查询词典 microscopy

Beijing 100034,China(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)Abstract : Objective In order to study the effects of ATP on U937 cells. Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used.

应用ATP(0.23g/L)作用于人白血病细胞U937，(1)用白细胞计数器每日计数细胞和计算抑制率；(2)用流式细胞分析仪文献检测 ATP对U937细胞周期改变和凋亡的影响；(3)苏木精-伊红染色和电子显微镜文献检测凋亡小体的形成过程；(4)用苯胺黑染色文献检测凋亡小体的生命本性。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Different silicidation processes are employed to form NiSi, and the NiSi/Si interface corresponding to each process is studied by cross-section transmission electron microscopy.

采用不同硅化工艺制备了NiSi薄膜并用剖面透射电镜对样品的NiSi/Si界面进行了研究。

Methods::a case nodular fascitis of the leg was investigated by light microscopy and immunohistochemistry.

对1例发生在小腿的结节性筋膜炎进行光镜观察和免疫组化标记。

In this work the phycobilisomes from Nostoc flagelliforme were first carefully studied by electron microscopy and single particle analysis .

本文通过负染电镜技术和单颗粒分析的方法首次对发菜藻胆体进行了深入研究。

By us ing cryo-immunoelectron microscopy,DNA in vegetative cells of Nostoc flagelli forme was detected for the first time and nucleus area at the center of cells could be positioned.

此外，应用高压冷冻和冷冻置换的免疫标记电镜技术，首次对发菜营养细胞中的DNA进行定位，明确了核区的位置及范围。

Nanoporous gold with different pore sizes was prepared by controlling the etching time of Au/Ag alloy with concentrated nitric acid and annealing. Scanning electron microscopy and a nitrogen adsorption technique were used to characterize the NPG.

以Au/Ag合金为原料，通过控制浓硝酸的腐蚀时间再辅以退火处理得到了不同孔径的纳米多孔金，利用扫描电镜和N2气体吸附仪对孔性质进行了表征。

Results:Typical apoptotic cellular changes were found after As2O3 treatment,transmission electron microscopy showed nucleole disappeared,chromatin condensated under nucleonic membrane.

结果：经As2O3作用后VSMCs出现典型的凋亡变化，透射电镜下可见细胞核仁消失，染色质浓缩聚集于核膜下。

Domain structures in Mn-Si-Al octagonal quasicrystals have been studied by high resolution electron microscopy and image processing.

用高分辨电子显微术和图象处理研究了Mn-Si-Al八次准晶中的畴结构。

Mineral compositions and palaeoclimatic indicator of palygorskite of the Late Oligocene sediments in Xunhua basin, Qinghai,were investigated using X-ray diffraction and scanning electron microscopy methods.

采用X射线衍射和扫描电子分析方法，对青海循化盆地晚渐新世沉积物的矿物组成、坡缕石的矿物学特征以及古气候指示作用进行了深入的研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。