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The apoptotic effects of LPS are detected by various skills such as fluorescence staining, TUNEL reaction, DNA agarose gel electrophoresis, electron microscopy and fluorescence activated cell sorting. Our results demonstrated that fish lymphocytes would be apoptotic under different LPS concentrations(above 10μg/ml) and different time(above 6h) inducing, which is characterized by condensation and margination of chromatin, the formation of apoptotic bodies.

结果显示，在10μg/ml以上浓度的内毒素作用18后或以100μg/ml浓度的LPS作用6h以上，淋巴细胞即可出现明显的凋亡形态学变化，表现为染色质凝集和周边化，核断裂，形成凋亡小体，DNA发生特异降解产生典型的"梯状"电泳图谱，呈现TUNEL阳性标记等，并通过流式细胞术证实这种凋亡具有时问和剂量的依赖性。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

In light and electronic microscopy, they appeared cell shrinkage, cell density increasing and eosinophilic, aggregation of cytoplasmic organelles, nucleolus fragmentation, and chromatin margination, condensation, and fragmentation. Those changes could be detected by TUNEL method.

在光镜和电镜下癌细胞凋亡表现为细胞体积缩小，胞浆浓缩嗜酸性，胞浆出泡、细胞器缩小或减少，核仁松解，染色质聚集、靠边、浓聚、断裂，在TUNEL法检测凋亡癌细胞时也可观察到相应的形态特点。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示：VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性；经4mmol/LVPA处理MUTZ-1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞装内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

Methods The activity of PTK, PKCα,βI ,βII ,γ,ε,ξ and the active station of NF-κB in large bowel carcinoma tissues was detected by using membraneous conjugated method, laser scanning confocal microscopy and immuriohistochemistry respectively in the animal model of nude mouse transplantation tumors of large bowel carcinoma.

以大肠癌裸鼠移植瘤为动物模型，用膜结合法、激光共聚焦显微镜和免疫组织化学分别测定了大肠癌组织蛋白酪氨酸激酶、蛋白激酶Cα、βI、βⅡ、γ、ε和ξ的活性以及核因子-κB的活化状况。

After using the methenamine silver-DNA staining,15 cases of eariy molluscum contagiosumtissues were examined by electron microscopy.

用DNA银染技术显示了传染性软疣病毒形态发生发育及其过程中DNA的变化。

Composition of corrosion residuals was analyzed by means of scanning electron microscopy and electron probe microanalysis,and causes for spot corrosion were discussed and preventive measures were presented.

通过扫描电镜和电子探针显微分析等手段分析了腐蚀斑点的成分，探讨了斑点腐蚀的原因并提出了预防措施。

The worn surfaces were analyzed by means of scanning electron microscopy, X ray diffraction and electron probe microanalysis.

选用 MM- 2 0 0型摩擦磨损试验机测定了不同载荷条件下 WSi2 /Mo Si2 复合材料与 45 #钢配副的干摩擦磨损性能，采用扫描电子显微镜和 X射线衍射仪分析讨论了其磨损机理。

The worn surface was analyzed with X-ray photoelectron spectroscopy, scanning electron microscopy and electron probe microanalysis.

通过与廿二酸和硫化异丁烯的对比研究发现，在廿二酸中引入硫后，氧与硫产生了明显的协同减摩抗磨及承载作用。

The deposited films were characterized by X-ray diffraction, atomic force microscopy and field emission scanning electron microcopy to investigate the influence of the buffer layer on the crystalline quality and surface morphology.

采用X射线衍射仪、原子力显微镜和场发射扫描电子显微镜研究了缓冲层对薄膜结晶特性和表面形貌的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。