the specimens tempered at 500 ℃ with different time shows that the second phase changes from the seriate strips to the banding...

用透射电镜研究了这些样品的显微组织和第二相，观测到随着保温时间延长，500℃下保温样品中的第二相由连续片层逐渐转变成带状分布的颗粒，保温时间达到100 h时，基体内析出βNb.560℃下保温样品与500℃下保温样品有相似的组织转变过程，只是时间大幅缩短，保温仅10 h时，基体已完全再结晶为等轴晶。

The effects of concentration, time on the reaction was measured by UV-visible absorption spectra , the morphology of the silver nanoparticles was characterized by transmission electron microscopy; The structure of Sesbania Gum and silver colloid was measured by Fourier transform infrared spectroscopy .

通过紫外-可见吸收光谱研究了反应物浓度、反应时间对反应的影响；通过透射电子显微镜对银纳米粒子形貌进行表征；利用傅立叶变换红外光谱仪对田(来源：A116161BC论文网www.abclunwen.com)菁胶和反应所得到的银溶胶的结构进行表征；对溶胶的拉曼光谱进行检测，结果显示溶胶具有很好的Raman信号，室温下得到的溶胶表现出银纳米粒子对田菁胶的Raman信号增强作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。